Author details

Tatsuaki Tsuruyama\* and Takuya Hieatsuka

\*Address all correspondence to: tsuruyam@kuhp.kyoto-u.ac.jp

Department of Drug and Discovery Medicine, Pathology Division, Graduate School of Medicine, Kyoto University, Kyoto, Japan

[6] Kakimoto Y et al. Novel in situ pretreatment method for significantly enhancing the signal in MALDI-TOF MS of formalin-fixed paraffin-embedded tissue sections. PLoS One. 2012;

Novel Techniques in Histologic Research: Morphometry and Mass Spectrometry Imaging

http://dx.doi.org/10.5772/intechopen.81158

133

[7] Ronci M et al. Protein unlocking procedures of formalin-fixed paraffin-embedded tissues: Application to MALDI-TOF imaging MS investigations. Proteomics. 2008;8:3702-3714.

[8] Gustafsson JO, Oehler MK, McColl SR, Hoffmann P. Citric acid antigen retrieval (CAAR) for tryptic peptide imaging directly on archived formalin-fixed paraffin-embedded tissue.

[9] Bouschen W, Schulz O, Eikel D, Spengler B. Matrix vapor deposition/recrystallization and dedicated spray preparation for high-resolution scanning microprobe matrix-assisted laser desorption/ionization imaging mass spectrometry (SMALDI-MS) of tissue and single cells. Rapid Communications in Mass Spectrometry. 2010;24:355-364. DOI: 10.1002/rcm.4401

[10] Yang J, Caprioli RM. Matrix sublimation/recrystallization for imaging proteins by mass spectrometry at high spatial resolution. Analytical Chemistry. 2011;83:5728-5734. DOI:

[11] Kenyon CN, Melera A, Erni F. Utilization of direct liquid inlet LC/MS in studies of pharmacological and toxicological importance. Journal of Analytical Toxicology. 1981;5:216-230

[12] Kim HY, Salem N Jr. A new technique for lipid analysis using liquid chromatography/mass spectrometry. Advances in Alcohol & Substance Abuse. 1988;7:241-247. DOI: 10.1300/

[13] Ramseier A, Siethoff C, Caslavska J, Thormann W. Confirmation testing of amphetamines and designer drugs in human urine by capillary electrophoresis-ion trap mass spectrometry.

[14] Quanico J, Franck J, Wisztorski M, Salzet M, Fournier I. Combined MALDI mass spectrometry imaging and parafilm-assisted microdissection-based LC-MS/MS workflows in the study of the brain. Methods in Molecular Biology. 2017;1598:269-283. DOI: 10.1007/

[15] Panderi I et al. Differentiating tumor heterogeneity in formalin-fixed paraffin-embedded (FFPE) prostate adenocarcinoma tissues using principal component analysis of matrixassisted laser desorption/ionization imaging mass spectral data. Rapid Communications

[16] Bornsen KO. Influence of salts, buffers, detergents, solvents, and matrices on MALDI-MS protein analysis in complex mixtures. Methods in Molecular Biology. 2000;146:387-404.

[17] Angel PM et al. Mapping extracellular matrix proteins in formalin-fixed, paraffinembedded tissues by MALDI imaging mass spectrometry. Journal of Proteome Research.

in Mass Spectrometry. 2017;31:160-170. DOI: 10.1002/rcm.7776

2018;17:635-646. DOI: 10.1021/acs.jproteome.7b00713

7:e41607. DOI: 10.1371/journal.pone.0041607. PONE-D-12-07910 [pii]

Journal of Proteome Research. 2010;9:4315-4328. DOI: 10.1021/pr9011766

DOI: 10.1002/pmic.200701143

10.1021/ac200998a

J251v07n03\_32

Electrophoresis. 2000;21:380-387

DOI: 10.1385/1-59259-045-4:387

978-1-4939-6952-4\_13
