**3. Stages in the formation of biofilm**

There are three stages in biofilm formation: initial attachment events, the development of complex biofilms, and separation events by clumps of microorganisms or by a "swarming" phenomenon within the interior of bacterial clusters, bringing about the so-called "seeding dispersal." Once a biofilm has fully formed, it frequently contains diverts in which supplements can flow. Cells in various locales of a biofilm additionally display diverse examples of gene expression. Since biofilms regularly build up their very own metabolism, they are in some cases contrasted with the tissues of higher creatures, in which firmly packed cells cooperate and make a system in which minerals can stream.

The biofilm life cycle is observed in three different stages: attachment, growth of colonies (advancement, and occasional detachment of planktonic cells: Freedrifting, or planktonic microorganisms experience an immersed surface and then within few minutes, they can become attached. They start producing slimy EPS and eventually begin to colonize the surface [1–4]. The formation of EPS allows the biofilm network to develop a three-dimensional and complex structure which is affected by various environmental factors. These complex networks of biofilm structures can be formed within few hours [5]. Biofilms have the sections of cluster of small or large portions of cells. It can also be observed by the process of "seeding dispersal" which helps to discharge the cells which are in singular property. Both the types of cellular separation allow the microbes to get connected either to a surface or to a unique network of biofilm [6, 7] (**Figure 2**).
