7. Examination of the sentinel lymph node and dissected material of the neck

The sentinel lymph node was prepared by the pathologist in paraffin blocks with serial cuts at 100–150 microns. With serial cuts, the alternating staining with hematoxylin and eosin and immunocytochemical staining with cytokeratin were used.

As a reagent for immunohistochemical staining, a reagent produced by Dako-Glostrup (monoclonal mouse anti-human cytokeratin clone AE 1/AE 3) was used.

All the remaining removed lymph nodes were examined with the classical methods of staining with hematoxylin and eosin in paraffin blocks with 1–3 slices along the longitudinal axis of the lymph node.
