**Procedure**

5 ml of reagent C was added to 1 ml of the test sample. This was thoroughly mixed and left at room temperature for 10 min. Thereafter, 0.5 ml of reagent D was added and allowed to remain at room temperature for 30 min. Absorbance was determined at 620 nm.

Serial dilutions of Bovine serum albumin (Sigma) were treated likewise and used to plot standard graph. The unknown protein value in each test sample is meant to be extrapolated from the standard graph.

**65**

*α-Amylase Production by Toxigenic Strains of* Aspergillus *and* Penicillium

3 g of KI was dissolved in 100 ml of warm distilled water. 0.3 g of Iodine was thereafter added and allowed to dissolve in the solution by mixing and warming.

\* and Victoria Anatolyivna Tsygankova2

1 School of Health Information Management, University College Hospital, Ibadan,

© 2020 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/ by/3.0), which permits unrestricted use, distribution, and reproduction in any medium,

2 Department for Chemistry of Bioactive Nitrogen-Containing Heterocyclic Compounds, Institute of Bioorganic Chemistry and Petrochemistry of the National

Academy of Sciences of Ukraine (NAS), Kyiv, Ukraine

\*Address all correspondence to: ao\_adejuwon@yahoo.ca

provided the original work is properly cited.

*DOI: http://dx.doi.org/10.5772/intechopen.86637*

(ii) Potassium iodide (KI)

**Reagents**

(i) Iodine

**Procedure**

**Author details**

Nigeria

Adekunle Odunayo Adejuwon1
