**Meet the editor**

Dr Jamie Davies obtained his PhD from the University of Cambridge and is currently Professor of Experimental Anatomy at the University of Edinburgh. His research investigates how organs develop in a growing human fetus, particularly the processes that enable cells to organize themselves into tissues without needing guidance from outside agencies. His laboratory is also active in

developing ways to combine this basic knowledge with the principles of engineering, with the aim of building new tissues for people who need them. Dr Davies is Editor-in-Chief of the research journal Organogenesis.

Contents

Introductory **Tissue Regeneration – A Clinical**  Chapter **Science Whose Time Has Come 1** 

Chapter 1 **The Role of Physical Factors in Cell Differentiation,** 

Chapter 2 **Effect of Low-Intensity Pulsed** 

**Part 1 Understanding and Manipulating** 

**Endogeneous Healing of Tissues 11** 

**Tissue Repair and Regeneration 13**  Monica Monici and Francesca Cialdai

**Ultrasound on Nerve Repair 35**  Jiamou Li, Hua Zhang and Cong Ren

Chapter 3 **Disinfection of Human Tissues in Orthopedic** 

Peter Diehl, Johannes Schauwecker, Hans Gollwitzer and Wolfram Mittelmeier

Chapter 4 **Heparan Sulfate Proteoglycan Mimetics** 

Chapter 5 **Angiogenesis in Wound Healing 93**  Ricardo José de Mendonça

Chapter 6 **Platelet and Liver Regeneration 109** 

Eleonora Grigoryan

Chapter 7 **Shared Triggering Mechanisms of Retinal Regeneration in Lower Vertebrates and Retinal Rescue in Higher Ones 145** 

**Surgical Oncology by High Hydrostatic Pressure 55** 

**Promote Tissue Regeneration: An Overview 69** 

Johan van Neck, Bastiaan Tuk, Denis Barritault and Miao Tong

Nobuhiro Ohkohchi, Soichiro Murata and Kazuhiro Takahashi

Jamie Davies

### Contents


#### X Contents

### **Part 2 Application of Stem Cells 165**  Chapter 8 **The Therapeutic Potential of Stimulating Endogenous Stem Cell Mobilization 167**  Christian Drapeau, George Eufemio, Paola Mazzoni, Gerhard D. Roth and Susan Strandberg Chapter 9 **Spermatogonial Stem Cells: An Alternate Source of Pluripotent Stem Cells for Regenerative Medicine 203**  Liz Simon, Marie-Claude Hofmann and Paul S. Cooke Chapter 10 **Therapeutic Application of Allogeneic Fetal Membrane-Derived Mesenchymal Stem Cell Transplantation in Regenerative Medicine 221**  Shin Ishikane, Hiroshi Hosoda and Tomoaki Ikeda Chapter 11 **Mesenchymal Stem Cells in CNS Regeneration 237**  Arshak R. Alexanian Chapter 12 **Therapeutic Potential of MSCs in Musculoskeletal Diseases (Osteoarthritis) 261**  José Ramón Lamas, Pilar Tornero-Esteban and Benjamín Fernández-Gutiérrez Chapter 13 **Stem Cell-Mediated Intervertebral Disc Regeneration 283**  Namath S. Hussain, Vickram Tejwani and Mick Perez-Cruet Chapter 14 **Towards Clinical Application of Mesenchymal Stromal Cells: Perspectives and Requirements for Orthopaedic Applications 305**  Marianna Karagianni, Torsten J. Schulze and Karen Bieback Chapter 15 **Oral Tissues as Source for Bone Regeneration in Dental Implantology 325**  Dilaware Khan, Claudia Kleinfeld, Martin Winter and Edda Tobiasch Chapter 16 **Technologies Applied to Stimulate Bone Regeneration 339**  Arnaldo Rodrigues Santos Jr., Christiane Bertachini Lombello and Selma Candelária Genari **Part 3 Use of Scaffolds 367**  Chapter 17 **Preparation of Deproteinized Human Bone and Its Mixtures with Bio-Glass and Tricalcium Phosphate – Innovative Bioactive Materials for Skeletal Tissue Regeneration 369**

Contents VII

Chapter 18 **Endochondral Bone Formation** 

Chapter 19 **Tissue Engineering in Low** 

Abir El-Sadik

Shogo Miyata

Chapter 22 **A Mathematical Model for Wound**

**as Blueprint for Regenerative Medicine 399** 

Peter J. Emans, Marjolein M.J. Caron, Lodewijk W. van Rhijn and Tim J.M. Welting

**Urinary Tract Reconstruction 425**

Chao Feng and Yue-min Xu

**for Tissue Regeneration 453** 

Chapter 21 **Non-Invasive Evaluation Method for Cartilage** 

**Contraction and Angiogenesis 489**  Fred Vermolen and Olmer van Rijn

**Part 4 Modeling and Assessment of Regeneration 471** 

**Tissue Regeneration Using Quantitative-MRI 473** 

Chapter 20 **Novel Promises of Nanotechnology** 

Magdalena Cieslik, Jacek Nocoń, Jan Rauch, Tadeusz Cieslik, Anna Ślósarczyk, Maria Borczuch-Łączka and Aleksander Owczarek


VI Contents

**Part 2 Application of Stem Cells 165** 

Chapter 9 **Spermatogonial Stem Cells:** 

Arshak R. Alexanian

Chapter 12 **Therapeutic Potential of MSCs**

Chapter 13 **Stem Cell-Mediated** 

Chapter 8 **The Therapeutic Potential of Stimulating** 

**Endogenous Stem Cell Mobilization 167**  Christian Drapeau, George Eufemio, Paola Mazzoni,

**Stem Cells for Regenerative Medicine 203**  Liz Simon, Marie-Claude Hofmann and Paul S. Cooke

**Membrane-Derived Mesenchymal Stem Cell Transplantation in Regenerative Medicine 221**  Shin Ishikane, Hiroshi Hosoda and Tomoaki Ikeda

**in Musculoskeletal Diseases (Osteoarthritis) 261**

Namath S. Hussain, Vickram Tejwani and Mick Perez-Cruet

Marianna Karagianni, Torsten J. Schulze and Karen Bieback

Dilaware Khan, Claudia Kleinfeld, Martin Winter and Edda Tobiasch

Arnaldo Rodrigues Santos Jr., Christiane Bertachini Lombello

Chapter 14 **Towards Clinical Application of Mesenchymal Stromal Cells:**

**Regeneration in Dental Implantology 325** 

Chapter 16 **Technologies Applied to Stimulate Bone Regeneration 339** 

Chapter 17 **Preparation of Deproteinized Human Bone and Its Mixtures with Bio-Glass and Tricalcium Phosphate – Innovative Bioactive Materials for Skeletal Tissue Regeneration 369** Magdalena Cieslik, Jacek Nocoń, Jan Rauch, Tadeusz Cieslik, Anna Ślósarczyk, Maria Borczuch-Łączka and Aleksander Owczarek

Chapter 11 **Mesenchymal Stem Cells in CNS Regeneration 237** 

José Ramón Lamas, Pilar Tornero-Esteban and Benjamín Fernández-Gutiérrez

**Intervertebral Disc Regeneration 283**

**Perspectives and Requirements for Orthopaedic Applications 305**

Chapter 15 **Oral Tissues as Source for Bone** 

**Part 3 Use of Scaffolds 367** 

and Selma Candelária Genari

Gerhard D. Roth and Susan Strandberg

**An Alternate Source of Pluripotent** 

Chapter 10 **Therapeutic Application of Allogeneic Fetal** 

	- **Part 4 Modeling and Assessment of Regeneration 471**

**Introductory Chapter** 

Jamie Davies

*UK* 

*University of Edinburgh* 

**Tissue Regeneration – A Clinical** 

**Science Whose Time Has Come** 

Tissue engineering is the application of knowledge gained in the study of basic

The surgically-focused side of the field has a long history, resting mainly on experience with wound healing and *ad-hoc* attempts to improve it. A well-known and long-standing example is modulation of bone healing by the application of physical force that gives rise to the

The more cell biological side of the field is younger because its development had to await the gaining of significant amounts of basic knowledge in molecular cell biology, a field that is only a few decades old. The coming together of cell biology and experimental surgery to drive forward the development of tissue engineering is therefore a relatively recent phenomenon and only in this century has tissue engineering really taken off as a major area

Fig. 1. Rapid growth of tissue engineering as a 21st century discipline. The graph shows the number of publications returned by a Pubmed search for ' *"tissue engineering" <*year*>'*.

developmental cell biology to the construction and repair of human bodies.

image of a patient in traction, so common on humorous 'get well soon' cards.

**1. Introduction** 

of research (Fig 1).
