**4.5 Magnetic** *Parkia pendula* **seed gum as a matrix for concanavalin A lectin immobilization and its application in affinity purification**

The main objective of this work was to obtain a magnetic matrix with *Parkia pendula* seed gum to covalently immobilize concanavalin A [20]. The application of this magnetic composite was to obtain glycoconjugates through affinity purification. The obtained gum in the process was magnetized and activated with NaIO4. Concanavalin A immobilized on the magnetic composite was used for the recognition of bovine serum fetuin glycoprotein. A glucose solution (300 mM) was used to carry out the election of the fetuin, and confirmation was made via SDS-PAGE.

**41**

*Magnetic Bio-Derivatives: Preparation and Their Uses in Biotechnology*

formed easily and quickly with the help of a magnetic field.

also to scientists working with applied surface science.

A lectin immobilization efficiency of 63% was achieved and 14% fetuin purification. The magnetic composite is promising, given the results obtained, to be used with magnetic polysaccharide matrix to immobilize other lectins. Because it is a magnetic system, it can be used for affinity purification, and its recovery is per-

This chapter has gathered primary and interesting information about immobilization of biomolecules, how to choose the best immobilization methodology, the support for the immobilization, and, among the magnetic materials, why iron oxide particles are highly used as a matrix to immobilization. Several physicochemical techniques were mentioned and described highlighting their features and the main information provided. Lastly, we presented some magnetic bio-derivatives with potential biotechnological applications which could be applied in other areas. So the authors hope that this material has been useful not only for the enzymologists but

This work was financially supported by the Brazilian Agencies: FACEPE (Fundação de Amparo a Ciência e a Tecnologia do Estado de Pernambuco; grant number BFP-0132-1.05/17), CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico; grant number 301830/2017-7), and CAPES (Coordenação

The authors confirm that there are no conflicts of interest in this work.

de Pessoal de Nível Superior; grant number 23038.007659/2011-26).

*DOI: http://dx.doi.org/10.5772/intechopen.85748*

**5. Conclusions**

**Acknowledgements**

**Conflict of interest**

A lectin immobilization efficiency of 63% was achieved and 14% fetuin purification. The magnetic composite is promising, given the results obtained, to be used with magnetic polysaccharide matrix to immobilize other lectins. Because it is a magnetic system, it can be used for affinity purification, and its recovery is performed easily and quickly with the help of a magnetic field.
