1. Introduction

The Environmental Protection Agency defined the green chemistry or sustainable chemistry as "the production of chemical products and processes which have the ability to reduce or eliminate the use or generation of hazardous substances." In recent years there is an expectation that chemists and chemical engineers should design greener and more sustainable chemical processes, and it was expected that this trend will continue to grow over the next few decades [1].

Studies on metal-based drugs offer promise in the fight against cancer, which is the main aim of the inorganic chemistry [2, 3].

The metal-based anticancer drugs are driven by the necessity to fill the gap in tumor chemotherapy in order to minimize the undesirable side effects and enlarge the spectrum of activity for more tumor types and metastatic cancers. The Schiff base structures play a key role in studying the mechanism of the transformation and racemization reaction in biological systems due to the similarity of their structures to the natural biological system [4–11].

Among various metal complexes, La(III) complexes have been intensively investigated due to their more physiological activities and lower toxicities after coordination with ligand, so, in recent years people have paid great interest to synthesis, DNA interaction, and anticancer activity of La(III) complexes [12–16]. metabolize MTT by viable cells. Two hundred microliter dimethyl sulfoxide

pounds to the cells) using the equation (A) test/(A) control 100.

2.4 Flow cytometry

DOI: http://dx.doi.org/10.5772/intechopen.83558

cytometric analysis.

2.6 Synthesis

powder form.

21

tip-to-collector distance (TCD) [39].

mercaptophenyl) benzamide H2L<sup>2</sup>

3. Results and discussion

3.1 Elemental analyses for nanometal complexes

(DMSO) was added to each well and shook again for 5 min at 150 rpm, and then the viability of the cells was calculated by measuring the optical density at 630 nm subtracted from optical density at 570 nm [21]. The percentage of viability cells was calculated by comparison with control cells (without adding the synthesized com-

Synthesis and Characterization of Nanocomplexes by Green Chemistry and Their Applications…

To discriminate living cells from dead cells or for cell cycle analysis, propidium iodide (PI) can be used according to Léonce et al. [22]. This analysis is based on the stoichiometric binding of PI to intracellular DNA. At the end, cells were washed with PBS and collected by trypsinization. Cells were then resuspended in warm PBS and fixed with 4 ml ice-cold ethanol. Finally, in darkness, cells were stained with 0.5 ml of warm PI solution; 7 ml of PI solution consists of 0.35 ml of PI stock solution (1 mg/ml), 0.7 ml RNase A stock solution (1 mg/ml), and 6 ml of PBS and incubated in darkness for 30 minutes. The samples were kept on ice until flow

2.5 Preparation of (chitosan-La(III) nanocomplex) composite nanofibers

Chitosan (CS) was dissolved in 50% acetic acids at room temperature under moderate stirring for 12 h to form a homogeneous solution, and then the solution of La complex nanoparticles was quietly adding. The total solution was fixed to 3 wt.%. The solution was filled in a 20 ml NORM-JECT Luer Lock tip plastic syringe having an 18-gauge stainless steel needle with 90° blunt end. The electrospinning setup included a high voltage power supply, purchased from the NanoNC, Inc. (S. Korea), and a nanofiber collector of aluminum foil that covered a laboratoryproduced roller with the diameter of 10 cm. The collector was placed at 10 cm

2.6.1 Synthesis of complex nanoparticles by sonochemistry method (green chemistry)

hydroxybenzylidene)-6-(((E)-5-bromo-2-hydroxybenzylidene)amino)-4-oxo-2 thioxo-1,2,3,4-tetrahydrothieno[3,2-d]pyrimidine-7-carbohydrazide H2L<sup>1</sup>

diphenylethan-1-ol H2L<sup>3</sup> was added dropwise. The obtained precipitates were allowed to evaporate at room temperature to obtain the complex nanoparticle in

output of 400 W. Ten milliliter of 0.1 M solution of N'-((E)-5-bromo-2-

Ten milliliter of 0.1 M solution of La(NO3)3.6H2O in ethanol was positioned in a high-density ultrasonic probe, operating at 24 kHz with a maximum power [23]

In the present investigation, the analytical data of the prepared metal complexes

suggest the structures as given in Figure 6. The physicochemical results of the newly synthesized compounds were presented in Table 1. The obtained results

, and 2-((2-mercapto- phenyl) imino)-1,2-

, N-(2-

Dressings play multimodal roles in wound healing process, such as preventing infections, absorbing exudates, and maintaining moisture, and thus are indispensable [17]. Electrospinning is the method to fabricate ultrafine fibers from polymer solution(s) or melt(s) with diameters ranging from nanometers to micrometers [18].

The obtained results revealed the successful implementation of the nanoparticlenanofiber as a local wound dressing especially for skin injuries.
