**3. Results**

*Life Cycle and Development of Diptera*

conditions").

ambient temperature.

**forecast by the IPCC**

**2.7 Statistical analysis**

**according to the provisions of the IPCC**

**2.5 Stabilization of a sample of the population of** *A. aegypti* **from Manaus,** 

**Amazonas, in environmental rooms with temperature and CO2 conditions** 

After the stabilization of the three insectaries in the LEEM, the F28 generation was obtained in the different environmental rooms that started the experiment for post-embryonic development, using three different environments. Rooms 1, 2 and 4 suffer from interference from different temperatures and levels of carbon dioxide, and rooms 2 and 4 refer to 50 and 100 years in front of room 1, which presents

These rooms are equipped with technological devices that guarantee, respectively, the maintenance of ambient temperature, 2.5°C (400 ppm CO2) and 4.5°C (850 ppm CO2) above ambient temperature. The natural conditions of the respective environmental rooms are collected in real time by sensors isolated in the forest and the humidity of all remains constant in relation to the environmental conditions.

**2.6 Biological aspects of** *A. aegypti* **from Manaus, Amazonas, in insectaries kept in environmental rooms that present simulated conditions of climate** 

ing 300 mL of distilled water and 0.055 g of larval food added every 3 days.

the pots and the volume of distilled water was replaced.

After stabilization of insectaries in environmental rooms 1 (ambient temperature), 2 (mild temperature) and 4 (extreme temperature), 125 eggs from each room were counted with the aid of a stereoscopic microscope. These eggs were separated into 5 groups (replicates) of 25 eggs, kept in pots with capacity of 750 mL, contain-

The hatching rate was monitored daily for 19 days under these conditions. The average time of the biological cycle, number of adults (males and females) and larval mortality rate were verified. Exuvia and dead immatures were removed from

Data on the hatching rate of eggs, number of adults (males and females) and mortality rate of immature *A. aegypti* from Londrina, Paraná, were expressed as a percentage. The number of emerged adults was subjected to a Kruskal-Wallis test, at the level of 5% significance, in order to verify differences between the data in the

The results on the number of adults (males and females) and the larval mortality rate of *A. aegypti* from Manaus, Amazonas, were also analyzed by means of percentage. On the other hand, the data regarding the average time of the biological cycle of mosquitoes kept in the different environmental rooms, were analyzed using averages, standard deviation and standard error. At first, these data underwent a Lilliefors normality test (K samples) to find out whether they have a normal distribution or not, and subsequently subjected to Analysis of Variance (ANOVA), followed by Tukey's multiple comparison test (p ≤ 0.05), with the aid of the statistical program SPSS® 14.0 packpage for Windows® (SPSS Inc. 2005 Headquarters, Chicago, Illinois, USA).

temperatures analyzed, using the BioEstat® 5.3 program for Windows [26].

The same process of creating and maintaining the immature and adults of *A. aegypti* from Londrina, Paraná, mentioned in items 2.2 and 2.3, was carried out with a sample of the population of *A. aegypti* from Manaus, Amazonas, collected in the same way on *Campus* I of INPA. However, the blood meal carried out followed the procedure approved by the Ethics Committee on the Use of Animals at INPA (CEUA/INPA: 04/2013-"Breeding of mosquito vectors, under laboratory

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