**3.6 Expression of Pb-LPMO gene and characterization of recombinant protein**

The molecular mass of the recombinant protein rPb-LPMO was 45 kDa, as determined by SDS-PAGE (data not shown), in good agreement with that predicted from the amino acid sequence. The activity of rPb-LPMO was determined in enzyme assays using α-chitin or β-chitin as the substrate. After incubation, oxidized products were detected by HPLC (data not shown).
