*2.1.1.1.1 SS-OCT and SS-OCTA features*

The corresponding SS-OCT scan of the right eye showed marked thickening of the choroid with secondary undulations of the overlying RPE layer. The subretinal space showed multiple loculi of serous fluid and complete disruption of the

**89**

**Figure 1.**

*Swept-Source Optical Coherence Tomography and Optical Coherence Tomography Angiography…*

ellipsoid zone. SS-OCTA revealed loss of the normal hyperintense homogenous texture of the choriocapillaris and the development of moth-eaten like hypointense areas. SS-OCT examination of the left eye revealed large sub-foveal hyperreflective

*within the lesion denote a predominantly inactive CNV complex.*

*(A) Top left: Color fundus photo and FFA of the right eye of a 58-year-old man presenting with acute attack of VKH. The posterior pole has a creamy yellowish appearance due to combination of multifocal neurosensory detachment and sub-retinal deposition of serous exudates. The ONH is hyperemic. FFA shows patchy choroidal filling persisting well into the venous phase, along with multiple pin-point leakage at the level of the RPE and diffuse hyperfluorescence due to pooling of dye into localized areas of neurosensory detachment. Bottom left: Radial scan SS-OCT shows marked increase in choroidal thickening (800 μm) and engorged choroidal vessels. Note the difficult delineation of the choroidal-scleral interface (CSI) due to optical hyporeflectivity secondary to engorged choroid. There are innumerable hyperreflective dots scattered within the choroid and possibly represent cellular inflammatory aggregates. The RPE shows multiple folds (white arrows). The neurosensory retina shows multiple hyporeflective loci separated by hyperreflective tissue septa (asterisks). Central macular thickness (CMT) is 970 μm. The hyporeflective loci are filled with hyperreflective amorphous material. There is complete disruption of the ellipsoid zone and outer retinal layers. Right: En-face SS-OCTA image of the choriocapillaris in a 6 × 6 mm field. Note the multiple discrete hypointense flow voids that are scattered within the choriocapillaris layer (white circles). The corresponding flow-density map shows blue color shades corresponding to the flow-void areas and denoting marked reduction of vessel density. (B) Radial scan SS-OCT images of the same patient at 15 days and 4 months after high-dose systemic steroid treatment. Note complete resolution of neurosensory detachment, and restoration of the ellipsoid zone. There is residual thickening of the RPE. Note improved delineation of the CSI and progressive disappearance of the previously seen hyperreflective dots in the choroid with resolution of choroidal inflammation. Bottom right: En-face SS-OCTA image of the choriocapillaris in a 6 × 6 mm field and the corresponding flow-density map at 4-month follow-up visit show significant recovery of the normal texture of the choriocapillaris denoting improvement of choriocapillaris perfusion. (C) Left: Color fundus photo and FFA of the left eye of the same patient. In addition to the features of acute stage of VKH, the macular area shows sub-retinal greenish-yellow lesion that demonstrates early minimal leakage on FFA and late staining suggestive of its predominant scar component. Right: Radial scan SS-OCT shows choroidal thickening (669 μm), blurred CSI, multiple neurosensory detachments with subretinal hyporeflective foci and hyperreflective amorphous deposits. Note the hyperreflective fusiform sub-foveal lesion. (D) Left: Radial scans SS-OCT during follow-up visits show resolution of the multifocal neurosensory detachment with persistence of the previously noted sub-retinal amorphous lesion. There is resolution of choroidal inflammation and improved visualization of the CSI. Bottom right: En-face SS-OCTA image and the corresponding flow-density map of the outer retina in a 6 × 6 mm field at 4-month follow-up visit show the hyperintense signal corresponding to high flow within neovascular complex. The abundant large mature vessels* 

*DOI: http://dx.doi.org/10.5772/intechopen.84245*

*Swept-Source Optical Coherence Tomography and Optical Coherence Tomography Angiography… DOI: http://dx.doi.org/10.5772/intechopen.84245*

#### **Figure 1.**

*A Practical Guide to Clinical Application of OCT in Ophthalmology*

*2.1.1.1 Case presentation*

[29, 30]. These areas of choriocapillaris loss appear on OCTA examination as sharply demarcated flow-voids that might recover after resolution of inflammation [31, 32]. Recurrent attacks of inflammation with subsequent breaching of the RPE-Bruch's complex could trigger CNV formation. In that context OCTA helps differentiating CNV from inflammatory tissue by demonstrating the hyperintense

A 58-year-old male presenting with bilateral diminution of vision of approximately 3 months duration. The patient reported more severe drop of vision in the left eye. He had history of previous similar attacks of visual disturbances in both eyes. His best-corrected visual acuity (BCVA) was 6/60 and 1/60 in the right and left eyes, respectively. Fundus examination of the right eye revealed multifocal serous retinal detachment in the posterior pole along with extensive sub-retinal exudate formation and optic nerve head (ONH) hyperemia. FFA revealed multiple leaking points at the level of the RPE with pooling of dye into the sub-retinal space and hyperfluorescence of the ONH due to leakage from dilated optic disc vessels. Fundus examination of the left eye showed similar findings in the posterior pole in addition to a sub-macular greenish-yellow lesion that demonstrated early hyperfluorescence with minimal

The corresponding SS-OCT scan of the right eye showed marked thickening of the choroid with secondary undulations of the overlying RPE layer. The subretinal space showed multiple loculi of serous fluid and complete disruption of the

signal characteristic of CNV formation in the outer retina slab [3, 4].

leakage and late staining suggestive of predominantly scarred CNV.

*2.1.1.1.1 SS-OCT and SS-OCTA features*

**88**

*(A) Top left: Color fundus photo and FFA of the right eye of a 58-year-old man presenting with acute attack of VKH. The posterior pole has a creamy yellowish appearance due to combination of multifocal neurosensory detachment and sub-retinal deposition of serous exudates. The ONH is hyperemic. FFA shows patchy choroidal filling persisting well into the venous phase, along with multiple pin-point leakage at the level of the RPE and diffuse hyperfluorescence due to pooling of dye into localized areas of neurosensory detachment. Bottom left: Radial scan SS-OCT shows marked increase in choroidal thickening (800 μm) and engorged choroidal vessels. Note the difficult delineation of the choroidal-scleral interface (CSI) due to optical hyporeflectivity secondary to engorged choroid. There are innumerable hyperreflective dots scattered within the choroid and possibly represent cellular inflammatory aggregates. The RPE shows multiple folds (white arrows). The neurosensory retina shows multiple hyporeflective loci separated by hyperreflective tissue septa (asterisks). Central macular thickness (CMT) is 970 μm. The hyporeflective loci are filled with hyperreflective amorphous material. There is complete disruption of the ellipsoid zone and outer retinal layers. Right: En-face SS-OCTA image of the choriocapillaris in a 6 × 6 mm field. Note the multiple discrete hypointense flow voids that are scattered within the choriocapillaris layer (white circles). The corresponding flow-density map shows blue color shades corresponding to the flow-void areas and denoting marked reduction of vessel density. (B) Radial scan SS-OCT images of the same patient at 15 days and 4 months after high-dose systemic steroid treatment. Note complete resolution of neurosensory detachment, and restoration of the ellipsoid zone. There is residual thickening of the RPE. Note improved delineation of the CSI and progressive disappearance of the previously seen hyperreflective dots in the choroid with resolution of choroidal inflammation. Bottom right: En-face SS-OCTA image of the choriocapillaris in a 6 × 6 mm field and the corresponding flow-density map at 4-month follow-up visit show significant recovery of the normal texture of the choriocapillaris denoting improvement of choriocapillaris perfusion. (C) Left: Color fundus photo and FFA of the left eye of the same patient. In addition to the features of acute stage of VKH, the macular area shows sub-retinal greenish-yellow lesion that demonstrates early minimal leakage on FFA and late staining suggestive of its predominant scar component. Right: Radial scan SS-OCT shows choroidal thickening (669 μm), blurred CSI, multiple neurosensory detachments with subretinal hyporeflective foci and hyperreflective amorphous deposits. Note the hyperreflective fusiform sub-foveal lesion. (D) Left: Radial scans SS-OCT during follow-up visits show resolution of the multifocal neurosensory detachment with persistence of the previously noted sub-retinal amorphous lesion. There is resolution of choroidal inflammation and improved visualization of the CSI. Bottom right: En-face SS-OCTA image and the corresponding flow-density map of the outer retina in a 6 × 6 mm field at 4-month follow-up visit show the hyperintense signal corresponding to high flow within neovascular complex. The abundant large mature vessels within the lesion denote a predominantly inactive CNV complex.*

ellipsoid zone. SS-OCTA revealed loss of the normal hyperintense homogenous texture of the choriocapillaris and the development of moth-eaten like hypointense areas. SS-OCT examination of the left eye revealed large sub-foveal hyperreflective

lesion with complete disorganization of the outer retinal layers and minimal overlying subretinal fluid, reminiscent of inactive type 2 CNV. SS-OCTA examination of the left eye confirmed inactive CNV formation. After receiving systemic steroid treatment the patient had complete resolution of the acute attack, with restoration of the normal retinal architecture and normal choroidal thickness in the right eye. BCVA in the right eye improved to 6/6 (**Figure 1(A)–(D)**).

#### *2.1.1.2 Case presentation*

A 16-year-old female presenting with bilateral diminution of vision of approximately 2 weeks duration. Her BCVA was 6/60 bilaterally. Fundus examination of both eyes revealed multifocal serous retinal detachment in the posterior pole along with yellowish sub-retinal exudate formation and ONH hyperemia. FFA revealed numerous leaking points at the level of the RPE with pooling of dye into the sub-retinal space and hyperfluorescence of the ONH due to leakage from dilated optic disc vessels. Five months after the patient received high-dose steroid therapy, the fundus of both eyes showed resolution of the previously noted neurosensory detachments and development of diffuse RPE and choroidal pigmentary disturbances. The macular area of the left eye showed a sub-retinal greenish lesion suggestive of CNV formation.

#### *2.1.1.2.1 SS-OCT and SS-OCTA features*

The corresponding SS-OCT scan of the right eye in the chronic stage showed residual neurosensory detachment with persistent thickening of the choroid. SS-OCT of the left eye showed sub-retinal hyperreflective amorphous lesion corresponding to the macular lesion seen in the color photo. SS-OCT was inconclusive in differentiating the sub-retinal inflammatory material from CNV due to similar optical reflectance properties of both lesions. On the other hand, SS-OCTA of the left eye decisively excluded CNV formation (**Figure 2(A)** and **(B)**).

#### *2.1.2 Serpiginous choroiditis*

Serpiginous choroiditis is an autoimmune disorder characterized by primary choriocapillaropathy in the form of progressive vascular occlusion of the choriocapillaris and possibly larger choroidal vessels with secondary ischemic damage of the RPE and neurosensory retina. The classic presentation of the acute form is single or multiple sub-retinal creamy-white lesions developing at the edge of the optic disc and wind in the posterior pole in a centripetal serpentine or helical pattern. Less typical variants of the disease that do not have a peripapillary component include macular serpiginous, and ampiginous choroiditis. The latter form has an initial benign clinical presentation reminiscent of acute posterior multifocal placoid pigment epitheliopathy, and later follows a relentless progressive course. Serpiginous choroiditis is bilateral with propensity to recurrence. Disease re-activation is characterized by development of newer lesions at the edges of old healed scars. Chronic stage is characterized by diffuse atrophy and disappearance of the choriocapillaris with subsequent secondary atrophy of the RPE and outer retina, scarring and RPE pigmentary disturbances [33, 34]. The resultant choroidal thinning and loss of choriocapillaris will cause alteration of the normal structural OCT features in the form of forward bowing of the choroid with loss of the normal bowl-shaped configuration and anterior displacement of the Sattler's vessel layer, hence development of diffuse irregular flow-voids and enhanced visualization of the medium and largesized choroidal vessels on OCTA slab of the choriocapillaris [35–37]. The disease

**91**

**Figure 2.**

*2.1.2.1 Case presentation*

*Swept-Source Optical Coherence Tomography and Optical Coherence Tomography Angiography…*

is notorious of aggressive course with propensity to involve the macular area and subsequent profound vision loss, which prompts briskly intervention with high-dose

*(A) Color fundus photo and FFA of both eyes of a 16-year-old female with acute attack of VKH shows ONH hyperemia, and multiple neurosensory detachments with yellowish subretinal exudates. FFA demonstrates bilateral numerous leaking points at the level of the RPE. Note the classic starry-sky appearance that is characteristic of acute VKH. Later frames of FFA show pooling of dye into areas of multifocal neurosensory detachment, and ONH hyperfluorescence. (B) Top: Composite fundus photo of both eyes of the same patient 5 months later. The patient received high-dose systemic steroid treatment and developed chronic stage of VKH. Note the characteristic 'sunset glow' appearance of the fundus. Formation of Dalen-Fuchs nodules is more evident in the left eye (asterisk). Note the greenish sub-retinal lesion in the macular area in the left eye. Bottom left: High-definition line scan (12.0 mm) SS-OCT of the right eye shows irregular thickening of the RPE layer, sub-RPE and sub-retinal hyperreflective deposits, and residual neurosensory detachment. Note that the choroid is still grossly thickened. Bottom right: High-definition line scan (12.0 mm) SS-OCT of the left eye. Note the sub-foveal hyperreflective amorphous lesion corresponding to the macular lesion seen in the color photo. In this situation SS-OCT was inconclusive in excluding CNV formation. The corresponding en-face SS-OCTA image of the outer retina in a 6 × 6 mm field demonstrated* 

A 28-year-old female patient who was a known case of bilateral serpiginous choroiditis. The patient presented with recent complaint of drop of vision approximately 1 month ago. Fundus examination in both eyes revealed large sharply circumscribed areas of sub-retinal fibrosis, with dense RPE clumps formation and

steroid therapy or other immunosuppressive agents for vision salvage [33].

*normal hypointense appearance of an avascular outer retina, and decisively excluded CNV formation.*

*DOI: http://dx.doi.org/10.5772/intechopen.84245*

*Swept-Source Optical Coherence Tomography and Optical Coherence Tomography Angiography… DOI: http://dx.doi.org/10.5772/intechopen.84245*

#### **Figure 2.**

*A Practical Guide to Clinical Application of OCT in Ophthalmology*

BCVA in the right eye improved to 6/6 (**Figure 1(A)–(D)**).

*2.1.1.2 Case presentation*

suggestive of CNV formation.

*2.1.2 Serpiginous choroiditis*

*2.1.1.2.1 SS-OCT and SS-OCTA features*

lesion with complete disorganization of the outer retinal layers and minimal overlying subretinal fluid, reminiscent of inactive type 2 CNV. SS-OCTA examination of the left eye confirmed inactive CNV formation. After receiving systemic steroid treatment the patient had complete resolution of the acute attack, with restoration of the normal retinal architecture and normal choroidal thickness in the right eye.

A 16-year-old female presenting with bilateral diminution of vision of approxi-

The corresponding SS-OCT scan of the right eye in the chronic stage showed residual neurosensory detachment with persistent thickening of the choroid. SS-OCT of the left eye showed sub-retinal hyperreflective amorphous lesion corresponding to the macular lesion seen in the color photo. SS-OCT was inconclusive in differentiating the sub-retinal inflammatory material from CNV due to similar optical reflectance properties of both lesions. On the other hand, SS-OCTA of the

Serpiginous choroiditis is an autoimmune disorder characterized by primary choriocapillaropathy in the form of progressive vascular occlusion of the choriocapillaris and possibly larger choroidal vessels with secondary ischemic damage of the RPE and neurosensory retina. The classic presentation of the acute form is single or multiple sub-retinal creamy-white lesions developing at the edge of the optic disc and wind in the posterior pole in a centripetal serpentine or helical pattern. Less typical variants of the disease that do not have a peripapillary component include macular serpiginous, and ampiginous choroiditis. The latter form has an initial benign clinical presentation reminiscent of acute posterior multifocal placoid pigment epitheliopathy, and later follows a relentless progressive course. Serpiginous choroiditis is bilateral with propensity to recurrence. Disease re-activation is characterized by development of newer lesions at the edges of old healed scars. Chronic stage is characterized by diffuse atrophy and disappearance of the choriocapillaris with subsequent secondary atrophy of the RPE and outer retina, scarring and RPE pigmentary disturbances [33, 34]. The resultant choroidal thinning and loss of choriocapillaris will cause alteration of the normal structural OCT features in the form of forward bowing of the choroid with loss of the normal bowl-shaped configuration and anterior displacement of the Sattler's vessel layer, hence development of diffuse irregular flow-voids and enhanced visualization of the medium and largesized choroidal vessels on OCTA slab of the choriocapillaris [35–37]. The disease

left eye decisively excluded CNV formation (**Figure 2(A)** and **(B)**).

mately 2 weeks duration. Her BCVA was 6/60 bilaterally. Fundus examination of both eyes revealed multifocal serous retinal detachment in the posterior pole along with yellowish sub-retinal exudate formation and ONH hyperemia. FFA revealed numerous leaking points at the level of the RPE with pooling of dye into the sub-retinal space and hyperfluorescence of the ONH due to leakage from dilated optic disc vessels. Five months after the patient received high-dose steroid therapy, the fundus of both eyes showed resolution of the previously noted neurosensory detachments and development of diffuse RPE and choroidal pigmentary disturbances. The macular area of the left eye showed a sub-retinal greenish lesion

**90**

*(A) Color fundus photo and FFA of both eyes of a 16-year-old female with acute attack of VKH shows ONH hyperemia, and multiple neurosensory detachments with yellowish subretinal exudates. FFA demonstrates bilateral numerous leaking points at the level of the RPE. Note the classic starry-sky appearance that is characteristic of acute VKH. Later frames of FFA show pooling of dye into areas of multifocal neurosensory detachment, and ONH hyperfluorescence. (B) Top: Composite fundus photo of both eyes of the same patient 5 months later. The patient received high-dose systemic steroid treatment and developed chronic stage of VKH. Note the characteristic 'sunset glow' appearance of the fundus. Formation of Dalen-Fuchs nodules is more evident in the left eye (asterisk). Note the greenish sub-retinal lesion in the macular area in the left eye. Bottom left: High-definition line scan (12.0 mm) SS-OCT of the right eye shows irregular thickening of the RPE layer, sub-RPE and sub-retinal hyperreflective deposits, and residual neurosensory detachment. Note that the choroid is still grossly thickened. Bottom right: High-definition line scan (12.0 mm) SS-OCT of the left eye. Note the sub-foveal hyperreflective amorphous lesion corresponding to the macular lesion seen in the color photo. In this situation SS-OCT was inconclusive in excluding CNV formation. The corresponding en-face SS-OCTA image of the outer retina in a 6 × 6 mm field demonstrated normal hypointense appearance of an avascular outer retina, and decisively excluded CNV formation.*

is notorious of aggressive course with propensity to involve the macular area and subsequent profound vision loss, which prompts briskly intervention with high-dose steroid therapy or other immunosuppressive agents for vision salvage [33].

#### *2.1.2.1 Case presentation*

A 28-year-old female patient who was a known case of bilateral serpiginous choroiditis. The patient presented with recent complaint of drop of vision approximately 1 month ago. Fundus examination in both eyes revealed large sharply circumscribed areas of sub-retinal fibrosis, with dense RPE clumps formation and variable degree of RPE pigmentary disturbances. Some of the lesions have coalesced together forming a continuum of sub-retinal scarring that involved almost the entire posterior pole. The distribution of the lesions was suggestive of previous episodes of classic form of serpiginous choroiditis. BCVA was counting fingers (CF) at 50 cm and 3/60 in the right and left eyes, respectively.

#### *2.1.2.1.1 SS-OCT and SS-OCTA features*

SS-OCT in both eyes showed marked thinning of the choroid with loss of the normal bowl-shaped configuration due to severe erosion of the choriocapillaris. The outer retina showed marked disorganization and atrophy. The RPE-Bruch's complex, external limiting membrane layer (ELM) and inner segment-outer segment photoreceptors junction (IS/OS) were replaced by amorphous hyperreflective material. SS-OCTA showed irregular hypointense areas of flow-void amidst areas of preserved choriocapillaris denoting patchy loss of choriocapillaris (**Figure 3(A)** and **(B)**).

#### *2.1.2.2 Case presentation*

A 37-year-old female patient who was a known case of bilateral serpiginous choroiditis presenting for routine follow-up. BCVA was 6/60 and 6/36 in the right and left eyes, respectively. The posterior pole in both eyes showed large wellcircumscribed areas of extensive chorioretinal atrophy involving the peripapillary area and extending into the macular region. The retinal layers and even underlying choroidal vasculature have virtually disappeared from wide areas of the lesion with unveiling of the underlying sclera.

#### *2.1.2.2.1 SS-OCT and SS-OCTA features*

SS-OCT in both eyes showed marked thinning of the fovea, and marked disorganization and atrophy of the outer retinal layers. The choroid showed diffuse loss of the choriocapillaris and of the larger choroidal vessels with subsequent loss of the normal bowl-shaped configuration. SS-OCTA showed diffuse loss of the choriocapillaris with unveiling of the larger choroidal vessels (**Figure 4(A)** and **(B)**).

#### *2.1.3 Multifocal choroiditis (MFC) and punctate inner choroidopathy (PIC)*

MFC and PIC are idiopathic chorioretinal inflammatory disorders that are grouped under the spectrum of white dot syndromes. The phenotypic features of both entities overlap in many aspects to the extent that they are considered variants of the same disorder rather than separate clinical entities. MFC/PIC have predilection for young myopic females. Their hallmark is the development of yellowishwhite chorioretinal lesions scattered throughout the posterior pole and the periphery of the fundus. Histologically, these lesions are composed of aggregates of inflammatory cells beneath the RPE that cause multifocal conical elevation of the RPE cell layer in the form of inflammatory PED. These lesions eventually breakthrough the RPE into the sub-retinal space with subsequent release of the inflammatory infiltrates into the outer retina. Recurrent episodes of inflammation cause structural retinal damage. The chorioretinal pathologic cascade is associated with vitritis that is most marked in cases of MFC compared to PIC. As chronicity ensues these lesions develop into punched-out chorioretinal scars [38–41]. Secondary CNV formation is a frequent complication, mounting up to one-third of cases, though some studies report much higher incidence [42–44]. CNV superimposed

**93**

**Figure 3.**

inflammation [10].

*Swept-Source Optical Coherence Tomography and Optical Coherence Tomography Angiography…*

on MFC/PIC is usually a type 2 variant and is notoriously difficult to detect or to follow-up its response to anti-vascular endothelial growth factor (anti-VEGF) agents using conventional angiography techniques or structural OCT because it is characterized by minimal leakage on angiography and fluid accumulation on OCT, in addition to overlapping angiographic and OCT features with MFC/PIC-induced

*to combination of compensatory dilatation and anterior displacement of Sattler's vessel layer.*

*(A) Top: Color fundus photo and FFA of both eyes of a 28-year-old female in chronic stage of serpiginous choroiditis. Note the diffuse plaques of sub-retinal fibrosis in the peripapillary area that radiate to involve almost the entire posterior pole. RPE pigment clumps formation is seen in the center of these lesions. On the corresponding FFA, these lesions show central blocked fluorescence surrounded by hyperfluorescent rim due to staining of the scar tissue. Bottom: Corresponding horizontal line scan (9.0 mm) SS-OCT (right), and vertical line scan (9.0 mm) SS-OCT (left) of both eyes show marked thinning of the choroid (255 and 240 μm in the right and left eyes, respectively). Note the irregular choroidal contour, marked thinning of the choriocapillaris and enhanced visualization of larger-sized choroidal vessels. The RPE-Bruch's complex, ellipsoid zone, ELM and IS/OS could not be identified and are replaced by amorphous hyper-reflective deposits. Note the foveal thinning and marked disorganization of the overlying neurosensory retina. (B) En-face SS-OCTA image and the corresponding flow density map of the choriocapillaris of the right eye in a 3 × 3 mm field. There are scattered sharply circumscribed areas of flow-voids due to loss of choriocapillaris (white arrows) with corresponding blue shades on the flow-density map. Note the enhanced visualization of the hyperintense signal of the larger choroidal vessels at the edges of the flow-voids and elsewhere due* 

*DOI: http://dx.doi.org/10.5772/intechopen.84245*

*Swept-Source Optical Coherence Tomography and Optical Coherence Tomography Angiography… DOI: http://dx.doi.org/10.5772/intechopen.84245*

#### **Figure 3.**

*A Practical Guide to Clinical Application of OCT in Ophthalmology*

at 50 cm and 3/60 in the right and left eyes, respectively.

*2.1.2.1.1 SS-OCT and SS-OCTA features*

(**Figure 3(A)** and **(B)**).

*2.1.2.2 Case presentation*

unveiling of the underlying sclera.

*2.1.2.2.1 SS-OCT and SS-OCTA features*

variable degree of RPE pigmentary disturbances. Some of the lesions have coalesced together forming a continuum of sub-retinal scarring that involved almost the entire posterior pole. The distribution of the lesions was suggestive of previous episodes of classic form of serpiginous choroiditis. BCVA was counting fingers (CF)

SS-OCT in both eyes showed marked thinning of the choroid with loss of the normal bowl-shaped configuration due to severe erosion of the choriocapillaris. The outer retina showed marked disorganization and atrophy. The RPE-Bruch's complex, external limiting membrane layer (ELM) and inner segment-outer segment photoreceptors junction (IS/OS) were replaced by amorphous hyperreflective material. SS-OCTA showed irregular hypointense areas of flow-void amidst areas of preserved choriocapillaris denoting patchy loss of choriocapillaris

A 37-year-old female patient who was a known case of bilateral serpiginous choroiditis presenting for routine follow-up. BCVA was 6/60 and 6/36 in the right and left eyes, respectively. The posterior pole in both eyes showed large wellcircumscribed areas of extensive chorioretinal atrophy involving the peripapillary area and extending into the macular region. The retinal layers and even underlying choroidal vasculature have virtually disappeared from wide areas of the lesion with

SS-OCT in both eyes showed marked thinning of the fovea, and marked disorganization and atrophy of the outer retinal layers. The choroid showed diffuse loss of the choriocapillaris and of the larger choroidal vessels with subsequent loss of the normal bowl-shaped configuration. SS-OCTA showed diffuse loss of the choriocap-

illaris with unveiling of the larger choroidal vessels (**Figure 4(A)** and **(B)**).

MFC and PIC are idiopathic chorioretinal inflammatory disorders that are grouped under the spectrum of white dot syndromes. The phenotypic features of both entities overlap in many aspects to the extent that they are considered variants of the same disorder rather than separate clinical entities. MFC/PIC have predilection for young myopic females. Their hallmark is the development of yellowishwhite chorioretinal lesions scattered throughout the posterior pole and the

periphery of the fundus. Histologically, these lesions are composed of aggregates of inflammatory cells beneath the RPE that cause multifocal conical elevation of the RPE cell layer in the form of inflammatory PED. These lesions eventually breakthrough the RPE into the sub-retinal space with subsequent release of the inflammatory infiltrates into the outer retina. Recurrent episodes of inflammation cause structural retinal damage. The chorioretinal pathologic cascade is associated with vitritis that is most marked in cases of MFC compared to PIC. As chronicity ensues these lesions develop into punched-out chorioretinal scars [38–41]. Secondary CNV formation is a frequent complication, mounting up to one-third of cases, though some studies report much higher incidence [42–44]. CNV superimposed

*2.1.3 Multifocal choroiditis (MFC) and punctate inner choroidopathy (PIC)*

**92**

*(A) Top: Color fundus photo and FFA of both eyes of a 28-year-old female in chronic stage of serpiginous choroiditis. Note the diffuse plaques of sub-retinal fibrosis in the peripapillary area that radiate to involve almost the entire posterior pole. RPE pigment clumps formation is seen in the center of these lesions. On the corresponding FFA, these lesions show central blocked fluorescence surrounded by hyperfluorescent rim due to staining of the scar tissue. Bottom: Corresponding horizontal line scan (9.0 mm) SS-OCT (right), and vertical line scan (9.0 mm) SS-OCT (left) of both eyes show marked thinning of the choroid (255 and 240 μm in the right and left eyes, respectively). Note the irregular choroidal contour, marked thinning of the choriocapillaris and enhanced visualization of larger-sized choroidal vessels. The RPE-Bruch's complex, ellipsoid zone, ELM and IS/OS could not be identified and are replaced by amorphous hyper-reflective deposits. Note the foveal thinning and marked disorganization of the overlying neurosensory retina. (B) En-face SS-OCTA image and the corresponding flow density map of the choriocapillaris of the right eye in a 3 × 3 mm field. There are scattered sharply circumscribed areas of flow-voids due to loss of choriocapillaris (white arrows) with corresponding blue shades on the flow-density map. Note the enhanced visualization of the hyperintense signal of the larger choroidal vessels at the edges of the flow-voids and elsewhere due to combination of compensatory dilatation and anterior displacement of Sattler's vessel layer.*

on MFC/PIC is usually a type 2 variant and is notoriously difficult to detect or to follow-up its response to anti-vascular endothelial growth factor (anti-VEGF) agents using conventional angiography techniques or structural OCT because it is characterized by minimal leakage on angiography and fluid accumulation on OCT, in addition to overlapping angiographic and OCT features with MFC/PIC-induced inflammation [10].

#### *A Practical Guide to Clinical Application of OCT in Ophthalmology*

#### **Figure 4.**

*(A) Color fundus photo of both eyes of a 37-year-old female with advanced posterior pole scarring secondary to serpiginous choroiditis. The retinal layers and underlying choroidal vasculature have virtually disappeared from the peripapillary and macular areas with secondary RPE pigment clumps formation. (B) Left: Radial scan SS-OCT of the right eye. Note almost complete disappearance of the choriocapillaris. The larger-sized choroidal vessel layer is thinned and has even disappeared in some areas (white arrows). In the sub-foveal area, a combination of severe atrophy of the neurosensory retina, RPE, choriocapillaris and marked anterior bowing of the choroid has resulted in enhanced optical reflectance of the choroid and underlying sclera. Right: Corresponding en-face SS-OCTA image at the level of the choriocapillaris in a 3 × 3 mm field. The choriocapillaris has been reduced to a small patch (white arrowheads) with near complete wiping of remaining parts and readily visualization of the larger choroidal vessels.*

#### *2.1.3.1 Case presentation*

A 37-year-old female who is a known case of PIC presenting with drop of vision in the right eye of approximately 2-month duration. BCVA was 5/60 and 6/12 in the right and left eyes, respectively. Fundus examination of the right eye revealed features of myopic fundus with multiple discrete punched-out sub-retinal lesions scattered in the posterior pole and extending towards the equator. Most lesions were yellowish-white in color, whereas few lesions demonstrated variable degree of pigmentation. The foveal area showed a grayish sub-retinal lesion that exhibited typical features of classic CNV on FFA. SS-OCT confirmed the presence of type 2 CNV. SS-OCTA demonstrated hyperintense signal and characteristic features of active CNV. The patient received anti-VEGF injections for the right eye. At this stage her left fundus showed tessellated appearance with peripapillary atrophy due to myopia without signs of PIC. The patient returned 9 months later with complaints of metamorphopsia in the left eye. BCVA in the left eye has dropped to 6/18. Fundus examination revealed multiple scattered subretinal creamy white lesions. She was started on oral steroid therapy and the fundus

**95**

*Swept-Source Optical Coherence Tomography and Optical Coherence Tomography Angiography…*

lesions went onto remission though metamorphopsia persisted. Three months later she presented with worsening metamorphopsia and more drop of vision. BCVA in the left

SS-OCT showed sub-RPE hyperreflective knob-like projections either confined to the sub-RPE space or breaking through into the sub-retinal space. SS-OCTA of the choriocapillaris revealed scattered foci of flow-voids corresponding to the location of the scarred punched-out lesions in the fundus. The foveal lesion suggestive of neovascular growth on color fundus photo and on FFA appeared as a sub-foveal amorphous hyperreflective material above the RPE suggestive of type 2 CNV with minimal overlying fluid. SS-OCTA at the level of the outer retina confirmed the hyperintense signal of

flow within neovascular complex (**Figures 5(A)** and **(B)** and **6(A)** and **(B)**).

eye after 3 ranibizumab injections and 7-month follow-up was 6/24.

A 31-year-old female who is a known case of MFC presented with drop of vision in the left eye of approximately 3 weeks duration. BCVA was 6/6 and 3/60 in the right and left eyes, respectively. Fundus examination in the left eye revealed a sub-foveal yellowish-white lesion approximately 1 disc diameter (DD) in size. The posterior pole temporal to the lesion showed sub-retinal punched-out healed lesions of old episode of MFC. FFA demonstrated sub-foveal classic CNV formation. SS-OCT and SS-OCTA confirmed presence of neovascular growth. The patient started anti-VEGF injection that caused regression of the CNV. Her BCVA in the left

SS-OCT examination revealed the presence of type 2 CNV with minimal overlying sub-retinal fluid. SS-OCTA revealed the hyperintense signal characteristic of flow within active neovascular network. On follow-up visits after the patient received anti-VEGF intravitreal injections, the morphology of the CNV on SS-OCT showed minimal variation. On the other hand, SS-OCTA was conclusive in documenting the regression of the CNV in response to anti-VEGF treatment (**Figure 7(A)** and **(B)**).

Ocular toxoplasmosis is an infectious granulomatous posterior uveitis. The disease is considered the most common cause of posterior uveitis worldwide. Ocular involvement occurs as part of systemic toxoplasmosis. Infection is either congenital via transplacental transmission of the protozoan *Toxoplasma gondii* form an infected mother, or acquired due to ingestion of contaminated water or food or ingestion of undercooked meat containing toxoplasma cysts [45]. The clinical presentation is in the form of focal necrotizing retinochoroiditis in the posterior pole. The lesion can develop de novo or can present in the form of a satellite lesion, which is an acute focus of retinochoroiditis developing adjacent to an old chorioretinal scar. The lesion is associated with overlying vitritis which could be severe enough to obscure visualization of the fundus apart from the yellowishwhite necrotizing focal lesion giving rise to the characteristic spotlight-in-the-fog appearance. As the acute episode subsides, a quiescent hyperpigmented scar

eye was 6/36. FFA, SS-OCT, and SS-OCTA revealed presence of CNV.

*DOI: http://dx.doi.org/10.5772/intechopen.84245*

*2.1.3.1.1 SS-OCT and SS-OCTA features*

*2.1.3.2.1 SS-OCT and SS-OCTA features*

*2.1.3.2 Case presentation*

**2.2 Infectious uveitides**

*2.2.1 Toxoplasmosis*

*Swept-Source Optical Coherence Tomography and Optical Coherence Tomography Angiography… DOI: http://dx.doi.org/10.5772/intechopen.84245*

lesions went onto remission though metamorphopsia persisted. Three months later she presented with worsening metamorphopsia and more drop of vision. BCVA in the left eye was 6/36. FFA, SS-OCT, and SS-OCTA revealed presence of CNV.

### *2.1.3.1.1 SS-OCT and SS-OCTA features*

SS-OCT showed sub-RPE hyperreflective knob-like projections either confined to the sub-RPE space or breaking through into the sub-retinal space. SS-OCTA of the choriocapillaris revealed scattered foci of flow-voids corresponding to the location of the scarred punched-out lesions in the fundus. The foveal lesion suggestive of neovascular growth on color fundus photo and on FFA appeared as a sub-foveal amorphous hyperreflective material above the RPE suggestive of type 2 CNV with minimal overlying fluid. SS-OCTA at the level of the outer retina confirmed the hyperintense signal of flow within neovascular complex (**Figures 5(A)** and **(B)** and **6(A)** and **(B)**).

#### *2.1.3.2 Case presentation*

*A Practical Guide to Clinical Application of OCT in Ophthalmology*

**94**

*2.1.3.1 Case presentation*

*parts and readily visualization of the larger choroidal vessels.*

**Figure 4.**

A 37-year-old female who is a known case of PIC presenting with drop of vision in the right eye of approximately 2-month duration. BCVA was 5/60 and 6/12 in the right and left eyes, respectively. Fundus examination of the right eye revealed features of myopic fundus with multiple discrete punched-out sub-retinal lesions scattered in the posterior pole and extending towards the equator. Most lesions were yellowish-white in color, whereas few lesions demonstrated variable degree of pigmentation. The foveal area showed a grayish sub-retinal lesion that exhibited typical features of classic CNV on FFA. SS-OCT confirmed the presence of type 2 CNV. SS-OCTA demonstrated hyperintense signal and characteristic features of active CNV. The patient received anti-VEGF injections for the right eye. At this stage her left fundus showed tessellated appearance with peripapillary atrophy due to myopia without signs of PIC. The patient returned 9 months later with complaints of metamorphopsia in the left eye. BCVA in the left eye has dropped to 6/18. Fundus examination revealed multiple scattered subretinal creamy white lesions. She was started on oral steroid therapy and the fundus

*(A) Color fundus photo of both eyes of a 37-year-old female with advanced posterior pole scarring secondary to serpiginous choroiditis. The retinal layers and underlying choroidal vasculature have virtually disappeared from the peripapillary and macular areas with secondary RPE pigment clumps formation. (B) Left: Radial scan SS-OCT of the right eye. Note almost complete disappearance of the choriocapillaris. The larger-sized choroidal vessel layer is thinned and has even disappeared in some areas (white arrows). In the sub-foveal area, a combination of severe atrophy of the neurosensory retina, RPE, choriocapillaris and marked anterior bowing of the choroid has resulted in enhanced optical reflectance of the choroid and underlying sclera. Right: Corresponding en-face SS-OCTA image at the level of the choriocapillaris in a 3 × 3 mm field. The choriocapillaris has been reduced to a small patch (white arrowheads) with near complete wiping of remaining* 

A 31-year-old female who is a known case of MFC presented with drop of vision in the left eye of approximately 3 weeks duration. BCVA was 6/6 and 3/60 in the right and left eyes, respectively. Fundus examination in the left eye revealed a sub-foveal yellowish-white lesion approximately 1 disc diameter (DD) in size. The posterior pole temporal to the lesion showed sub-retinal punched-out healed lesions of old episode of MFC. FFA demonstrated sub-foveal classic CNV formation. SS-OCT and SS-OCTA confirmed presence of neovascular growth. The patient started anti-VEGF injection that caused regression of the CNV. Her BCVA in the left eye after 3 ranibizumab injections and 7-month follow-up was 6/24.

#### *2.1.3.2.1 SS-OCT and SS-OCTA features*

SS-OCT examination revealed the presence of type 2 CNV with minimal overlying sub-retinal fluid. SS-OCTA revealed the hyperintense signal characteristic of flow within active neovascular network. On follow-up visits after the patient received anti-VEGF intravitreal injections, the morphology of the CNV on SS-OCT showed minimal variation. On the other hand, SS-OCTA was conclusive in documenting the regression of the CNV in response to anti-VEGF treatment (**Figure 7(A)** and **(B)**).

#### **2.2 Infectious uveitides**

#### *2.2.1 Toxoplasmosis*

Ocular toxoplasmosis is an infectious granulomatous posterior uveitis. The disease is considered the most common cause of posterior uveitis worldwide. Ocular involvement occurs as part of systemic toxoplasmosis. Infection is either congenital via transplacental transmission of the protozoan *Toxoplasma gondii* form an infected mother, or acquired due to ingestion of contaminated water or food or ingestion of undercooked meat containing toxoplasma cysts [45]. The clinical presentation is in the form of focal necrotizing retinochoroiditis in the posterior pole. The lesion can develop de novo or can present in the form of a satellite lesion, which is an acute focus of retinochoroiditis developing adjacent to an old chorioretinal scar. The lesion is associated with overlying vitritis which could be severe enough to obscure visualization of the fundus apart from the yellowishwhite necrotizing focal lesion giving rise to the characteristic spotlight-in-the-fog appearance. As the acute episode subsides, a quiescent hyperpigmented scar

#### **Figure 5.**

*(A) Top: Color fundus photo and FFA of the right and left eyes, respectively of a 37-year-old female with PIC. The right fundus shows multiple yellowish-white punched-out lesions scattered in the posterior pole and extending towards the equator. Some of these lesions show RPE clump deposition. The foveal area shows a sub-retinal grayish lesion. The fundus shows features of myopia as tessellated appearance and peripapillary chorioretinal atrophy. On FFA, the punched-out lesions seen in the color photo demonstrate early hyperfluorescence that gradually increases in intensity throughout successive frames due to combined pooling in PED and late staining. The lesions maintain sharply circumscribed boundaries without leakage of dye. Some of these lesions show blocked fluorescence due to masking by RPE clump formation. The foveal lesion shows early hyperfluorescence that gradually increases in intensity throughout successive frames due to minimal leakage. Color fundus photo and FFA of the left eye show myopic features. Bottom left: Radial scan SS-OCT of the right eye shows knob-like projections in the sub-RPE space (white arrow). One of these projections broke through into the sub-retinal space (asterisk). Note the sub-foveal hyperreflective amorphous lesion with minimal overlying fluid, suggestive of type 2 CNV formation (open arrow). Bottom right: Radial scan SS-OCT of the left eye showed choroidal thinning due to myopia. (B) Top: En-face SS-OCTA image in a 3 × 3 mm field at the level of the outer retina and the corresponding flow-density map show the hyperintense signal characteristic of flow within neovascular tissue (white arrow). The CNV shows dense arborization and anastomosis, which are characteristic of activity. Bottom: En-face SS-OCTA image in a 6 × 6 mm field at the level of the choriocapillaris and the corresponding flow-density map show the moth-eaten appearance of the choriocapillaris due to presence of scattered flow-voids (white open arrows) denoting foci of vascular atrophy and that correspond to the location of the punched-out lesions seen in the color photo.*

**97**

**Figure 6.**

*Swept-Source Optical Coherence Tomography and Optical Coherence Tomography Angiography…*

*(A) Top left: Color photo of the left fundus of the same patient in Figure 5. Note the multiple creamy white lesions that developed in the posterior pole. Note the indistinct boundaries of some of these lesions (white open arrows), which indicate their recent onset compared to the atrophic punched-out appearance of the older lesions seen in the right fundus. Right: En-face SS-OCTA images in a 3 × 3 mm field of the outer retina and of the choriocapillaris. Note the normal appearance of the avascular outer retina, and the development of multiple foci of flow-voids in the choriocapillaris corresponding to the sub-retinal lesions seen in color photo. Bottom left: High-definition line scan (12.0 mm) SS-OCT of the left eye. Note the newly developed hyperreflective knob-like projections of the RPE cell layer (white open arrows). Some of these projections broke through the RPE into the sub-retinal space (white closed arrows). (B) Top left: Color fundus photo and FFA of the same patient 3 months later. Note the newly developed neovascular membrane in the foveal area. Top right: Radial scan SS-OCT shows newly developed sub-foveal hyperreflective amorphous lesion above the RPE, suggestive of type 2 CNV formation. Bottom left: En-face SS-OCTA image and the corresponding flow-density map of the outer retina in a 3 × 3 mm field show the hyperintense signal of the newly developed CNV. The dense arborization and anastomosis within the lesion are indicators of activity. Bottom right: En-face SS-OCTA image and the corresponding flow-density map of the choriocapillaris in a 3 × 3 mm field show multifocal hypointense signal due to flow-voids, which could result from* 

*multifocal atrophy of choriocapillaris due to older lesions or from signal masking in newer ones.*

*DOI: http://dx.doi.org/10.5772/intechopen.84245*

*Swept-Source Optical Coherence Tomography and Optical Coherence Tomography Angiography… DOI: http://dx.doi.org/10.5772/intechopen.84245*

#### **Figure 6.**

*A Practical Guide to Clinical Application of OCT in Ophthalmology*

*(A) Top: Color fundus photo and FFA of the right and left eyes, respectively of a 37-year-old female with PIC. The right fundus shows multiple yellowish-white punched-out lesions scattered in the posterior pole and extending towards the equator. Some of these lesions show RPE clump deposition. The foveal area shows a sub-retinal grayish lesion. The fundus shows features of myopia as tessellated appearance and peripapillary chorioretinal atrophy. On FFA, the punched-out lesions seen in the color photo demonstrate early hyperfluorescence that gradually increases in intensity throughout successive frames due to combined pooling in PED and late staining. The lesions maintain sharply circumscribed boundaries without leakage of dye. Some of these lesions show blocked fluorescence due to masking by RPE clump formation. The foveal lesion shows early hyperfluorescence that gradually increases in intensity throughout successive frames due to minimal leakage. Color fundus photo and FFA of the left eye show myopic features. Bottom left: Radial scan SS-OCT of the right eye shows knob-like projections in the sub-RPE space (white arrow). One of these projections broke through into the sub-retinal space (asterisk). Note the sub-foveal hyperreflective amorphous lesion with minimal overlying fluid, suggestive of type 2 CNV formation (open arrow). Bottom right: Radial scan SS-OCT of the left eye showed choroidal thinning due to myopia. (B) Top: En-face SS-OCTA image in a 3 × 3 mm field at the level of the outer retina and the corresponding flow-density map show the hyperintense signal characteristic of flow within neovascular tissue (white arrow). The CNV shows dense arborization and anastomosis, which are characteristic of activity. Bottom: En-face SS-OCTA image in a 6 × 6 mm field at the level of the choriocapillaris and the corresponding flow-density map show the moth-eaten appearance of the choriocapillaris due to presence of scattered flow-voids (white open arrows) denoting foci of vascular atrophy* 

*and that correspond to the location of the punched-out lesions seen in the color photo.*

**96**

**Figure 5.**

*(A) Top left: Color photo of the left fundus of the same patient in Figure 5. Note the multiple creamy white lesions that developed in the posterior pole. Note the indistinct boundaries of some of these lesions (white open arrows), which indicate their recent onset compared to the atrophic punched-out appearance of the older lesions seen in the right fundus. Right: En-face SS-OCTA images in a 3 × 3 mm field of the outer retina and of the choriocapillaris. Note the normal appearance of the avascular outer retina, and the development of multiple foci of flow-voids in the choriocapillaris corresponding to the sub-retinal lesions seen in color photo. Bottom left: High-definition line scan (12.0 mm) SS-OCT of the left eye. Note the newly developed hyperreflective knob-like projections of the RPE cell layer (white open arrows). Some of these projections broke through the RPE into the sub-retinal space (white closed arrows). (B) Top left: Color fundus photo and FFA of the same patient 3 months later. Note the newly developed neovascular membrane in the foveal area. Top right: Radial scan SS-OCT shows newly developed sub-foveal hyperreflective amorphous lesion above the RPE, suggestive of type 2 CNV formation. Bottom left: En-face SS-OCTA image and the corresponding flow-density map of the outer retina in a 3 × 3 mm field show the hyperintense signal of the newly developed CNV. The dense arborization and anastomosis within the lesion are indicators of activity. Bottom right: En-face SS-OCTA image and the corresponding flow-density map of the choriocapillaris in a 3 × 3 mm field show multifocal hypointense signal due to flow-voids, which could result from multifocal atrophy of choriocapillaris due to older lesions or from signal masking in newer ones.*

#### *A Practical Guide to Clinical Application of OCT in Ophthalmology*

#### **Figure 7.**

*(A) Left: Color fundus photo and FFA of the left eye of a 31-year-old female with MFC complicated by CNV formation. The fundus shows a sub-foveal yellowish-white lesion approximately 1 DD in size. Note the multiple whitish punched-out lesions located temporally and denote healed MFC lesions (white arrows). On FFA, the sub-foveal lesion demonstrates early well-circumscribed hyperfluorescence with late profuse leakage beyond the boundaries of the lesion. Right: Radial scan mode SS-OCT shows hyperreflective amorphous lesion lying entirely above the RPE with minimal overlying hyporeflective sub-retinal fluid. Note the minimal variation in the lesion morphology in follow-up SS-OCT scans, hence inconclusive information on CNV response to therapy. (B) En-face SS-OCTA images of the outer retina and the corresponding flow-density maps in a 3 × 3 mm field at initial presentation (left) and during follow-up visits after receiving anti-VEGF treatment (middle and right). At presentation, SS-OCTA of the outer retina demonstrated the hyperintense signal characteristic of flow within neovascular growth. Note the extensive anastomosis and dense arborization within the lesion, which indicate active CNV. Note the unequivocal SS-OCTA depiction of gradual regression of the CNV lesion during follow-up visits in response to anti-VEGF treatment.*

**99**

*Swept-Source Optical Coherence Tomography and Optical Coherence Tomography Angiography…*

develops which could lead to profound visual loss should it affect the macula or the papillomacular bundle [18]. On structural OCT, an active lesion features thickening and disorganization of the neurosensory retina, thickening of the choroid underlying the lesion and evidence of vitritis in the form of hyperreflective dots and thickening of the posterior hyaloid. As the lesion heals, the patient develops a chorioretinal scar with marked thinning and disorganization of the neurosensory retina and thinning of the underlying choroid. Choroidal neovascularization and vitreoretinal interface disturbances in the form of vitreoretinal tractional bands

A 13-year-old male presented to our clinic complaining of diminution of vision in his right eye of 3 days duration. Fundus examination revealed whitish retinal lesion inferonasal to the ONH with feathery indistinct edges and overlying vitritis. The ONH was inflamed with blurred edges. BCVA was 6/60 and 6/6 in the right and left eyes, respectively. Due to proximity of the lesion to the optic disc and the macula, we started the patient on oral sulfadiazine, pyrimethamine, and folinic acid. Oral steroids were started 24 h after initiation of antimicrobial regimen. At 2-month follow-up visit, the fundus showed resolution of previously noted vitritis and disc hyperemia, and regression of the retinal lesion, which appeared smaller in size and had well-defined boundaries. BCVA in the right eye

SS-OCT showed focal thickening with complete disorganization of the neurosensory retina, disruption of the ELM, IS/OS, and RPE cell layer with focal thickening of the choroid beneath the lesion. The overlying vitreous showed dense infiltration with hyperreflective foci indicating vitritis. SS-OCTA showed localized hypointense signal in the SCP, DCP, and choriocapillaris corresponding due to the masking by the focal lesion. During follow-up the previously noted hypointense signal regressed in size in proportion to regression of the lesion

A 26-year-old female presented to our clinic complaining of diminution of vision in her right eye of 1 week duration. Fundus examination revealed yellowishwhite retinal lesion composed of a larger focus and smaller adjacent lesions overlying the papillomacular bundle. BCVA was 6/24 and 6/6 in the right and left eyes, respectively. As the lesion involved the papillomacular bundle, the patient was started on oral sulfadiazine, pyrimethamine, and folinic acid. Oral steroids were

SS-OCT showed focal thickening with complete disorganization of the neurosensory retina, disruption of the ELM, IS/OS, and RPE cell layer with focal thickening of the choroid beneath the lesion. The posterior hyaloid was thickened and partially attached to the focal retinal lesion. SS-OCTA showed localized hypointense signal in the SCP, DCP, and choriocapillaris corresponding to the masking effect of the focal

started 24 h after initiation of antimicrobial regimen.

*2.2.1.2.1 SS-OCT and SS-OCTA features*

lesion (**Figure 9(A)** and **(B)**).

*DOI: http://dx.doi.org/10.5772/intechopen.84245*

can develop [17, 18, 46].

*2.2.1.1 Case presentation*

improved to 6/6.

(**Figure 8(A)** and **(B)**).

*2.2.1.2 Case presentation*

*2.2.1.1.1 SS-OCT and SS-OCTA features*

*Swept-Source Optical Coherence Tomography and Optical Coherence Tomography Angiography… DOI: http://dx.doi.org/10.5772/intechopen.84245*

develops which could lead to profound visual loss should it affect the macula or the papillomacular bundle [18]. On structural OCT, an active lesion features thickening and disorganization of the neurosensory retina, thickening of the choroid underlying the lesion and evidence of vitritis in the form of hyperreflective dots and thickening of the posterior hyaloid. As the lesion heals, the patient develops a chorioretinal scar with marked thinning and disorganization of the neurosensory retina and thinning of the underlying choroid. Choroidal neovascularization and vitreoretinal interface disturbances in the form of vitreoretinal tractional bands can develop [17, 18, 46].

### *2.2.1.1 Case presentation*

*A Practical Guide to Clinical Application of OCT in Ophthalmology*

**98**

*during follow-up visits in response to anti-VEGF treatment.*

**Figure 7.**

*(A) Left: Color fundus photo and FFA of the left eye of a 31-year-old female with MFC complicated by CNV formation. The fundus shows a sub-foveal yellowish-white lesion approximately 1 DD in size. Note the multiple whitish punched-out lesions located temporally and denote healed MFC lesions (white arrows). On FFA, the sub-foveal lesion demonstrates early well-circumscribed hyperfluorescence with late profuse leakage beyond the boundaries of the lesion. Right: Radial scan mode SS-OCT shows hyperreflective amorphous lesion lying entirely above the RPE with minimal overlying hyporeflective sub-retinal fluid. Note the minimal variation in the lesion morphology in follow-up SS-OCT scans, hence inconclusive information on CNV response to therapy. (B) En-face SS-OCTA images of the outer retina and the corresponding flow-density maps in a 3 × 3 mm field at initial presentation (left) and during follow-up visits after receiving anti-VEGF treatment (middle and right). At presentation, SS-OCTA of the outer retina demonstrated the hyperintense signal characteristic of flow within neovascular growth. Note the extensive anastomosis and dense arborization within the lesion, which indicate active CNV. Note the unequivocal SS-OCTA depiction of gradual regression of the CNV lesion* 

A 13-year-old male presented to our clinic complaining of diminution of vision in his right eye of 3 days duration. Fundus examination revealed whitish retinal lesion inferonasal to the ONH with feathery indistinct edges and overlying vitritis. The ONH was inflamed with blurred edges. BCVA was 6/60 and 6/6 in the right and left eyes, respectively. Due to proximity of the lesion to the optic disc and the macula, we started the patient on oral sulfadiazine, pyrimethamine, and folinic acid. Oral steroids were started 24 h after initiation of antimicrobial regimen. At 2-month follow-up visit, the fundus showed resolution of previously noted vitritis and disc hyperemia, and regression of the retinal lesion, which appeared smaller in size and had well-defined boundaries. BCVA in the right eye improved to 6/6.

#### *2.2.1.1.1 SS-OCT and SS-OCTA features*

SS-OCT showed focal thickening with complete disorganization of the neurosensory retina, disruption of the ELM, IS/OS, and RPE cell layer with focal thickening of the choroid beneath the lesion. The overlying vitreous showed dense infiltration with hyperreflective foci indicating vitritis. SS-OCTA showed localized hypointense signal in the SCP, DCP, and choriocapillaris corresponding due to the masking by the focal lesion. During follow-up the previously noted hypointense signal regressed in size in proportion to regression of the lesion (**Figure 8(A)** and **(B)**).

#### *2.2.1.2 Case presentation*

A 26-year-old female presented to our clinic complaining of diminution of vision in her right eye of 1 week duration. Fundus examination revealed yellowishwhite retinal lesion composed of a larger focus and smaller adjacent lesions overlying the papillomacular bundle. BCVA was 6/24 and 6/6 in the right and left eyes, respectively. As the lesion involved the papillomacular bundle, the patient was started on oral sulfadiazine, pyrimethamine, and folinic acid. Oral steroids were started 24 h after initiation of antimicrobial regimen.

#### *2.2.1.2.1 SS-OCT and SS-OCTA features*

SS-OCT showed focal thickening with complete disorganization of the neurosensory retina, disruption of the ELM, IS/OS, and RPE cell layer with focal thickening of the choroid beneath the lesion. The posterior hyaloid was thickened and partially attached to the focal retinal lesion. SS-OCTA showed localized hypointense signal in the SCP, DCP, and choriocapillaris corresponding to the masking effect of the focal lesion (**Figure 9(A)** and **(B)**).

#### **Figure 8.**

*(A) Top left: Color fundus photo and FFA of the right eye of a 13-year-old male with acute toxoplasma retinochoroiditis. The fundus shows a whitish retinal lesion inferotemporal to ONH with blurred edges and overlying vitritis. The ONH shows hyperemia and engorged peripapillary vessels. The corresponding FFA shows early localized blocked fluorescence corresponding in size and location to the lesion seen in color photo. Later frames show leakage from adjacent focal retinal vasculitis. The ONH demonstrates hyperfluorescence due to leakage form inflamed optic disc vessels. Bottom left: High-definition line scan (12.0 mm) SS-OCT shows focal thickening of the retina temporal to the optic disc with marked disorganization of the retinal layers and increased optical reflectance giving the affected part a blurred hyperreflective appearance known as the smudge effect, and causing optical shadowing of the underlying choroid. Note focal disruption of the ELM, and IS/ OS layers in the area of the lesion. The choroid underlying the focal lesion is markedly thickened (628 μm), with focal blurring of the CSI. The overlying vitreous shows dense infiltration with hyperreflective foci, which represent inflammatory cell infiltrates. Right: En-face SS-OCTA images in a 6 × 6 mm field of the SCP, DCP, outer retina, and the choriocapillaris show hypointense signal caused by masking of the vascular layers and overshadowing of the outer retina by the focal lesion and overlying vitreous opacity. (B) Top left: Color fundus photo and FFA of the right eye at 2-month follow-up visit. Note resolution of vitritis, optic disc swelling, and a smaller size well-defined residual lesion. The corresponding FFA shows significantly improved retinal vasculitis and minimal leakage from the optic disc vessels. Bottom left: Radial scan SS-OCT shows complete resolution of choroidal inflammation (choroidal thickening measured 238 μm), with a well-defined CSI. The previously noted hyperreflective foci in the vitreous disappeared almost completely. There is significant improvement in the previously noted focal thickening of the retina, though with persistent disorganization of retinal layers and disruption of ELM and IS/OS layers. Top right: En-face SS-OCTA images of the SCP, DCP, outer retina, and the choriocapillaris in a 6 × 6 mm field show marked regression of the previously noted hypointense signal and improved visualization of the normal architecture of the vascular layers and of the outer retina.*

**101**

*outer retina by the focal lesion.*

**Figure 9.**

*Swept-Source Optical Coherence Tomography and Optical Coherence Tomography Angiography…*

*(A) Top left: Color fundus photo and FFA of the right eye of a 26-year-old female with acute toxoplasma retinochoroiditis. Note the yellowish-white lesions overlying the papillomacular bundle that consist of a single larger focus and adjacent smaller ones. The partially blurred appearance of the optic disc and the papillomacular bundle is caused by vitritis overlying the lesion. FFA shows localized blocked fluorescence corresponding in size and location to the lesion seen in color photo. In later frames, leakage from adjacent focal retinal vasculitis causes hyperfluorescence. The ONH demonstrates hyperfluorescence due to leakage from inflamed disc vessels. Top middle: Radial scan SS-OCT shows focal thickening of the retina temporal to the optic disc with marked disorganization of the retinal layers and increased optical reflectance giving the affected part a blurred hyperreflective appearance known as the smudge effect, and causing optical shadowing of the underlying choroid. The ELM, IS/OS layers are disrupted. The choroid underlying the focal lesion is thickened. The overlying vitreous shows thickening of the posterior hyaloid which is partially attached to the focal retinal lesion. Top right: A zoom-in on the attachment between the focal retinal lesion and the posterior hyaloid. Bottom: High-definition line scan (12.0 mm) SS-OCT with the enhanced vitreous visualization (EVV) mode turned-on. Note the multiple hyperreflective dots scattered in the vitreous cavity and deposited along the posterior hyaloid face. (B) En-face SS-OCTA images of the SCP, DCP, outer retina and the choriocapillaris in a 6 × 6 mm field show hypointense signal caused by masking of the vascular layers and overshadowing of the* 

*DOI: http://dx.doi.org/10.5772/intechopen.84245*

*Swept-Source Optical Coherence Tomography and Optical Coherence Tomography Angiography… DOI: http://dx.doi.org/10.5772/intechopen.84245*

#### **Figure 9.**

*A Practical Guide to Clinical Application of OCT in Ophthalmology*

*(A) Top left: Color fundus photo and FFA of the right eye of a 13-year-old male with acute toxoplasma retinochoroiditis. The fundus shows a whitish retinal lesion inferotemporal to ONH with blurred edges and overlying vitritis. The ONH shows hyperemia and engorged peripapillary vessels. The corresponding FFA shows early localized blocked fluorescence corresponding in size and location to the lesion seen in color photo. Later frames show leakage from adjacent focal retinal vasculitis. The ONH demonstrates hyperfluorescence due to leakage form inflamed optic disc vessels. Bottom left: High-definition line scan (12.0 mm) SS-OCT shows focal thickening of the retina temporal to the optic disc with marked disorganization of the retinal layers and increased optical reflectance giving the affected part a blurred hyperreflective appearance known as the smudge effect, and causing optical shadowing of the underlying choroid. Note focal disruption of the ELM, and IS/ OS layers in the area of the lesion. The choroid underlying the focal lesion is markedly thickened (628 μm), with focal blurring of the CSI. The overlying vitreous shows dense infiltration with hyperreflective foci, which represent inflammatory cell infiltrates. Right: En-face SS-OCTA images in a 6 × 6 mm field of the SCP, DCP, outer retina, and the choriocapillaris show hypointense signal caused by masking of the vascular layers and overshadowing of the outer retina by the focal lesion and overlying vitreous opacity. (B) Top left: Color fundus photo and FFA of the right eye at 2-month follow-up visit. Note resolution of vitritis, optic disc swelling, and a smaller size well-defined residual lesion. The corresponding FFA shows significantly improved retinal vasculitis and minimal leakage from the optic disc vessels. Bottom left: Radial scan SS-OCT shows complete resolution of choroidal inflammation (choroidal thickening measured 238 μm), with a well-defined CSI. The previously noted hyperreflective foci in the vitreous disappeared almost completely. There is significant improvement in the previously noted focal thickening of the retina, though with persistent disorganization of retinal layers and disruption of ELM and IS/OS layers. Top right: En-face SS-OCTA images of the SCP, DCP, outer retina, and the choriocapillaris in a 6 × 6 mm field show marked regression of the previously noted hypointense signal and* 

*improved visualization of the normal architecture of the vascular layers and of the outer retina.*

**100**

**Figure 8.**

*(A) Top left: Color fundus photo and FFA of the right eye of a 26-year-old female with acute toxoplasma retinochoroiditis. Note the yellowish-white lesions overlying the papillomacular bundle that consist of a single larger focus and adjacent smaller ones. The partially blurred appearance of the optic disc and the papillomacular bundle is caused by vitritis overlying the lesion. FFA shows localized blocked fluorescence corresponding in size and location to the lesion seen in color photo. In later frames, leakage from adjacent focal retinal vasculitis causes hyperfluorescence. The ONH demonstrates hyperfluorescence due to leakage from inflamed disc vessels. Top middle: Radial scan SS-OCT shows focal thickening of the retina temporal to the optic disc with marked disorganization of the retinal layers and increased optical reflectance giving the affected part a blurred hyperreflective appearance known as the smudge effect, and causing optical shadowing of the underlying choroid. The ELM, IS/OS layers are disrupted. The choroid underlying the focal lesion is thickened. The overlying vitreous shows thickening of the posterior hyaloid which is partially attached to the focal retinal lesion. Top right: A zoom-in on the attachment between the focal retinal lesion and the posterior hyaloid. Bottom: High-definition line scan (12.0 mm) SS-OCT with the enhanced vitreous visualization (EVV) mode turned-on. Note the multiple hyperreflective dots scattered in the vitreous cavity and deposited along the posterior hyaloid face. (B) En-face SS-OCTA images of the SCP, DCP, outer retina and the choriocapillaris in a 6 × 6 mm field show hypointense signal caused by masking of the vascular layers and overshadowing of the outer retina by the focal lesion.*
