2.2 Genetic analysis

For genetic analysis, DNA was extracted from young leaves. In the first phase of research, genotyping was performed with 11 SSR loci for variety identification: VVS2 [6]; VVMD5, VVMD7, VVMD27 and VVMD28 [7, 8]; VrZAG62 and VrZAG79 [9]; ISV2, ISV3 and ISV4 [10]; and VMCNG4b9 [11], as described by Ref. [12].

During the second phase of research, genotyping was done with nine microsatellite loci: VVS2 [6]; VVMD5, VVMD7, VVMD25, VVMD27, VVMD28, and VVMD32 [7]; and ssrZAG62 and ssrZAG79 [9] as proposed by the GrapeGen06 consortium and by the European Vitis Database [13].
