Meet the editors

Madan L. Nagpal, PhD, FLS, has been Research Associate Professor at the Department of Microbiology and Immunology, University of South Carolina, Columbia, SC, and Research Chemist at the Dorn Veterans Medical Center, Columbia, SC, USA for 10 plus years. He has published 40 research articles, one book chapter, and edited one book. He was awarded a Research and Productive Scholarship by the University of South Carolina

in 1990. He was funded by grants as a co-investigator by the National Institutes of Health and VA Merit Review. His contributions include gene regulation in steroid hormone biosynthesis and the characterization of *Bacillus* species. He is a member of the American Society of Cell Biology, American Association of Advancement of Science, American Association for Laboratory Animal Science, International Who's Who Professionals, and Marquis Who's Who in America.

Assist. Prof. Dr. Eng. Oana-Maria Boldura is licensed in genetic engineering with specialization in Molecular Biology (2005). She is a Master of Science in Genetic Manipulation (2007) and Pharmacy (2014), and has a PhD in Biotechnology (2010). From 2015 she has been an assistant professor at the Banat University of Agricultural Sciences and Veterinary Medicine, "King Michael I of Romania" Timisoara, Faculty of Veterinary Medicine,

Department of Chemistry, Biochemistry and Molecular Biology, and a researcher in the "Horia Cernescu" Research Unit. Her main scientific competences are in the fields of molecular biology, biotechnology, genomics, and biosecurity with technical aptitudes in the manipulation and experimentation of nucleic acid and proteins (purification, sequencing, gene expression studies, SDS-PAGE analysis of proteins fractions, "lab-on-chip" electrophoresis, and immunological testing). Her areas of interest are molecular forensic methods and apoptotic process pathways.

Dr. Cornel Baltă, MD, DVM, is licensed in veterinary medicine. He has a PhD in Veterinary Medicine (2011), qualified as a medical doctor (2018), and is a researcher at the Institute of Life Sciences, "Vasile Goldis" Western University Arad, Romania. His main scientific competences are in the fields of laboratory animals, molecular biology, physiology, histology, and clinical laboratory with competences and technical aptitudes in nucleic

acid examination by electrophoresis techniques, PCR and qPCR, and qualitative and quantitative determination of proteins by Western blot and microelectrophoresis. His areas of interest are the study of molecular mechanisms involved in the evolution of liver and gastrointestinal diseases and the study of molecular mechanisms involved in bone regeneration and remodeling using different types of polymers as scaffolds.

Dr. Shymaa Enany is an associate professor of microbiology and immunology at the Suez Canal University, Egypt. She received her PhD from the School of Medical and Dental Sciences, Niigata University, Japan, and completed her postdoctoral work in collaboration with many laboratories in San Diego, California, USA, and in Niigata, Japan. She is an editorial board member and reviewer in many journals and scientific associations and has many Contents

**Section 1**

*by Madan L. Nagpal*

*by Rajendra K. Bera*

**Section 2**

*Tan Min Pau and Gires Usup*

*by Xiangyuan Wan and Suowei Wu*

*and R. Holland Cheng*

*by Afiah Nasuha Aznan and Zakiah Jubri*

Platform Based on Hepatitis E Virus Nanoparticle

*by Shizuo G. Kamita, Mo A. Baikoghli, Luis M. de la Maza* 

**Preface III**

Genetic Engineering **1**

**Chapter 1 3**

**Chapter 2 11**

**Chapter 3 39**

Molecular Cloning **49**

**Chapter 4 51**

**Chapter 5 75**

**Chapter 6 103**

**Chapter 7 121**

*by Tapas Chakraborty, Sipra Mohapatra, Chimwar Wanglar and Dipak Pandey*

Molecular Cloning of Genic Male-Sterility Genes and Their Applications for Plant Heterosis via Biotechnology-based Male-sterility Systems

Silencing of Peroxiredoxin-4 in Anticancer Activity of Gamma-Tocotrienol

A Noninvasive, Orally Stable, Mucosa-Penetrating Polyvalent Vaccine

Community Associated with Toxic Dinoflagellate *Alexandrium tamiyavanichii*

Introductory Chapter: The Role of Genetic Engineering Technology in the Manipulation of Genetics of Organisms and Synthetic Biology

Synthetic Biology, Artificial Intelligence, and Quantum Computing

Construction and Analysis of Metagenome Library from Bacterial

*by Muhd Danish-Daniel, Mohd Ezhar Mohd Noor, Yik Sung Yeong,* 

Applied Molecular Cloning: Present and Future for Aquaculture

publications in eminent journals as well as books. She has excellent experience in bacterial genomics and proteomics.
