**5.4.2 Polyvalent H (flagella) antigen agglutination test**


Antiserums which are used in serological tests of *Salmonellas* can be classified as follows. Pure *Salmonellas* uspicious culture reproduced in TSI agar, agar agar or bouillon and culture of *Salmonellas* erotypes reproduced in normal or oblique agar can be classified as follows to

a. *Salmonella* polyvalent 0 (somatic) antiserum: 1,16,19,22,23,24,25 and vi in the least, b. *Salmonella* individual (0) somatic group anti-serums: A,B,C1,C2,D E (E1,E2,E3,E4),

c. *Salmonella* polyvalent H (flagella) antiserum: It will include agglutinins of a,b,c,d,en,en,enx,fg,tgt,gm,gms,gp,gpu,gq,gst,gt,i,k,lv,lw,lz13,lz28,mt,r,y,z,z4z23,z4z24,

d1) *Salmonella* H antiserum Spicer –Edwards 3: Gives reaction with a, d, eh, k, z, z4z23,

d2) *Salmonella* H antiserum Spicer –Edward 4: Gives reaction with b, d, fg, fgt, gm, gms,

d3) *Salmonella* H antiserum e,n complex: It gives reaction with enx and enz 15

d4) *Salmonella* H antiserum L complex: Gives reaction with 1v,1w,1zl3,lz28 antigens. d5) *Salmonella* H antiserum 1 complex: Gives reaction with 1,2; 1,5 1,6 1,7 ve z6 antigens.

1. First, reliability of antiserum should be detected. For this, various dilutions of the

3. A small amount of culture a taken and put on both parts of the lame. (cultures in

4. One drop each from 0,85% sodium chloride solution is put on drops above and it is

5. One drop of *Salmonella* polyvalent (0) antiserum is put into the first section and mixed

6. The mixture is mixed by moving to the front and back for one minute and it is

1. Various dilutions of antiserum is prepared and it is controlled that whether the

2. 5 ml of 24 hours H bouillon culture is taken, 5 ml of 0,6% formalin physiological saline is added on it. It is kept for one hour. Formalin bouillon is kept for a couple of days at 5

3. A small serological tube (10 X 75 mm or 13X 100 mm) is taken. 0,02 ml (one drop) is put from H (flagella) antiserum which was diluted appropriately and 1 ml of formalin

**5.4 Serologucal tests** 

be used in serological tests:

z4z32,z6,z10,z29,1,2,1,5,1,6 ve 1,7 antigens. d. *Salmonellas* picer-Edward's H (flagella) antiserums:

**5.4.1 Lame test for polyvalent 0 (somatic) antigen** 

2. The lame is divided into two by a glass cutter.

**5.4.2 Polyvalent H (flagella) antigen agglutination test** 

antiserum is safe with a known culture.

bouillon culture (antigen) is added on it.

z4z32 ve z29 antigens.

antibodies.

emulsified well.

to 8°C if required.

well.

It is consisted of seven antiserums and it gives a reaction as follows:

gmt, gp, gpu, gq, gst, ms, mt, k, r, z, ve z10 antigens.

antiserum should ne prepared and controlled with a certain culture.

normal oblique agar and 24 hours cultures of TSI agar should be used.).

examined on a dark base. Positive reaction is rapid and strong reaction.

F,G,H,I, vi,

