**5.1 Safety from the environment**

The biofilm gives a safe house and homeostasis to the living beings living inside it, and the imperative segment of this safe house is the extracellular polymeric substance network. This network can possibly forestall the flood of certain antimicrobial operators in this way confining the dissemination of these mixes from the environment into the biofilm.

EPS has appeared to have metal binding property and consequently can sequester lethal metal particles and give defensive functions. In addition to metal binding capacity, the EPS can likewise sequester nutrients and minerals from the environment. This coupling property of EPS is basically because of the nearness of ionizable functional groups, for example, carboxyl, phosphoric, amine, and hydroxyl groups. Researchers found that the sanitized EPS from the container of a freshwater sediment bacterium is fit for restricting copper. Farag detailed the concentration of metals (Ar, Cd, Pb, Hg, and Zn) in various nourishment web segments [20]. Likewise, different authors have announced the stimulatory impact of metal particles on the biofilm development. Researchers in 1997 observed an enlistment of biofilm in the developing colony of *Archaeoglobus fulgidus* when exposed to high grouping of copper and nickel. Bereswill explained the creation of amylovoran: the fundamental polysaccharide of EPS in *Erwinia amylovora*, in the presence of copper [21]. Ordax demonstrated that the EPS removed from *E. amylovora* can bind copper cations and in this manner inferred that the EPS favors the survival of *E. amylovora* under copper pressure [22]. Comparable perceptions of increment in EPS generation within the sight of metal pressure have been accounted for other bacterial species. EPS is additionally known to give a certain level of assurance to the biofilm cells from different natural stresses, for example, UV radiation, pH shifts, osmotic shock, and desiccation.
