**2.1 2D culture of ADSC and hACh**

ADSC (PT-5006, Lonza) were cultured in 175 cm<sup>2</sup> T-flasks (<6th passage) in Adipose-Derived Stem Cell Basal Medium (ADSC-BM) (PT-3273, Lonza) supplemented with Adipose-Derived Stem Cell Growth Medium (ADSC-GM) SingleQuots (PT-4503, Lonza). hACh cells (CC-2550, Lonza) were cultured at 10,000 cells/cm<sup>2</sup> from passages 2–6 in 25, 75, and 175 cm2 T flasks. The growth medium consisted of Chondrocyte Basal Medium (CBM) (CC-3217, Lonza) plus SingleQuots of Growth Supplements (CC-4409, Lonza) containing R3-IGF-1, bFGF, transferrin, insulin, FBS, and gentamicin/amphotericin-B. Cultures were maintained in the incubator in humidified atmosphere at 37°C and 5% CO2.
