**4. Conclusion**

The advantage of metagenomic method is that it allows us to study the genome of the bacterial community directly from the natural environment in which the function and role of certain bacteria on the environment can be determined. Studies have shown that metagenomics are a very useful technology in finding genes that can be applied in industrial, biotechnology, pharmaceutical and medical fields such as esterase, lipase, agarase, polymerase, polyketide synthases, chitinase and so on. Some potentially uncultured microbes and new genes were discovered through this study. In this study, metagenomic libraries using fosmid vectors were constructed from the bacterial community associated with A. tamiyavanichii. A total of 1501 fosmid clones ranging from 30 to 40 Kbp have been obtained and this is equivalent to 13 bacterial genomes. Finally, to our knowledge, the metagenomic library in this study was the first being constructed from the bacterial communities associated with toxic marine dinoflagellate. This collection of libraries can be used as a major source for finding new genes or pathways for biosynthesis and to study the interactions of dinoflagelates more profoundly, especially in the production of the saxitoxins as there is a hypothesis that the toxins produced by dinoflagelates are derived from bacteria.

## **Acknowledgements**

We thank all the people involved directly or indirectly in this study especially staffs from the School of Fisheries and Aquaculture Sciences, Universiti Malaysia Terengganu and Bachok Marine Research Station, University of Malaya.

**45**

**Author details**

and Gires Usup3

Muhd Danish-Daniel1,2\*, Mohd Ezhar Mohd Noor1

1 School of Fisheries and Aquaculture Sciences, Terengganu, Malaysia

3 University College Sabah Foundation, Kota Kinabalu, Sabah, Malaysia

© 2019 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/ by/3.0), which permits unrestricted use, distribution, and reproduction in any medium,

2 Institute of Marine Biotechnology, Terengganu, Malaysia

\*Address all correspondence to: mdda@umt.edu.my

provided the original work is properly cited.

, Yik Sung Yeong2

, Tan Min Pau1,2

*Construction and Analysis of Metagenome Library from Bacterial Community Associated…*

*DOI: http://dx.doi.org/10.5772/intechopen.88751*

### **Conflict of interest**

The authors declare no conflict of interest.

*Construction and Analysis of Metagenome Library from Bacterial Community Associated… DOI: http://dx.doi.org/10.5772/intechopen.88751*
