**2.1.2 Strategies that make LAMP simple and cost-effective**

Conventional LAMP reagents are supplied in liquid form, and they have to be stored below -20 °C, similar to most PCR reagents. However, because of the lack of a freezer and cold chain transportation system in most of the peripheral laboratories in developing countries, it is essential to formulate LAMP reagents, which can be stably preserved at ambient temperatures (Jorgensen et al., 2006; Aziah et al., 2007). The newly formulated LAMP reagents are dried down into the lid of the reaction tubes, thus obtaining preservation stability at ambient temperatures for more than 12 months. The dried LAMP reagents can be reconstituted quite easily by shaking the tubes after the addition of the purified DNA solution. Because the LAMP reagent for each reaction is deposited on the individual tubes in advance, there is no longer a need to prepare and dispense master-mix solutions to the reaction tubes. Thus, liquid handling using micropipette, one of the most skillful steps, becomes unnecessary in the course of the assay. Moreover, this can contribute to reduced risk of carryover contamination during the assay.
