**3.5 Preparation of a glycerol stock**


#### **3.6 Plasmid DNA purification**

1.Preheat the TE Buffer in the incubator at 37°C.

<sup>4</sup> For some applications (especially culturing cells in minimal defined media) cultures should never be overgrown; growing overnight cultures at a reduced temperature, 25–30°C, is suggested.

<sup>5</sup> Snap top tubes are not recommended.

