**5.2 French pressure cell method**

It involves the extrusion of multilamellar vesicles (MLV) through a small orifice. The method involves the gentle handling of unstable materials. The resulting liposomes are larger than those generated by using the sonicated method. However, high temperatures are difficult to obtain and working volumes are small.

## **5.3 Freeze-thawed liposome method**

The creation of unilamellar vesicles is a result of a fusion of UV throughout the processes of rapidly freezing and slowly thawing. The encapsulation rates go from 20 to 30%.
