**6.1.3 Isolation**

This is a phase that uses selective solid media cast in Petri dishes. The isolation media contains a variety of combination of selective factors (Humbert et al., 1998). *Salmonella* colonies appear as features in their form, color and morphology. Solid media used for isolation are: Rambach medium; Hektoen medium; Salmonella agar - Shigella (SS agar); brilliant green agar and phenol red (VB-RP); xylose lysine Tergitol medium (XLT); Compass mid Salmonella; mannitol lysine, crystal violet, brilliant green; deoxycholate citrate agar lactose sucrose (DCLS); xylose lysine deoxycholate agar (XLD) and Bismuth sulfite agar.

With conventional bacteriological diagnostic methods, other unconventional techniques can be used. These are among others: sensitivity to phage 01, and Felix and Callow and standardized systems (API 20 E, 20 E RAPID, Enterotube Rocks, MIS *Enterobacteriaceae*). The lysis by phage 01 (Felix and Callow) that can be used as a confirmatory test for membership of Salmonella that do not provide positive results with all strains (Gledel and Corbion, 1991).
