3.1. Molecular genetics typing of E. faecalis

E. faecalis was tested for genes, coding various pathogenicity factors, using PCR method. It was found that clinical strains (n = 30) of enterococci isolated from urine of children with UTI contained two and more studied pathogenicity genes. In this context, 27 out of 30 uropathogenic E. faecalis had four and more of the studied genes (Table 3).

Eleven variants of genes combinations, which code pathogenicity factors of E. faecalis, were identified. The most common variants are (aggA-esp-cylA-efaA-eep-gelE) and (aggA,efaA,eep, gelE).

Multilocus sequence typing (MLST) divided 17 E. faecalis strains into eight (ST6, ST16, ST21, ST40, ST41, ST116, ST179, and ST774) sequence types (The results have not been published.) It was noticed that uropathogenic E. faecalis strains attributed to ST6, ST40, ST179, ST774, and ST116 are resistant to four and more groups of antimicrobial agents (Table 4).

The results demonstrate broad variability of the range of genes, which code pathogenicity factors and reveal sequence types with multiple resistances to antimicrobial agents among uropathogenic E. faecalis isolated from children with UTI in Primorsky region.
