*2.1.9 Measuring permeability of cultured cells*

To quantify functional injury to brain capillaries, 14C-sucrose permeability was measured across a monolayer of cerebral capillary endothelial cells [42], under conditions simulating ischemia and reperfusion as described above. Cyclosporin A (1 μM) was used as an inhibitor of the mitochondrial permeability transition [43, 44], to determine if this process is associated with reperfusion injury to the endothelium.
