**5.1.3.1 Appearance of typical** *Salmonella* **colonies in Brillant green agar and Brillant green sulphadiazine agar**

It is colorless, pink, semi transparent or opaque. The color of medium which surrounds the colony has become pink or red. Some *Salmonella* colonies make semi-transparent green colonies when lactose or sucrose fermentating organisms are present around them. Lactose or sucrose fermantating colonies make yellow to green colonies (*Figure 4).* 

Fig. 4. Appearance of typical *Salmonella* colonies in Brillant green agar

3. At this time, a loop full of the sample is taken and planting to selective agar medium is

1. 1 ml of non selective enrichment medium culture is taken and it is transferred into a tube including 10 ml of selenite cystine. 1 ml is planted into one of 5 tetrathionate

1. Two selective agar medium plate is prepared by drying. One of them may be

2. A loop from every enrichment culture with a diameter of 5 mm is taken and planted to

3. Plates are incubated by reversing at 35-37°C for 24 hours. If typical colonies are not

4. Suspicious two colonies are selected from every selective agar medium and

5. If agar plates are completely full of coliforms, 1/1000 dilution of enrichment culture is

**5.1.3.1 Appearance of typical** *Salmonella* **colonies in Brillant green agar and Brillant green** 

It is colorless, pink, semi transparent or opaque. The color of medium which surrounds the colony has become pink or red. Some *Salmonella* colonies make semi-transparent green colonies when lactose or sucrose fermentating organisms are present around them. Lactose

observed at the end of 24 hours, incubation is extended to 48 hours.

prepared and kept at the room temperature or in the refrigerator. 6. Selective agar plates are kept at 5-8°C until completion of identification tests. Appearances of *Salmonella* colonies in various mediums after incubation are as follows.

or sucrose fermantating colonies make yellow to green colonies (*Figure 4).* 

Fig. 4. Appearance of typical *Salmonella* colonies in Brillant green agar

performed.

**5.1.2 Selectve enrchment** 

medium of 10 ml.

provide single colony.

identification is directed.

**sulphadiazine agar** 

2. Tubes are incubated at 35-37 °C for 24 hours.

enrichment and the other may be selective medium.

**5.1.3 Planting to selective agar medium** 
