**3. My participation in studies with liposomes**

Forty-four years ago, as an international scholar of the NIH in the Department of Biochemistry of the Health Center of the University of Connecticut, I carried out studies related to the mechanism of stearoyl-CoA desaturase [4]. That is where I first prepared liposomes by sonication of egg lecithin or dimyristoyl lecithin.

Since then I have used liposomes in multiple studies in order to analyze: the exchange of palmitic acid from cytosolic proteins to microsomes, mitochondria, and lipid vesicles [4]; the oleic acid transfer from microsomes to egg lecithin liposomes [5]; the interaction of albumin and fatty-acid-binding protein with membranes: oleic acid dissociation [6]; the removal of fatty acids but not phospholipids from microsomes liposomes and sonicated vesicles by fatty-acid-binding protein [7]; Fe2+- and Fe3+-initiated peroxidation of sonicated and nonsonicated liposomes made of retinal lipids in different aqueous media [8]; lipid peroxidation of membrane phospholipids in the vertebrate retina [9]; the antioxidant properties of melatonin and structural analogues on Fe(2+)-initiated peroxidation of sonicated liposomes made of retinal lipids [10]; the antioxidant behavior of melatonin and structural analogues during lipid peroxidation [11]; and the use of soybean phosphatidylcholine liposomes as model membranes to study lipid peroxidation photoinduced by pterin [12].

### **4. General remarks, conclusions, and perspectives**

It has been fascinating to follow the field of liposomes research during almost five decades. From my experience, it is impossible to predict which aspects in liposomes research will dominate in the future.
