Seven *Salmonella* spp. strains were tested: *S. typhimurium* (KCTC 2515, KCTC 2412, ATCC 14028), *S. choleraesuis* (KCTC 2929, KCCM 41575), *S. enetritidis* (KCCM 12021), *S. bongori* (KCCM 41758)

Abu, S. M, Piyaunch, B., Teruo, S., Apinya, A., Masaru, W., and Atsushi Y. (2005)

Bassler, H. A., S. J. Flood, K. J. Livak, J. Marmaro, R. Knorr, and C. A. Batt. (1995) Use of a

Bellin, T., M. Pulz, A. Matussek, H. G. Hempen, and F. Gunzer. (2001) Rapid detection of

Bubert, A., I. Hein, M. Rauch, A. Lehner, B. S. Yoon, W. Goebel, and M. Wagner (1999)

Bubert, A., S. Kohler, and W. Goebel (1992) The homologous and heterologous regions

by polymerase chain reaction. Appl. Envir. Microbiol 58: 2625-2632.

Development and assessment of a real-time PCR assay for rapid and sensitive detection of a novel termotolerant bacterium, Lactobacillus thermotolerans, in

fluorogenic probe in a PCR-based assay for the detection of Listeria

enetrohemorrhagic Escherichia coli by real-time PCR with fluorescent

Detection and Differentiation of Listeria spp. by a Single Reaction based on

within the iap gene allow genus- and species-specific identification of Listeria spp.

contamination of foods at a low cost. (Kim *et al*. 2011)

Peanut butter

Egg yolk

Chicken meat

**5. References** 

strains were observed by the iTPA assay using two primer sets and a FRET probe, indicating good specificity. (Table 1)

### **[Detection limits of the iTPA assay]**

The detection limits of the iTPA assay using serial in *S.* Typhimurium strain were determined. KCTC2515 were determined and the lowest number of cells detected was 4 x 101 CFU per iTPA reaction (Table 2).

