**3.3 Solvent extraction**

Solvent extraction method or liquid-liquid method is done by separating compounds based on their part solubility [49]. The basic principle of the solvent extraction method is that between two immiscible solvents, the solute distributes itself in a fixed ratio, whereby one is usually water and the other is an organic solvent [50]. In this method, the plant material will be grinded in a mortar that contains anhydrous hexane Na2SO4, followed by four rounds of extraction with hexane to obtain the yellow extract, then this is followed by adding a sufficient amount of Norite A charcoal for all extracts to remove the yellow color after low-speed centrifugation, and eventually the solution will be concentrated under air stream at room temperature [37, 43, 49]. A newer method of solvent extraction, called the microwave-assisted simultaneous distillation-solvent extraction (MW-SDE), is faster and simpler and uses fewer solvents to determine volatile compounds than conventional methods [51].
