**5. Mechanical dispersion method**

#### **5.1 Handshaking and non-handshaking method**

In order to produce liposomes, lipid molecules must be introduced into an aqueous environment. When a dry lipid layer film is hydrated, lamellae swell and grow

into myelin figures. Mechanical agitation provided by vortexing, shaking, swirling, or pipetting causes myelin figures to break and reseal the exposed hydrophobic edges, resulting in the formation of liposomes, which can be made by using the handshaking method.
