**7.7 Assembly PCR or polymerase cycling assembly (PCA)**

This technique is used for the synthesis of long DNA molecules from long oligonucleotides with short overlapping segments, alternating between sense and antisense directions. The process begins with an initial PCR with primers that have an overlap, followed by a second PCR using the products of the first PCR as the template to generate the final full-length DNA structure [4, 7, 47].
