**4. Temporal inference of spatial observations obtained on the proleptic axis**

We have tested the possibility of converting spatial observations (along the proleptic axis) into temporal dynamics at a given stage of vegetative or reproductive development.

#### **4.1 Temporal conversion of spatial profiles**

Under controlled and stable environment (25/15°C day/night temperature, VPD 1 kPa, photoperiod 12 h), the development of the proleptic axis of the microvine is stable. The phyllochron is constant reaching ca. 24°C. The growing dynamics of leaves (surface) and berries (volume) from continuous fructification was found to be constant at a given level of phytomer, regardless of the date of bud break [20]. The growth durations of leaves and berries (herbaceous phase) are ca. 220°C after the emission of the phytomer and 500°C after flowering, respectively, as mentioned in Section 2.2. The development of these organs is also spatially stable: the dynamics of leaf area and berry volumes (herbaceous phase) for all levels of phytomer are superimposed when they are represented as a function of cumulative thermal time after the emission of the corresponding phytomer.

Based on these outcomes, the conversion of spatial dynamics of leaf and berry development along the stems into time profiles was tested (**Figure 7**). For this purpose, the positions of the phytomers along the axis were converted into cumulated thermal time after their emission by multiplying their plastochron index (or rank position from the apex) by the phyllochron. The temporal profiles of leaf area and berry volume (green growth phase) resulting from this spatiotemporal conversion are similar to the real temporal profiles obtained at a given level of phytomer [8, 20, 31]. This property makes it possible to reconstruct temporal dynamics of

**Figure 7.**

*Conversion of leaf and young berry growth data collected from the position along the microvine main shoot (plastochron index) into cumulated thermal time after phytomer emergence.*

development from a single spatial observation of the axis at a given stage. The flow of biomass or metabolites within the organs and their responses to environmental constraints were then addressed using those calculated temporal profiles (Section 5.1).
