*3.1.9. Manufacturer guidelines*

The company's manufacturing guidelines provide valuable information regarding vaccine efficacy, usage, storage and route. The guidelines are: (1) open the vaccine vial in water and (2) use one complete vial after opening it. The vaccines must be utilized as early as possible after its reconstitution in diluents, etc. Once the vaccine is reconstituted, the time limit is set. Vaccines must be used within 2 h of their reconstitution during winter and within 1 h of their reconstitution during summer. IB vaccines lose potency after 1 h of their reconstitution, while pox vaccines lose 50% of their potency after 1 h of their reconstitution [32].
