**2.2. Experimental design and procedure**

*Macrobrachium vollenhenvonii* juveniles (mean weight = 18.02 ± 0.11 g; n = 360; 180 prawns for each pathogen) were obtained from a reputable farm in Melbourne, Australia and acclimatised for 2 weeks in glass aquaria tanks before the experiment. Prawns were weighed and distributed into 18 glass aquaria tanks (35 × 30 × 20 cm**<sup>3</sup>** ) in a completely randomised design with three replicates. Each tank contained 20 prawns. The tanks were constantly connected to aerator (Model: AP-60) with air blowers. The prawns that were fed experimental diets were further subjected to *Pseudomonas aeruginosa* and *Aeromonas hydrophila* infection for 2 weeks to examine their innate immune performances and survival. The diets were fed to the *Macrobrachium vollenhovenii* to satiation throughout the experimentation. Measurements of the weight changes were measured using sensitive scale (**Model: M1207)**.
