4. Advanced platelet-rich fibrin (A-PRF)

The amount of WBCs effect vascularization and bone remodeling. As a result, researchers made new modifications in the centrifugation speeds and times to prevent cell loss within the PRF matrix [8]. These recent modifications of the PRF protocol have led to the improvement of advanced platelet-rich fibrin (A-PRF), which uses lower G-forces to gain higher growth factors compared to PRF [9]. It maintains higher amount of WBCs in the fibrin matrix and has special glass tubes, which are designed to make clotting faster. After the centrifugation, the tubes are removed and placed in their holders and left for 5 min to start clot formation. The fibrin clot which is rich with WBCs provides higher growth factors and recent research emphasizes that A-PRF enhances collagen matrix synthesis and supplement of progenitor cells [8].
