**3. Ethics**

This work was approved by the ethical committees of Showa Women's University and the NPO "International projects on food and health" and was conducted in accordance with The Code of Ethics of the World Medical Association (Declaration of Helsinki) for experiments.

## **4. Statistics**

The results are presented as means ± SD. Statistical significance of the differences between groups was calculated according by one-way ANOVA. When ANOVA indicated a significant difference (P < 0.05), the mean values of the treatment were compared using Tukey's least significant difference test at P < 0.05.
