11. Results from software

All the absorbance readings are fed in the "Software reader for window version 1.51" to collect the results.

### 11.1 Manual measurement of endotoxin

Following Formula is used to calculate the results

Endotoxin=ml in product <sup>¼</sup> conc:of std:in solution � sample ABS � blank ABS Std:ABS � blank ABS � DF

Calculations of MVC, MVD and ELC.

$$\mathbf{M} \mathbf{V} \mathbf{C} = \mathbf{M} / \mathbf{K}$$

Author details

Yasir Mehmood

Pakistan

105

Faculty of Pharmaceutical Sciences, Government College University Faisalabad,

What Is Limulus Amebocyte Lysate (LAL) and Its Applicability in Endotoxin Quantification…

DOI: http://dx.doi.org/10.5772/intechopen.81331

© 2019 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/ by/3.0), which permits unrestricted use, distribution, and reproduction in any medium,

\*Address all correspondence to: yasir\_dpharm@hotmail.com

provided the original work is properly cited.

Where the lowest sensitivity of lysate, M is the maximum dose/kg body weight and K is constant having value equal to 5.

> MVD ¼ concentration of product in 1 ml=MVC ELC ¼ X MVD
