**Author details**

*Synthetic Biology - New Interdisciplinary Science*

of PSP toxins.

**4. Conclusion**

from bacteria.

**Acknowledgements**

**Conflict of interest**

Analysis of these partial sequences also revealed some genes that might be important in bacteria-algal interactions. One of the contigs was similar to the response regulator of the LuxR family protein from *Roseovarius* sp. This protein is known to be responsible for a variety of biological processes in the natural environment, including the quorum sensing and production of toxins [41]. In a complex community like during an algal bloom, this protein may play a significant role in determining the population structure and function through signaling or inducing the production of certain proteins [42]. Thus, it is believed that bacteria use this type of protein to adapt to the changing conditions around the phycosphere of dinoflagellate such as changes in nutrients, cell densities, and increasing concentration

The end-sequences obtained can not be used to describe the metabolic activity of each bacterial taxon involved but the analysis of the nucleotide sequences has shown that the constructed metagenomic library has great potential as a source to

The advantage of metagenomic method is that it allows us to study the genome of the bacterial community directly from the natural environment in which the function and role of certain bacteria on the environment can be determined. Studies have shown that metagenomics are a very useful technology in finding genes that can be applied in industrial, biotechnology, pharmaceutical and medical fields such as esterase, lipase, agarase, polymerase, polyketide synthases, chitinase and so on. Some potentially uncultured microbes and new genes were discovered through this study. In this study, metagenomic libraries using fosmid vectors were constructed from the bacterial community associated with A. tamiyavanichii. A total of 1501 fosmid clones ranging from 30 to 40 Kbp have been obtained and this is equivalent to 13 bacterial genomes. Finally, to our knowledge, the metagenomic library in this study was the first being constructed from the bacterial communities associated with toxic marine dinoflagellate. This collection of libraries can be used as a major source for finding new genes or pathways for biosynthesis and to study the interactions of dinoflagelates more profoundly, especially in the production of the saxitoxins as there is a hypothesis that the toxins produced by dinoflagelates are derived

We thank all the people involved directly or indirectly in this study especially staffs from the School of Fisheries and Aquaculture Sciences, Universiti Malaysia

Terengganu and Bachok Marine Research Station, University of Malaya.

The authors declare no conflict of interest.

study the physiology and function of the bacterial community involved.

**44**

Muhd Danish-Daniel1,2\*, Mohd Ezhar Mohd Noor1 , Yik Sung Yeong2 , Tan Min Pau1,2 and Gires Usup3

1 School of Fisheries and Aquaculture Sciences, Terengganu, Malaysia


\*Address all correspondence to: mdda@umt.edu.my

© 2019 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/ by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
