**5.3 Analysis of cell death by Hoechst 33342 staining**

Chromatin condensation will be assessed by nuclear staining with Hoechst 33342 [24]. Briefly, cells will be seeded in a 96 well plate at a density of 5 × 105 cells and then treated with different concentrations of compounds for 24 h. After washing once with PBS, the cells will be stained with 100 μl of Hoechst 33342 (10 mg/ ml stock) and incubate at room temperature for 5 min. Stained cells can be imaged by fluorescence microscope at 350–460 nm compound induced in KB human oral cancer cells for 24 h, stained with Hoechst 33342 (**Figure 6**).
