2.2 Body composition measuring method

Body composition measurement was done by multisegmental bioelectrical impedance analysis (BIA). We relied on a professional measurement equipment— In Body 720 Tetrapolar 8-Point Tactile Electrode System (Biospace, Co., Ltd.) and DSM-BIA method (direct segmental multi-frequency bioelectrical impedance analysis). BIA is a widely used standard method for determining whole body composition and segmental lean mass measurements. InBody [45] body composition analyzer has high test-pretest reliability and accuracy (ICC 0.9995) [46]. Compared with DXA as a golden standard, the interclass correlation coefficient of BIA was between 0.96 and 0.99 in the normal-weight population [47]. All measurement had been performed between 2013 and 2016, in the morning hours. The procedure and course of analysis have been described in previous studies [33, 48].
