**Acknowledgements**

Cancer Institute NSW, Australia Fellowship (MDD). Faculty of Health and Medicine, University of Newcastle, Callaghan, NSW, Australia HDR Scholarship (TG). Imaging from the Hunter Cancer Research Alliance, Hunter Medical Research Institute, Hunter Cancer Biobank (SK). Project support from STRECK Inc.

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provided the original work is properly cited.

© 2018 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/ by/3.0), which permits unrestricted use, distribution, and reproduction in any medium,

*Finding Needles in Haystacks: The Use of Quantitative Proteomics for the Early Detection…*

*DOI: http://dx.doi.org/10.5772/intechopen.80942*

CA 19-9 cancer/carbohydrate antigen 19-9 CEA carcinoembryonic antigen

FIT fecal immunochemical test

ELISA enzyme-linked immunosorbent assay

iTRAQ isobaric tags for relative and absolute quantitation

LC-MS/MS liquid chromatography tandem mass spectrometry

RP-HPLC reversed-phase high performance liquid chromatography

TNM tumor, node, metastases, classification for malignant tumors

Tiffany Gould1,2, Muhammad Fairuz B. Jamaluddin2,3, Joel Petit1,2, Simon J. King1,2,

, Rodney Scott2,3, Peter Pockney1,2 and Matthew D. Dun2,3\*

1 School of Medicine and Public Health, Faculty of Health and Medicine, University

2 Priority Research Centre for Cancer Research, Innovation and Translation, Hunter Medical Research Institute Cancer Research Program, Newcastle, NSW, Australia

3 School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine,

4 Priority Research Centre for Reproductive Science, School of Environmental and

Life Sciences, The University of Newcastle, Callaghan, NSW, Australia

\*Address all correspondence to: matt.dun@newcastle.edu.au

**Acronyms and abbreviations**

CRC colorectal cancer

HSP heat shock protein IHC immunohistochemistry

MS mass spectrometry MMP matrix metalloproteinase MRM multiple reaction monitoring PRM parallel reaction monitoring

TMA tissue microarray TMT tandem mass tag

**Author details**

Brett Nixon4

SRM selected reaction monitoring

of Newcastle, Callaghan, NSW, Australia

University of Newcastle, Callaghan, NSW, Australia

2DE two-dimensional gel electrophoresis

LC liquid chromatography
