**Chapter 5**

*Molecular Biotechnology*

[15] Itoh T, Hibi T, Fujii Y, Sugimoto I, Fujiwara A, Suzuki F, et al. Cooperative degradation of chitin by extracellular and cell surface—expressed chitinases from *Paenibacillus* sp. strain FPU-7. Applied and Environmental Microbiology. 2013;**79**:7482-7490. DOI: 10.1128/AEM.02483-13

with high activity toward partially N-acetylated chitosan from a new, moderately thermophilic, chitindegrading bacterium, *Ralstonia* Sp. A-471. Applied Microbiology and Biotechnology. 2004;**63**:398-406. DOI: 10.1007/s00253-003-1351-2

[22] Ueda M, Fujita Y, Kawaguchi T, Arai M. Cloning, nucleotide sequence and expression of the β-*N*-acetylglucosaminidase gene from *Aeromonas* sp. No. l0S-24. Journal of Bioscience and Bioengineering. 2000;**89**:164-169. DOI: 10.1016/S1389-1723(00)88731-9

[23] Vaaje-Kolstad HSV, van

2005;**280**:28492-28497

[24] Wang LX. The amazing

transglycosylation activity of Endoβ-N-acetylglucosaminidases. Trends in Glycoscience and Glycotechnology. 2011;**23**:33-52. DOI: 10.4052/tigg.23.33

Aalten DMF, Synstad B, Eijsink VGH. The non-catalytic chitin-binding protein CBP21 from *Serratia marcescens* is essential for chitin degradation. The Journal of Biological Chemistry.

[16] Shiro M, Ueda M, Kawaguchi T, Arai M. Cloning of a cluster of Chitinase genes from *Aeromonas* sp. No. 10S-24. Biochimica et Biophysica

[17] Arimori T, Kawamoto N, Shinya S, Okazaki N, Nakazawa M, Miyatake K, et al. Crystal structures of the catalytic domain of a novel glycohydrolase family 23 chitinase from *Ralstonia* Sp. A-471 reveals a unique arrangement of the catalytic residues for inverting chitin hydrolysis. The Journal of Biological Chemistry. 2013;**288**:18696-18706

[18] Hashimoto M, Ikegami T, Seino S, Ohuchi N, Fukada H, Sugiyama J, et al. Expression and characterization of the chitin-binding domain of chitinase A1 from *Bacillus circulans* WL-12. Journal of Bacteriology. 2000;**182**:3045-3054. DOI: 10.1128/jb.182.11.3045-3054.2000

[19] Sujatha MS, Sasidhar YU, Balaji PV. Energetics of galactose- and glucosearomatic amino acid interactions: Implications for binding in galactosespecific proteins. Protein Science. 2004;**13**:2502-2514. DOI: 10.1110/

[20] Hara M, Sugimoto H, Uemura M, Akagi K, Suzuki K, Ikegami T, et al. Involvement of Gln679, in addition to Trp687, in chitin-binding activity of the chitin-binding domain of chitinase A1 from *Bacillus circulans* WL-12. Journal of Biochemistry. 2013;**154**:185-193. DOI:

Acta. 1996;**1305**:44-48. DOI: 10.1016/0167-4781(95)00213-8

**86**

ps.04812804

10.1093/jb/mvt043

[21] Sutrisno A, Ueda M, Abe Y, Nakazawa M, Miyatake K. A chitinase
