*4.1.1.4 Detergent removal*

In the detergent removal method, phospholipids are dissolved in aqueous solution containing detergents at critical micelle concentrations (CMC). Upon detergent removal, the reaction medium frees individual phospholipid molecules that self-assemble into bilayered structures. Detergent removal is mostly achieved by means of a dialysis bag, polystyrene-based absorber beads or Sephadex columns (gel permeation chromatography). Dilution of the resultant mixture with some appropriate aqueous medium leads to restructuration of the formed micelles that evolve to liposomes [4, 6].
