*1.2.8 Treatment with glucoamylase of Rhizopus sp. (GAR)*

GAR enzyme shows a maximum activity at temperature of 40°C and a pH value of 4.5–6.3 [21]. Glucoamylase 1 exhibits maximum debranching activity and totally degrades starchy materials to fermentable sugars in SSF process. Saccharification using GAR was carried out in a temperature range of 55–60°C and a pH of 4.4–5.4 [23]; GAR was also stable in acidic pH while corn is used as substrate.
