**Meet the editors**

Aditi Singh is a research enthusiast, working as an application scientist in a biotechnology company. She has a PhD degree in molecular-cell biology from the University of Heidelberg in Germany and worked as a postdoctoral scholar at the University of California San Diego, USA. Aditi has more than nine years of experience in the field of molecular and cell biology, where she has been involved in various R&D projects and technical support. Her research interests are cell biology, RNA biology, gene editing, and infectious diseases.

Mohammad W. Khan is a research enthusiast, working as an application scientist in a biotechnology company. He has a PhD degree from the University of Heidelberg in Germany and a DVM degree from Maharashtra Animal and Fishery Sciences University, Nagpur, India. He also worked as a postdoctoral scholar at San Diego State University, USA. Dr Khan has more than 15 years of experience in the field of molecular and cell biology, where he has been involved in various R&D projects and technical support. He has authored and reviewed several research articles and has also served as guest editor for several high-impact journals. His research interests are protein biology, RNA biology, cancer research, immunology, genomics, gene editing, and infectious diseases.

Contents

**Preface VII**

Aditi Singh

**Section 2 Gene Silencing Applications 5**

Sanchez-Martin

Amal Qattan

and Moises Zotti

**Nucleic Acids 61**

**Knock-In System 83**

Lin

Chapter 1 **Introductory Chapter: Modulating Gene Expression - Abridging**

Chapter 4 **Nontransformative Strategies for RNAi in Crop Protection 41**

Chapter 5 **Strand Displacement Amplification for Multiplex Detection of**

Chapter 6 **MultiSite Gateway Technology Is Useful for Donor DNA Plasmid Construction in CRISPR/Cas9-Mediated**

Takeshi Yasuda and Katsushi Tajima

Ignacio García-Tuñon, Elena Vuelta, Sandra Pérez-Ramos, Jesús M Hernández-Rivas, Lucía Méndez, María Herrero and Manuel

Deise Cagliari, Ericmar Avila dos Santos, Naymã Dias, Guy Smagghe

Lingwen Zeng, Omar Mukama, Xuewen Lu, Shilin Cao and Donghai

**the RNAi and CRISPR-Cas9 Technologies 3**

Chapter 2 **CRISPR-ERA for Switching Off (Onco) Genes 7**

Chapter 3 **Gene Silencing Agents in Breast Cancer 27**

**Section 3 Novel Gene Silencing Methodologies 59**

**Section 1 Introduction 1**
