7.6.3 Negative controls

LRW negative controls should be included with each test 1: Equation of straight line (results) y = mx + c m = slop x = endotoxin concentration, c = y-intercept and y = mean absorbance X = y-c/m Example calculation

### 7.6.4 Sample preparation

Prepare sample solutions by dissolving or diluting drugs (pH 6.0–8.0). The pH may be adjusted by the use of acid, base, or suitable buffers as recommended. Do not exceed the MVD or MCV while making dilutions and adjusting the pH.
