9. Comparison between IR and other techniques for calculating DD

To differentiate chitin from chitosan, it is necessary to define the degree of acetylation (DA), that is to say the ratio of the number of units comprising an acetyl group on the number of units in the molecule. We can also speak of degree of deacetylation (DD) such that: DD = 100 - DA in%.

The calculation of DD was made according to several methods described in the literature: acid and basic conductometric method, pH-metric method, UV method, and IR spectroscopy method. In a recent study [70], an attempt was made to

compare IR and other analytical methods to compute DD. The analysis was performed by acidic conductometric assay. Basic conductometric dosing and pHmetric dosing techniques will be compared to IR.
