**9. References**


Due to an overload of alternative methods and/or formats on the market, food business operators or competent authority, for which microbial analysis of food is only a supporting tool in the assurance of food safety, have difficulties in deciding which method is best fit for

Evolution in alternative rapid methods, mainly immunological and molecular methods, focus on the combination of available techniques e.g. combination of immunocapture and PCR and/or by elaboration of new formats optimizing reading and registration software

This study was supported by the Grant N N312 491340 from the National Science Centre,

Al-Soud, W. A., Ouis I. S., Ljungh, D. Q. Li., Wadström, S. T. (2005). Characterization of the

Al-Soud, W. A.; & Radstrom, P. (2000). Effects of amplification facilitators on diagnostic PCR

Bansal, N. S., Gray, V., & McDonell, F. (2006). Validated PCR assay for the routine detection

Baudart, J., Olaizola, A., Coallier, J., Gauthier V., Laurent P. (2005). Assessment of a new

Beutin, L., Jahn, S., Fach, P. (2009). Evaluation of the 'GeneDisc' real-time PCR system for

associated genes. *Journal of Applied Microbiology.* Vol. 106, pp.1122-1132 Bohaychuk, V.M., Gensler, G.E., King, R.K.,Wu, J.T., McMullen, L.M. (2005). Evaluation of

foodborne pathogens. *Journal Food Protection.* Vol. 68, pp. 2637-2647 Bottari, B., Ercolini, D., Gatti, M., Neviani, E. (2006). Application of FISH technology for

species. *FEMS Immunology & Medical Microbiology* 44:177-182

of *Salmonella* in food. *Journal Food Protection*, Vol. 69, pp. 282-7

*FEMS Microbiology Letters*., Vol. 243,pp. 405-409

*Biotechnology*. Vol. 73, pp. 485-494

Vol. 280, pp. 166-72

pp. 2436-41

PCR inhibitory effect of bile to optimize real-time PCR detection of *Helicobacter* 

in the presence of blood, feces, and meat. *Journal of Clinical Microbiology*, Vol. 38, pp.

technique combining a viability test, whole-cell hybridization and laser-scanning cytometry for the direct counting of viable *Enterobacteriaceae* cells in drinking water.

detection of enterohaemorrhagic *Escherichia coli* (EHEC) O26, O103, O111, O145 and O157 strains according to their virulence markers and their O- and Hantigen-

detection methods for screening meat and poultry products for the presence of

microbiological analysis: current state and prospects. *Applied Microbiology and* 

polymerase chain reaction assay for detecting *Salmonella. Analytical Biochemistry*

of *Salmonella* in foods using real-time PCR. *Journal Food Protection*, Vol.71, No.12,

of *Escherichia coli* O157:H7, *Salmonella* spp. and *Staphylococcus aureus* on fresh,

Chen, W., G. Martinez, and A. Mulchandani. (2000). Molecular beacons: a real-time

Cheng, C. M., Lin, W., Van, K. T., Phan, L., Tran, N. N. & Farmer, D. (2008). Rapid detection

Elizaquivel, P., & Aznar, R. (2008). A multiplex RTi-PCR reaction for simultaneous detection

minimally processed vegetables. *Food Microbiology*, Vol.25, No.5, pp. 705-13

their purpose in their particular context. (Jasson et al., 2010)

**8. Acknowledgment** 

4463-70

Poland.

**9. References** 

rather than introducing new principles of detection or enumeration.


Detection of *Salmonella* spp. Presence in Food 411

Sakai, K., Mori, M., Fujii, A., Iwami, Y., Chukeatirote E., Shirai Y. (2004). Fluorescent *in situ*

Schonenbrucher, V., Mallinson, E.T., Bulte, M. (2008). A comparison of standard cultural

Settanni L, Corsetti A. (2007). The use of multiplex PCR to detect and differentiate food- and

Skowrońska A., Zmysłowska I. (2006). Współczesne metody identyfikacji bakterii stosowane

Szabo, I., Scherer, K., Roesler, U., Appel, B., Nockler, K., Hensel, A., (2008). Comparative

Tomás, D, Rodrigo, A., Hernández, M., Ferrús, M. A. (2009). Validation of Real-Time PCR

spp. in Chicken Feces Samples. *Food Analytical Methods*. Vol2: pp: 180–189 Uyttendaele, M., K., Vanwildemeersch, Debevere, J. (2003). Evaluation of real-time PCR vs

for detection of *Salmonella*. *Letters Applied Microbiology*. Vol. 37 , pp; 386-91 Vieira-Pinto, M., Oliveira, M., Bernardo, F., Martins, C. (2005). Evaluation of Fluorescent in

Vieira-Pinto, M.; Oliveira, M., Bernardo, F., Martins, C. (2007). Rapid detection of *Salmonella* 

Walker, R. L., Kinde, H., Anderson, R. J., Brown, A. E. (2001). Comparison of VIDAS

Wu, V.C.H. (2008). A review of microbial injury and recovery methods in food. *Food* 

Yeh, K. S., C. E. Tsai, S. P. Chen, and C. W. Liao. (2002). Comparison between VIDAS

culture method. *Journal of Food Safety*. Vol. 25, pp: 109–119

*Veterinária e Zootecnia,* Vol.59, No.6, pp.1388-1393

*Microbiology*. Vol. 25, pp: 735-744

*Food Protection*. Vol.72 , No.9, pp.1977-81

(FISH). *Postępy Mikrobiologii*., Vol.45, pp.183-193

Vol.98, pp. 48-56

Vol.69, No.3, pp. 639-43

Vol.69, No.1, pp.1-22

pp. 123-9

microorganisms in an experimental dry sausage during its curing process. *Journal* 

hybridization analysis of open lactic acid fermentation of kitchen refuse using rRNA-targeted oligonucleotide probes. *Journal of Bioscience and Bioengineering*,

methods for the detection of foodborne *Salmonella* species including three new chromogenic plating media. *International Journal of Food Microbiology*. 123, 61e66. Seo, K. H., Valentin-Bon, I. E. & Brackett, R. E. (2006). Detection and enumeration of

*Salmonella* Enteritidis in homemade ice cream associated with an outbreak: comparison of conventional and real-time PCR methods. *Journal Food Protection*,

beverage-associated microorganisms: a review. *Journal of Microbiological Methods*

w ekologii mikroorganizmów wodnych – fluorescencyjna hybrydyzacja *in situ*

examination and validation of ELISA test systems for *Salmonella* typhimurium diagnosis of slaughtering pigs. *International Journal of Food Microbiology*. 124, 65e69.

and Enzyme-Linked Fluorescent Assay-Based Methods for Detection of *Salmonella*

automated ELISA and a conventional culture method using a semi-solid medium

situ Hybridization (FISH) as a rapid screening method for detection of *Salmonella* in tonsils of slaughtered pigs for consumption: a comparison with conventional

sp. in pork samples using fluorescent *in situ* hybridization: a comparison with VIDAS®-SLM system and ISO 6579 cultural method. *Arquivo Brasileiro de Medicina* 

enzyme-linked fluorescent immunoassay using more swab sampling and conventional culture method for *Salmonella* detection in bulk tank milk and in-line milk filters in California dairies. *International Journal of Food Microbiology*. Vol.67,

automatic enzyme-linked fluorescent immunoassay and culture method for

for immobilization and separation of bacterial cells. *Applied and Environmental Microbiology*. Vol.73, pp. 1992-2000


Kumar, R., Surendran, P.K., Thampuran, N. (2008). Evaluation of culture, ELISA and PCR

Maciorowski, K. G., Herrera, P., Jones, F. T.,Pillai, S. D., Ricke, S. C. (2006). Cultural and

Malorny, B, Tassios, P.T., Rådström, P., Cook, N., Wagner, M., Hoorfar, J. (2003b).

*Applied and Environmental Microbiology*, Vol.83, No.1, (May 2003), pp.39-48 Malorny, B., Hoorfar, J., Bunge, C., Helmuth, R. (2003a). Multicenter Validation of the

*and Environmental Microbiology*, Vol. 69, No. 1,(January 2003), pp. 290-296 Malorny, B., Paccassoni, E., Fach, P., Bunge, C., Martin, A., Helmuth, R. (2004). Diagnostic

Manafi, M. (2000). New developments in chromogenic and fluorogenic culture media.

McCarthy, N., Reen, F. J., Buckley, J. F., Frye, J. G., Boyd, E. F. & Gilroy, D. (2009). Sensitive

Moganedi, K. L. M., Goyvaerts, E. M. A., Venter, S. N., Sibara, M. M. (2007). Optimisation of

Oliver J.D. (2005). The viable but nonculturable state in bacteria. *Journal of Microbiology*, 43,

Ootsubo M., Shimizu T., Tanaka R., Sawabe T., Tajima K., Ezura Y. (2003). Seven-hour

Patel, J. R., Bhagwat, A. A., Sanglay, G. C., and Solomon, M. B. (2006). Rapid detection of

Perry, J.D., Freydiere, A.M. (2007). The application of chromogenic media in clinical microbiology. *Journal of Applied. Microbiology.* Vol.103, pp.2046-2055 Piknova , L., Stefanovicova´, A., Drahovska´ , H., Sa´sik, M., & Kuchta, T. (2002). Detection

Pisz, J. M.; Lawrence, J. R.; Schafer, A. N.; Siciliano, S. D. (2007). Differentiation of genes

Priego, R., Medina, L. M., Jordano, R. (2009). Comparison between the Vitek

and Heidelberg. *Journal of Food Protection*, Vol.72, No.11, pp. 2350-7

*International Journal of Food Microbiology*, Vol. 60, pp. 205-18

following pre-cultivation step. *Water SA* 33, 196-202

real-time PCR. *Food Microbiology.* Vol.23, pp.39–46

reaction-based method. *Food Control*, Vol.13, pp.191–194.

*Veterinary Research Communications*, Vol. 30, pp.127-37

*Microbiology*. Vol.73, pp. 1992-2000

*Microbiology,* Vol*.*70, pp.7046-52

93-100

pp.1182–1190

Vol.71, No.3, pp. 312-318

Vol.46, pp. 221-226

for immobilization and separation of bacterial cells. *Applied and Environmental* 

assays for the detection of *Salmonella* in seafood. *Letters. in Applied. Microbiology*.

immunological detection methods for *Salmonella* spp. in animal feeds - A Review.

Standardization of diagnostic PCR for the detection of foodborne pathogens.

Analytical Accuracy of *Salmonella* PCR: towards an International Standard. *Applied* 

real-time PCR for detection of *Salmonella* in food. *Applied and Environmental* 

and rapid molecular detection assays for *Salmonella enterica* Serovars Typhimurium

the PCR-*invA* primers for the detection of *Salmonella* in drinking and surface waters

fluorescence *in situ* hybridization technique for enumeration of *Enterobacteriaceae* in food and environmental water sample. Journal of Applied Microbiology. Vol.95,

*Salmonella* from hydrodynamic pressure-treated poultry using molecular beacon

of *Salmonella* in food, equivalent to ISO 6579, by a three-days polymerase chain

extracted from non-viable versus viable micro-organisms in environmental samples using ethidium monoazide bromide. *Journal of Microbiological Methods*,

Immunodiagnostic Assay System and PCR for the detection of pathogenic

microorganisms in an experimental dry sausage during its curing process. *Journal Food Protection*. Vol.72 , No.9, pp.1977-81


**19** 

Jeongsoon Kim

*South Korea* 

*Mabuk-dong, Giheung-gu, Yongin* 

**Studies on PCR-Based Rapid Detection** 

**1.1 Using molecular biological methods to identified** *Salmonella* **spp.** 

As the industrialization had made food supply to exceed demand, more and more consumers were interested in 'Delicious food' than 'Good quality food' today and they ask for not only taste but also safety. Microbiological safety is one of the most critical factors for ensuring safe food supply. Fig 1. is a flow chart of traditional detection procedure for *Salmonella* spp., showing that it takes about one week. Therefore, a rapid screening method using PCR is the basis of most molecular diagnostic laboratories. As the field of molecular pathology becomes more accessible to practicing pathologists, a working knowledge of PCR techniques is necessary related to biological safety of food is very essential in food industry. *Salmonella* spp. are an important cause of food-borne infections throughout the world, and the availability of rapid and simple detection techniques is critical for the food industry.

Fig. 1. Traditional procedure for the detection of *Salmonella* spp. (ISO/CD draft standard

**1. Introduction** 

6575 revision 2000)

**Systems for** *Salmonella* **spp.** 

*Food Research & Development Center, Samsung Everland Inc.* 

*Salmonella* recovery from pork carcass sponge samples. *Journal of Food Protection.*  Vol. 65, pp. 1656-9

