**2.6 Optimum ratio of Pb-LPMO, Pb-ChiA, and Pb-GlcNAcase during the hydrolysis of crystalline chitin**

We incubated 3.0 μM Pb-LPMO, 1 μM Pb-ChiA, Pb-GlcNAcase (0, 0.1, 1.0, 3.0, 5.0, and 10 μM), and 1.0 mM ascorbic acid in 20 mM acetate buffer (pH 5.0) with α-chitin at 37°C for 24 h. The reaction mixture was then boiled for 10 min to terminate the enzyme reaction. The samples were mixed with the same volume of acetonitrile. The resultant solution was applied onto the same column and conditions as mentioned in Section 2.4. In this experiment, we estimated the concentration of GlcNAc, (GlcNAc)2, and total sugars (GlcNAc). The concentration of (GlcNAc)2 in total sugars was calculated as the concentration of two GlcNAc.
