*4.2.3 Matrigel plug assay*

It is a choice of in vivo angiogenesis assay following administration of gelatinous protein mixture termed Matrigel into subcutaneous space. The target molecules could be administrated with Matrigel at the site of injection and systemically to the circulation system. To precisely elucidate the formation of de novo capillaries, fluorochrome agent could be administrated into the systemic circulation [130].
