4. Conclusion

Pretreatment is very crucial in the conversion of lignocellulosic materials to other value-added products as lignin acts as the barrier for enzyme penetration. Comparing various pretreatment strategies, fungal pretreatment is more favorable because it is an environmental-friendly process. White-rot fungi with high selectivity of delignification than cellulose removal are more desirable compared to other microorganisms as cellulose is the feedstock for the subsequent hydrolysis process. Fungal strain, inoculum concentration, moisture content, temperature, pH, aeration and supplements are crucial parameters for fungal growth and metabolism to achieve good pretreatment outcome.
