**Abstract**

 Vital tooth-derived demineralized dentin matrix (DDM) has a bone-inductive ability, while non-vital tooth-derived DDM lost it. Acid treatment for dentin provides the increase of surface area, the release of matrix-binding growth factors such as BMPs, and the decrease of the infection risk. Human autograft of vital toothderived DDM was achieved first in Japan 2002, while first bone autograft was noted in Italy 1820. This paper introduced dentin/bone biology and a unique clinical case, combined with two types of non-vital tooth-derived DDM (roots, granules) for lateral bone augmentation. A 63-year-old woman revealed highly atrophic mandible in 2015. Three non-vital teeth were extracted, changed in shape, demineralized in 2% HNO3, were rinsed, and were grafted immediately. The CT images at 3 months after the graft showed remarkable lateral augmentation. DDM scaffolds were received to host, and two fixtures were placed into the DDM-augmented bone. The patient was successfully restored with their own DDM scaffolds and implant surgery.

**Keywords:** dentin, bone, material, scaffold

## **1. Introduction**

Regenerative medicine is based on applied biomaterial science. Biomaterials have a strong impact on the patient cure for improving the quality of life. Recently, absorbable materials have been needed in the field of tissue engineering. We have been challenging to develop bioabsorbable dentin scaffolds [1–13], harmonized with bone remodeling, by using the ultrasonic acid-etching technology [14–17] (**Figure 1**).
