**5.4.4 Spicer-Edwards H (flagella) test**

These tests may be used instead of polyvalent H test which was specified in 4.4.2. It is used in determination of H antigens.


Isolation and Identification of Salmonellas from Different Samples 155

Specific antibodies occur in serums of those who had typhoid-paratyphoid vaccination and Gruber-Widal reaction is positive in them. If previously vaccinated persons have a pyretic disease, agglutination titration elevates. When vaccinated persons are examined after months, it is seen that they have H agglutinins mainly. Therefore, high H agglutinin titration of those who was vaccinated is meaningless. However, if more than six months has passed from the vaccination date and titration of O agglutination is more than 1/100 and titration

Presence of both O and H agglutinins in persons who had undergone the infection may

Detection of O antibodies in the serum shows that a new infection was experienced. H agglutinins are 1/400 or higher in those who had new infections and they stay for a long time, sometimes for years. Serum titration elevates during an pyretic disease (anamnestic reaction). O agglutinins decrease rapidly in the blood and it may be shown rarely after one

1/100 positive O agglutination and 1/200 positive agglutination are valuable in persons who was not infected and vaccinated. 1/200 O agglutination and 1/400 H agglutination in persons who was infected and vaccinated before is valuable in terms of diagnose of a new infection. Only 1/100-1/200 positive H agglutination shows an undergone infection, new

6 series of tubes are taken, 6 tubes are put in every series as 1/50, 1/100, 1/200, 1/400 serum dilutions, antigen control and serum control. To make serial dilution, 0,5 cm3 salty water except 1st tube and last serum control tube and 0,5 cm3 diluted immunized serum is put into the last tube. Immunized serum which was generally obtained from rabbits as immunized serum is used by diluting 100 or 1000 times and adding 0,5% phenol. First Tube 7.2 cm3 and 0.3 cm3 of saline placed in serum 1 / 25 dilution of each series after the 1st and 2 tube is added 0.5 cm3. Serum was diluted as 1/25 in the 1st tube. Same amount of salty water was

[1] Akman M., Gülmezoglu E. (1976). Tbbi Mikrobiyoloji, 12. Bask Ders Kitab, s: 347–

[4] Ang-Küçüker M., Buget E., Dinçer N., Ang Ö. (1992). İstanbul'da izole edilen *Salmonella* 

[5] Anon, (1988). Salmonellosis Control. The Role of Animal and Product Hygiene. WHO

[6] Anonymous, (1983). Gd Maddeleri Muayene ve Analiz Yöntemleri Kitab. Tarm,

[7] Anonymous, (2005). Mercek Gda Mikrobiyolojisi Uygulamalar. Ed: A.K. Halkman.

[8] Arda, M. (1985). Genel Bakteriyoloji Ankara Üniversitesi Veteriner Fakültesi Yaynlar:402

Orman ve Köy İşleri Bakanlğ Gda İşleri Genel Müdürlüğü, Genel Yayn No:65,

[2] Akman, M. (1977). Bakteri Genetiği Test-Pratik, Cumhuriyet Üniversitesi Yayn No:1

[3] Alkş, N. (1982). Gda Mikrobiyolojisi. Ankara; Yeni İnci Matbaaclk Sanayi

enteritidis suslarnn özellikleri. İnfeks Derg, 6: 91–93.

[9] Arda, M. (2000). Temel Mikrobiyoloji, Ankara: Medisan Yaynevi.

Expert Commitee. Technical Report Series 774.

Başak Matbaaclk Ltd. Şti., 358. Sayfa

elevates in continuous assays, such result is insignificant in the diagnosis.

year. High O titration is not seen with anamnestic reaction.

diluted as much in the 2nd tube and dilution has become 1/50.

vaccination or anamnestic reaction.

**Making the Assay;** 

**6. References** 

352,Ankara.

Özel Yayn No:62-105

last long.


Table 3.
