**2.1 Analyzed embryo cultivation medium: source of the material**

Embryo cultivation medium is usually being discarded during the IVF procedure although it can be used for multiple diagnostic and prognostic procedures. Certainly, ethical and moral guidelines and procedures must be observed, and the ethical board or other corresponding bodies must approve of the use of this material. The following experiments have been performed by using cultivation media from IVF media upon approval from the ethical boards of the Medical University in Vienna and the University of Linz in Austria. Samples were collected during different stages of embryo development and analyzed using methods established for analysis of low samples amounts.
