*2.1.11 Measuring lysis/death of cultured cells*

Lactate dehydrogenase (LDH) release was used to determine cell lysis, and was measured colorimetrically at a wave-length of 490 nm with a commercial kit (Promega). The effectiveness of various antioxidants [19, 20, 45] in preventing cell death was tested by incubating the cells in the presence (1 mM) and absence of glutathione (GSH), N-acetylcysteine (NAC), and gamma-glutamylcysteine (γGlu-Cys).
