Nocturnal Enuresis in Sickle Cell Anemia

**11**

**Chapter 2**

**Abstract**

*and Ikenna K. Ndu*

Nocturnal Enuresis in Children

*Samuel N. Uwaezuoke, Chizoma I. Eneh, Osita U. Ezenwosu* 

Sickle cell anemia (SCA) is the commonest hemoglobin disorder among the black population worldwide. Children with SCA may eventually end up with end-organ complications: the kidneys being one of the most frequently affected organs. The renal complications arise from medullary ischemia and infarction leading to features of tubular dysfunction such as hyposthenuria and renal tubular acidosis. Early in life, children with SCA may present with hyposthenuria: one of the earliest renal defects in the disease which results in an obligatory urine output of more than 2 l in a day. The symptomatic manifestation as nocturnal polyuria is thought to be the reason for nocturnal enuresis observed in these children. In spite of the more prevalent occurrence of nocturnal enuresis in children with SCA than in their non-SCA colleagues, its precise underlying mechanisms still remain controversial, with divergent conclusions regarding its pathogenesis. However, the consensus is now tilting towards a multifactorial etiopathogenesis in affected children. This book chapter aims to discuss the epidemiologic perspectives of nocturnal enuresis in SCA, as well as the current

with Sickle Cell Anemia

hypotheses on the etiopathogenesis of this complication.

SCA are essentially related to these two events.

multifactorial etiopathogenesis

**1. Introduction**

**Keywords:** sickle cell anemia, nocturnal enuresis, hyposthenuria,

Sickle cell anemia (SCA) is the commonest hemoglobin disorder among the black population worldwide [1, 2]. As a genetic defect with the Mendelian autosomal-recessive inheritance, children with the sickle cell hemoglobin genes in the homozygous form have reversibly sickled and irreversibly sickled red blood cells. These abnormal red cells, which become rigid having lost their deformability, consequently block the microvasculature resulting in vasoocclusion. They are also prone to damage which leads to chronic hemolysis. Most of the clinical features of

Children with SCA may eventually end up with end-organ complications: the kidneys being one of the most frequently affected organs. The renal complications arise from medullary ischemia and infarction leading to features of tubular dysfunction such as hyposthenuria and renal tubular acidosis [3]. As early as 3 years of age, children with SCA may present with hyposthenuria: one of the earliest renal defects in the disease which results in an obligatory urine output of more than 2 l in a day [4]. The symptomatic manifestation as nocturnal polyuria is thought to

### **Chapter 2**

## Nocturnal Enuresis in Children with Sickle Cell Anemia

*Samuel N. Uwaezuoke, Chizoma I. Eneh, Osita U. Ezenwosu and Ikenna K. Ndu*

### **Abstract**

Sickle cell anemia (SCA) is the commonest hemoglobin disorder among the black population worldwide. Children with SCA may eventually end up with end-organ complications: the kidneys being one of the most frequently affected organs. The renal complications arise from medullary ischemia and infarction leading to features of tubular dysfunction such as hyposthenuria and renal tubular acidosis. Early in life, children with SCA may present with hyposthenuria: one of the earliest renal defects in the disease which results in an obligatory urine output of more than 2 l in a day. The symptomatic manifestation as nocturnal polyuria is thought to be the reason for nocturnal enuresis observed in these children. In spite of the more prevalent occurrence of nocturnal enuresis in children with SCA than in their non-SCA colleagues, its precise underlying mechanisms still remain controversial, with divergent conclusions regarding its pathogenesis. However, the consensus is now tilting towards a multifactorial etiopathogenesis in affected children. This book chapter aims to discuss the epidemiologic perspectives of nocturnal enuresis in SCA, as well as the current hypotheses on the etiopathogenesis of this complication.

**Keywords:** sickle cell anemia, nocturnal enuresis, hyposthenuria, multifactorial etiopathogenesis

### **1. Introduction**

Sickle cell anemia (SCA) is the commonest hemoglobin disorder among the black population worldwide [1, 2]. As a genetic defect with the Mendelian autosomal-recessive inheritance, children with the sickle cell hemoglobin genes in the homozygous form have reversibly sickled and irreversibly sickled red blood cells. These abnormal red cells, which become rigid having lost their deformability, consequently block the microvasculature resulting in vasoocclusion. They are also prone to damage which leads to chronic hemolysis. Most of the clinical features of SCA are essentially related to these two events.

Children with SCA may eventually end up with end-organ complications: the kidneys being one of the most frequently affected organs. The renal complications arise from medullary ischemia and infarction leading to features of tubular dysfunction such as hyposthenuria and renal tubular acidosis [3]. As early as 3 years of age, children with SCA may present with hyposthenuria: one of the earliest renal defects in the disease which results in an obligatory urine output of more than 2 l in a day [4]. The symptomatic manifestation as nocturnal polyuria is thought to

be the reason for the observed nocturnal enuresis in these children. In spite of the more prevalent occurrence of nocturnal enuresis in children with SCA than in their normal colleagues, the precise underlying mechanisms have not yet been resolved. Research on the subject has led to divergent conclusions about the pathogenesis; one report had earlier suggested that hyposthenuria was a major determinant of enuresis in the disease [5], while other authors not only controverted this observation but had reported disparate etiopathogenic factors [6–8]. In fact, a recent review of published evidence on the subject indicates similar determinants of nocturnal enuresis for both SCA and non-SCA patients [9]. Thus, the role of hyposthenuria as the exclusive determinant of nocturnal enuresis in children with SCA remains debatable although the consensus is now tilting towards a multifactorial etiopathogenesis in affected children.

This book chapter aims to discuss the epidemiologic perspectives of nocturnal enuresis in SCA, as well as the current hypotheses on the etiopathogenesis of this complication.

### **2. Nocturnal enuresis in SCA: epidemiologic perspectives**

Nocturnal enuresis has been defined as the persistence of urination in the bed (bedwetting) at night, two or more times per week after the age of 5 years, for a period of at least 3 months [10]. It can present as a primary form (no previous dry period) or a secondary form (previous dry period), and as monosymptomatic (absence of daytime symptoms) or non-monosymptomatic (presence of daytime symptoms) [9]. Studies which show that children with SCA have a tendency for nocturnal enuresis more than children with normal hemoglobin however reported different prevalence rates and epidemiologic patterns, depending on study methods and definition criteria (**Table 1**).

#### **2.1 Presumed risk factors for nocturnal enuresis**

For instance, the possible effect of sex and socioeconomic status on enuresis in children has been well documented in several studies [7, 11–16]. Firstly, male predominance was noted among children with SCA in some of the studies [7, 11, 13–15], whereas a female predominance was reported in one study [12]. Although the disparity could be due to study selection bias, a similar trend of male predominance has also been reported among non-SCA children [15, 17]. This gender bias suggests that contributory factors to nocturnal enuresis in non-SCA children such as slower maturation and reduced responsiveness to toilet training in boys [18], and more frequent developmental delays [19], may also apply to children with SCA. Secondly, there appears to be no significant impact of socioeconomic status on the prevalence of nocturnal enuresis in both SCA children [7, 14], and their non-SCA counterparts [16]. However, a previous report indicates that enuresis in non-SCA children was more frequent in those from lower socioeconomic classes [17], whereas another study noted a higher prevalence among non-SCA children from higher socioeconomic classes [20].

#### **2.2 Global prevalence rates of nocturnal enuresis**

There is a wide variation in the global prevalence rates of nocturnal enuresis among children with SCA (**Table 1**). Prevalence rates vary from 25–51% depending on methodology and definition of nocturnal enuresis adopted in each study. In the West African sub-region, prevalence rates of 41.6, 31.4 and 47.1% were reported in south-west [11], south-east [14], and north-west [15] regions of Nigeria respectively.

**13**

**Table 1.**

*‡*

*Nocturnal Enuresis in Children with Sickle Cell Anemia DOI: http://dx.doi.org/10.5772/intechopen.81578*

> **Study method (age bracket)**

Prospective parental interview (5–11 years)

Prospective parental interview (4–20 years)

Prospective parental interview (5–16 years)

Crosssectional study (5–20 years)

Prospective questionnaire (5–17 years)

Prospective phone interview (5–22 years)

Prospective interview (5–17 years)

Prospective screening questionnaire (6–21 years)

Prospective questionnaire (6–20 years)

Prospective questionnaire (4–19 years)

Prospective interview (8 years)

Prospective questionnaire (6–40 years)

**NE definition Prevalence** 

Involuntary passage of urine during sleep>1/ month

≥3 bedwetting episodes/ month (5–6 year old) or 1 episode/ month (>6 year old)

Micturition during sleep in a child aged >5 years

Nocturnal urinary incontinence >5 years of age (>2/week for 3 months)

Urinary incontinence >5 years of age (>2/week for 3 months)

Bed wetting at least 2/week

Recurrent bed wetting

Pre-sleep bed wetting/month

Bed wetting 2 nights/week

Bed wetting at night >1/week for 3 months

*N = study population, DSM-IV = Diagnostic and Statistical Manual of Mental Disorders, Fourth edition,* 

*South East Nigeria.*

*Nocturnal enuresis (NE) in children with sickle cell anemia: epidemiologic perspectives.*

**rates (N)**

DSM-IV criteria 31.4% (70) Male Not significant

**Sex predominance**

41.6% (209) Male Not reported

47.1% (360) Male Not significant

51% (456) Female Not reported

39.2% (217) Male Not reported

25% (126) Not reported Not reported

30% (91) Not reported Not reported

33% (213) Not reported Not reported

39% (221) Not reported Not reported

45% (175) Male Not significant

26.4% (55) Not reported Not reported

Not stated 32% (155) Not reported Not reported

**Effect of socioeconomic status**

**Study authors (Country)**

Eneh et al. (Nigeria)†

Akinyanju et al. (Nigeria)‡

Ogunrinde et al. (Nigeria)††

Mabiala Babela et al. (Congo Brazzaville)

Portocarrero et al. (Brazil)

Barakat et al. (USA)

Jordan et al. (USA)

Figueroa et al. (USA)

Field et al. (USA)

Lehmann et al. (USA)

Readett et al. (Jamaica)

Ekinҫi et al. (Turkey)

*South West Nigeria. ††North West Nigeria.*

*USA = United States of America.†*


*Contemporary Pediatric Hematology and Oncology*

genesis in affected children.

and definition criteria (**Table 1**).

**2.1 Presumed risk factors for nocturnal enuresis**

**2.2 Global prevalence rates of nocturnal enuresis**

complication.

be the reason for the observed nocturnal enuresis in these children. In spite of the more prevalent occurrence of nocturnal enuresis in children with SCA than in their normal colleagues, the precise underlying mechanisms have not yet been resolved. Research on the subject has led to divergent conclusions about the pathogenesis; one report had earlier suggested that hyposthenuria was a major determinant of enuresis in the disease [5], while other authors not only controverted this observation but had reported disparate etiopathogenic factors [6–8]. In fact, a recent review of published evidence on the subject indicates similar determinants of nocturnal enuresis for both SCA and non-SCA patients [9]. Thus, the role of hyposthenuria as the exclusive determinant of nocturnal enuresis in children with SCA remains debatable although the consensus is now tilting towards a multifactorial etiopatho-

This book chapter aims to discuss the epidemiologic perspectives of nocturnal enuresis in SCA, as well as the current hypotheses on the etiopathogenesis of this

Nocturnal enuresis has been defined as the persistence of urination in the bed (bedwetting) at night, two or more times per week after the age of 5 years, for a period of at least 3 months [10]. It can present as a primary form (no previous dry period) or a secondary form (previous dry period), and as monosymptomatic (absence of daytime symptoms) or non-monosymptomatic (presence of daytime symptoms) [9]. Studies which show that children with SCA have a tendency for nocturnal enuresis more than children with normal hemoglobin however reported different prevalence rates and epidemiologic patterns, depending on study methods

For instance, the possible effect of sex and socioeconomic status on enuresis in children has been well documented in several studies [7, 11–16]. Firstly, male predominance was noted among children with SCA in some of the studies [7, 11, 13–15], whereas a female predominance was reported in one study [12]. Although the disparity could be due to study selection bias, a similar trend of male predominance has also been reported among non-SCA children [15, 17]. This gender bias suggests that contributory factors to nocturnal enuresis in non-SCA children such as slower maturation and reduced responsiveness to toilet training in boys [18], and more frequent developmental delays [19], may also apply to children with SCA. Secondly, there appears to be no significant impact of socioeconomic status on the prevalence of nocturnal enuresis in both SCA children [7, 14], and their non-SCA counterparts [16]. However, a previous report indicates that enuresis in non-SCA children was more frequent in those from lower socioeconomic classes [17], whereas another study noted a higher prevalence among non-SCA children from higher socioeconomic classes [20].

There is a wide variation in the global prevalence rates of nocturnal enuresis among children with SCA (**Table 1**). Prevalence rates vary from 25–51% depending on methodology and definition of nocturnal enuresis adopted in each study. In the West African sub-region, prevalence rates of 41.6, 31.4 and 47.1% were reported in south-west [11], south-east [14], and north-west [15] regions of Nigeria respectively.

**2. Nocturnal enuresis in SCA: epidemiologic perspectives**

**12**

*N = study population, DSM-IV = Diagnostic and Statistical Manual of Mental Disorders, Fourth edition, USA = United States of America.† South East Nigeria.*

*‡ South West Nigeria.*

*††North West Nigeria.*

#### **Table 1.**

*Nocturnal enuresis (NE) in children with sickle cell anemia: epidemiologic perspectives.*

The study in south-east of the country prospectively interviewed parents of SCA subjects aged 5–11 years and parents of age- and sex-matched non-SCA controls; using the Diagnostic and Statistical Manual of Mental Disorders, Fourth edition (DSM-IV) criteria to define nocturnal enuresis [14]. In South West Nigeria, the authors also used prospective parental interview for subjects and controls aged 4–20 years, but defined nocturnal enuresis as 'involuntary micturition during sleep which occurred more often than once in a month' [11]. Conversely, the study in North West Nigeria used a structured questionnaire to obtain information from parents of enrolled 5- to 16-year-old subjects and controls; defining nocturnal enuresis as '3 or more episodes of bedwetting per month in a child aged 5 to 6 years and at least, once monthly in an older child' [15]. In Central Africa, a prevalence rate of 51% was reported in a cross-sectional study of 5- to 20-year-old SCA patients (with age- and sex-matched controls) in Congo-Brazzaville which used the defining criteria for nocturnal enuresis as 'complete act of urination most often during sleep in a child over 5 years' [12]. Elsewhere in South America, a Brazilian study which was conducted on 5- to 17-year-old Negroid children and adolescents with SCA reported a prevalence rate of 32% [21]. The authors prospectively administered a questionnaire on the caregivers of these subjects and their age-matched controls. In North America, studies in the United States conducted among African-Americans documented prevalence rates of 39% [6], 39.2% [13], 25% [22], 30% [23], and 33% [24]. In these studies, there was disparity in the age bracket of the study population and the definition adopted for nocturnal enuresis. A prospective phone interview was used in one study, which defined nocturnal enuresis as 'incontinence of urine at night after 5 years of age, more than twice a week for at least 3 months,' while the study population were aged 5–22 years [13]. Another study employed a prospective interview of parents whose children were aged 5–17 years, and defined nocturnal enuresis as 'incontinence of urine at night after 5 years of age, more than twice a week for at least 3 months' [22]. Two of the studies utilized prospective questionnaires [6, 24], but interviewed primary caregivers of subjects who were aged 6–20 years [6], and 4–19 years [24]; defining nocturnal enuresis as 'recurrent problem with bedwetting' [6], and 'wet bed in 1 month before sleep study' [24]. In the last of the studies, nocturnal enuresis was defined 'as wetting bed at least twice per week' [23]; primary nocturnal enuresis was studied among subjects aged 6–21 years with a prospective screening questionnaire. In the Caribbean, a Jamaican study which used the prospective interview method reported a prevalence rate of 45% among 8-year-old SCA patients [7]. The authors' definition of nocturnal enuresis was 'being wet for at least 2 nights per week.' A study in Turkey which adopted the definition criterion of nocturnal enuresis as 'wet bed at night more than once a week for at least 3 months,' reported a prevalence rate of 26.4% [25]. The investigators conducted semi-structured interviews with caregivers of pediatric and adult patients. Perhaps, the combination of SCA and thalassemia patients in the study population (with preponderance of the latter) as well as the wide age-bracket of 6–40 years accounted for this comparatively lower prevalence rate. Furthermore, it has been established that the prevalence of nocturnal enuresis decreases with advancing age, although this finding was essentially noted among non-SCA subjects [26, 27].

#### **3. Nocturnal enuresis in SCA: hypotheses on etiopathogenesis**

There are now several hypotheses on the etiopathogenesis of enuresis in children [9, 28, 29]. In fact, it is believed that children with SCA may have a tendency to develop nocturnal enuresis because of the common general etiopathogenic factors in childhood, SCA-related etiopathogenic factors or a combination of both [9]. Specifically, the unresolved questions include the following: What is the exact

**15**

*Nocturnal Enuresis in Children with Sickle Cell Anemia DOI: http://dx.doi.org/10.5772/intechopen.81578*

nocturnal enuresis revolve around these posers.

role of hyposthenuria in SCA-related nocturnal enuresis? What are the contributory bladder-specific factors? Is there any relationship between sleep disordered breathing (SDB) and nocturnal enuresis in SCA? and Is there a difference in arousability threshold in normal subjects with nocturnal enuresis and those with SCA? [9]. Interestingly, the current hypotheses on the etiopathogenesis of SCA-related

Firstly, the nocturnal polyuria resulting from hyposthenuria has long been suggested as the cause of nocturnal enuresis in SCA patients [5]. This hypothesis is supported by the fact that hyposthenuria is one of the commonest and earliest infarction-related renal complications, as intravascular sickling occurs more readily in the kidneys than in any other organs [30]. The microvasculature of the renal medulla is particularly susceptible to hypoxia induced by sickling and vasoocclusion [31]. Medullary ischemia and infarction result in the impairment of the urine-concentrating ability of the vasa recta and juxtamedullary nephrons, as failure of this function is thought to manifest as polyuria and enuresis [32, 33]. Although the 'hyposthenuria hypothesis' has been disputed by some authors who failed to establish a causal link between SCA and enuresis [7, 34], it has later been observed that both urine osmolality and overnight urine volume after fluid restriction were similar in enuretic and non-enuretic children with SCA; making the authors to conclude that low maximum functional bladder capacity and high overnight urine volume to maximum functional bladder capacity ratio were the determinants of nocturnal enuresis in affected children

rather than low urine osmolality and high overnight urine volume [8].

Secondly, another hypothesis on nocturnal enuresis in the general population is that it results from an interaction of detrusor instability, delayed arousal from sleep and nocturnal polyuria [28]. Other authors also observed that in enuretic children, the nocturnal bladder capacity during sleep was significantly smaller than the diurnal functional capacity; thus highlighting the role of the inability to hold urine during sleep as an important etiopathogenic mechanism for nocturnal enuresis [29]. Nocturnal polyuria, nocturnal detrusor over-activity and high arousal thresholds are now regarded as crucial factors in the pathogenesis of enuresis, with an underlying mechanism on the brainstem level probably common to these pathogenic mechanisms [35]. In a review which appraised the possible etiopathogenic factors of primary nocturnal enuresis, the partly proven mechanisms were listed as maturational delay of the central nervous system, genetic factors, sleep disorders and SDB, and low levels of nocturnal anti-diuretic hormone (ADH) secretion [36]. Among these hypotheses, delayed functional maturation of the central nervous system is thought to be the most plausible mechanism for nocturnal enuresis as it reduces the child's ability to inhibit nocturnal bladder emptying [36]. This theory is supported by the observation of spontaneous improvement in enuresis which occurs with advancing age [26]. Despite bladder filling, the non-perception of the sensory output emanating from its stretching removes the cortical control on the contraction of the urethral sphincter. Failure of the sleep arousal mechanism due to high arousal thresholds may also contribute to this inability to inhibit nocturnal bladder emptying. Presumably, these hypotheses on etiopathogenesis also apply to nocturnal enuresis in children with SCA. For instance, in these children a strong link between nocturnal enuresis and urinary bladder dysfunction has been reported by several authors [8, 37, 38]. Another recent postulation is that SCA-related enuresis may be due to atonic detrusor muscle which results in an underactive bladder with defective emptying mechanism. This abnormality is thought to be a consequence of chronic bladder ischemia caused by recurrent cycles of ischemia-perfusion injury triggered by vasoocclusion [39]. Evidence for this hypothesis was reported by some authors who studied the urinary bladder function in a transgenic sickle cell disease murine model and found these pathophysiologic changes: reduced urine output, inability

#### *Nocturnal Enuresis in Children with Sickle Cell Anemia DOI: http://dx.doi.org/10.5772/intechopen.81578*

*Contemporary Pediatric Hematology and Oncology*

The study in south-east of the country prospectively interviewed parents of SCA subjects aged 5–11 years and parents of age- and sex-matched non-SCA controls; using the Diagnostic and Statistical Manual of Mental Disorders, Fourth edition (DSM-IV) criteria to define nocturnal enuresis [14]. In South West Nigeria, the authors also used prospective parental interview for subjects and controls aged 4–20 years, but defined nocturnal enuresis as 'involuntary micturition during sleep which occurred more often than once in a month' [11]. Conversely, the study in North West Nigeria used a structured questionnaire to obtain information from parents of enrolled 5- to 16-year-old subjects and controls; defining nocturnal enuresis as '3 or more episodes of bedwetting per month in a child aged 5 to 6 years and at least, once monthly in an older child' [15]. In Central Africa, a prevalence rate of 51% was reported in a cross-sectional study of 5- to 20-year-old SCA patients (with age- and sex-matched controls) in Congo-Brazzaville which used the defining criteria for nocturnal enuresis as 'complete act of urination most often during sleep in a child over 5 years' [12]. Elsewhere in South America, a Brazilian study which was conducted on 5- to 17-year-old Negroid children and adolescents with SCA reported a prevalence rate of 32% [21]. The authors prospectively administered a questionnaire on the caregivers of these subjects and their age-matched controls. In North America, studies in the United States conducted among African-Americans documented prevalence rates of 39% [6], 39.2% [13], 25% [22], 30% [23], and 33% [24]. In these studies, there was disparity in the age bracket of the study population and the definition adopted for nocturnal enuresis. A prospective phone interview was used in one study, which defined nocturnal enuresis as 'incontinence of urine at night after 5 years of age, more than twice a week for at least 3 months,' while the study population were aged 5–22 years [13]. Another study employed a prospective interview of parents whose children were aged 5–17 years, and defined nocturnal enuresis as 'incontinence of urine at night after 5 years of age, more than twice a week for at least 3 months' [22]. Two of the studies utilized prospective questionnaires [6, 24], but interviewed primary caregivers of subjects who were aged 6–20 years [6], and 4–19 years [24]; defining nocturnal enuresis as 'recurrent problem with bedwetting' [6], and 'wet bed in 1 month before sleep study' [24]. In the last of the studies, nocturnal enuresis was defined 'as wetting bed at least twice per week' [23]; primary nocturnal enuresis was studied among subjects aged 6–21 years with a prospective screening questionnaire. In the Caribbean, a Jamaican study which used the prospective interview method reported a prevalence rate of 45% among 8-year-old SCA patients [7]. The authors' definition of nocturnal enuresis was 'being wet for at least 2 nights per week.' A study in Turkey which adopted the definition criterion of nocturnal enuresis as 'wet bed at night more than once a week for at least 3 months,' reported a prevalence rate of 26.4% [25]. The investigators conducted semi-structured interviews with caregivers of pediatric and adult patients. Perhaps, the combination of SCA and thalassemia patients in the study population (with preponderance of the latter) as well as the wide age-bracket of 6–40 years accounted for this comparatively lower prevalence rate. Furthermore, it has been established that the prevalence of nocturnal enuresis decreases with advancing age, although this finding was essentially

**14**

noted among non-SCA subjects [26, 27].

**3. Nocturnal enuresis in SCA: hypotheses on etiopathogenesis**

There are now several hypotheses on the etiopathogenesis of enuresis in children

[9, 28, 29]. In fact, it is believed that children with SCA may have a tendency to develop nocturnal enuresis because of the common general etiopathogenic factors in childhood, SCA-related etiopathogenic factors or a combination of both [9]. Specifically, the unresolved questions include the following: What is the exact role of hyposthenuria in SCA-related nocturnal enuresis? What are the contributory bladder-specific factors? Is there any relationship between sleep disordered breathing (SDB) and nocturnal enuresis in SCA? and Is there a difference in arousability threshold in normal subjects with nocturnal enuresis and those with SCA? [9]. Interestingly, the current hypotheses on the etiopathogenesis of SCA-related nocturnal enuresis revolve around these posers.

Firstly, the nocturnal polyuria resulting from hyposthenuria has long been suggested as the cause of nocturnal enuresis in SCA patients [5]. This hypothesis is supported by the fact that hyposthenuria is one of the commonest and earliest infarction-related renal complications, as intravascular sickling occurs more readily in the kidneys than in any other organs [30]. The microvasculature of the renal medulla is particularly susceptible to hypoxia induced by sickling and vasoocclusion [31]. Medullary ischemia and infarction result in the impairment of the urine-concentrating ability of the vasa recta and juxtamedullary nephrons, as failure of this function is thought to manifest as polyuria and enuresis [32, 33]. Although the 'hyposthenuria hypothesis' has been disputed by some authors who failed to establish a causal link between SCA and enuresis [7, 34], it has later been observed that both urine osmolality and overnight urine volume after fluid restriction were similar in enuretic and non-enuretic children with SCA; making the authors to conclude that low maximum functional bladder capacity and high overnight urine volume to maximum functional bladder capacity ratio were the determinants of nocturnal enuresis in affected children rather than low urine osmolality and high overnight urine volume [8].

Secondly, another hypothesis on nocturnal enuresis in the general population is that it results from an interaction of detrusor instability, delayed arousal from sleep and nocturnal polyuria [28]. Other authors also observed that in enuretic children, the nocturnal bladder capacity during sleep was significantly smaller than the diurnal functional capacity; thus highlighting the role of the inability to hold urine during sleep as an important etiopathogenic mechanism for nocturnal enuresis [29]. Nocturnal polyuria, nocturnal detrusor over-activity and high arousal thresholds are now regarded as crucial factors in the pathogenesis of enuresis, with an underlying mechanism on the brainstem level probably common to these pathogenic mechanisms [35]. In a review which appraised the possible etiopathogenic factors of primary nocturnal enuresis, the partly proven mechanisms were listed as maturational delay of the central nervous system, genetic factors, sleep disorders and SDB, and low levels of nocturnal anti-diuretic hormone (ADH) secretion [36]. Among these hypotheses, delayed functional maturation of the central nervous system is thought to be the most plausible mechanism for nocturnal enuresis as it reduces the child's ability to inhibit nocturnal bladder emptying [36]. This theory is supported by the observation of spontaneous improvement in enuresis which occurs with advancing age [26]. Despite bladder filling, the non-perception of the sensory output emanating from its stretching removes the cortical control on the contraction of the urethral sphincter. Failure of the sleep arousal mechanism due to high arousal thresholds may also contribute to this inability to inhibit nocturnal bladder emptying. Presumably, these hypotheses on etiopathogenesis also apply to nocturnal enuresis in children with SCA. For instance, in these children a strong link between nocturnal enuresis and urinary bladder dysfunction has been reported by several authors [8, 37, 38]. Another recent postulation is that SCA-related enuresis may be due to atonic detrusor muscle which results in an underactive bladder with defective emptying mechanism. This abnormality is thought to be a consequence of chronic bladder ischemia caused by recurrent cycles of ischemia-perfusion injury triggered by vasoocclusion [39]. Evidence for this hypothesis was reported by some authors who studied the urinary bladder function in a transgenic sickle cell disease murine model and found these pathophysiologic changes: reduced urine output, inability

to produce the typical bladder contraction and emptying, lower detrusor muscle, small bladder contraction and reduced urethral contraction [40].

Thirdly, the role of sleep disorders and SDB in the etiopathogenesis of nocturnal enuresis has also been advanced as a sleep-related study show that patients with nocturnal enuresis have difficulties in waking, and are thus considered as 'deep sleepers' [41]. In addition, nocturnal enuresis is associated with SDB as a result of upper airway obstruction in children; surgical relief by tonsillectomy, adenoidectomy or both was reported to have reduced nocturnal enuresis in up to 76% of patients [42]. In a recent study, enuresis has not only been linked to SDB in children with SCA but the severity of SDB has been observed to have a strong correlation with frequency of nocturnal enuresis [6]. Notably, SDB is a common sleep disorder comprising a spectrum from snoring to obstructive sleep apnea syndrome (OSAS) which may worsen nocturnal enuresis through disrupted sleep and neurologic dysregulation [9]. While the finding of a study suggests that SDB is more prevalent in children with SCA than in the general population [43], overwhelming evidence also shows that a significant relationship exists between SDB and nocturnal enuresis among non-SCA children [44–46].

Furthermore, the role of low levels of some vitamins in the etiopathogenesis of nocturnal enuresis has been highlighted in recent studies. For instance, enuretic children were observed to have lower serum vitamin B12 and folate levels than their non-enuretic counterparts [47, 48]. The reduced vitamin levels are believed to be associated with slow cortical maturation which has been linked to enuresis. Interestingly, significantly lower or deficient vitamin B12 levels have equally been reported in children with SCA compared to non-SCA controls [49, 50]. In addition, increased risk of nocturnal enuresis has been observed in vitamin D-deficient children as this vitamin deficiency directly correlated with severity of enuresis [51]. In a recent systematic review, the prevalence of vitamin D deficiency was reported to vary from 56.4% to 96.4% in children with SCA [52]. This link between vitamin D deficiency and nocturnal enuresis can be explained partly by its influence on SDB and nocturnal polyuria. Reports indicate that low level of serum 25 (OH) D was associated with increased risk of developing OSAS [53], as well as primary snoring [54]. The association of low vitamin levels with OSAS is reportedly mediated through promotion of adenotonsillar hypertrophy, chronic rhinitis and/or myopathy of airway muscle [55]. Better still, low vitamin D levels may result in nocturnal enuresis through obstructive sleep apnea, sleep fragmentation and nocturnal polyuria, which all occur in children with SCA [39].

Another etiologic consideration for nocturnal enuresis seen in SCA is its association with some involuntary movements such as periodic limb movement syndrome and restless leg syndrome. The prevalence of periodic limb movement syndrome in SCA has been documented as 20.5–29% [56–58], which was significantly higher than the rates of 1.2–8% reported for non-SCA children [59, 60]. Similarly, a prevalence rate of 11.1% has been observed for children with restless leg syndrome [56]. Notably, both involuntary movements are associated with sleep disruption [60]. Given that enuretic children have higher incidence of periodic limb movement and sleep fragmentation [59] and the higher rate of periodic limb movement syndrome associated nocturnal enuresis and sleep disruption in SCA patients, it is therefore not surprising to observe a high prevalence rate of nocturnal enuresis in them. To underscore the nexus between these aforementioned etiologic factors (low serum vitamin D level and restless leg syndrome), it has been observed that Vitamin D supplementation also improved the severity of this involuntary movement [61].

In summary, the etiopathogenic mechanisms involved in nocturnal enuresis among SCA and non-SCA children are multifactorial and not mutually exclusive, and they include hyposthenuria-related nocturnal polyuria, decreased bladder capacity or nocturnal bladder over-activity, high sleep arousal thresholds and SDB [9] (**Figure 1**).

**17**

**Disclosure**

**4. Conclusion**

**Figure 1.**

nocturnal enuresis in children with SCA.

Uwaezuoke et al. [62] and Wolf et al. [9].

The authors declare that there are no conflicts of interest.

**Acknowledgements**

Although nocturnal enuresis appears more prevalent in children with SCA than in their non-SCA counterparts, the exact etiopathogenesis of enuresis is not completely understood. In fact, the suggested mechanisms for nocturnal enuresis in SCA children are also applicable to their non-SCA counterparts. Moreover, the multiregional variations in prevalence rates may be due to differences in definition criteria and study methods. Male predominance in enuretic children has been somewhat established, but there is no unanimity yet on the influence of socioeconomic status on prevalence rates. Perhaps, adopting standardized definitions and study methods may in future minimize the disparities in the reported prevalence rates. More importantly, further research is still required to establish the precise etiopathogenesis of

*The proposed etiopathogenic mechanisms for nocturnal enuresis. SDB, sleep-disordered breathing; nocturnal* 

*polyuria, induced by hyposthenuria. Concept and design: by SNU (one of the authors).*

The authors acknowledge the invaluable information obtained from the articles

*Nocturnal Enuresis in Children with Sickle Cell Anemia DOI: http://dx.doi.org/10.5772/intechopen.81578*

*Nocturnal Enuresis in Children with Sickle Cell Anemia DOI: http://dx.doi.org/10.5772/intechopen.81578*

**Figure 1.**

*Contemporary Pediatric Hematology and Oncology*

all occur in children with SCA [39].

to produce the typical bladder contraction and emptying, lower detrusor muscle,

Thirdly, the role of sleep disorders and SDB in the etiopathogenesis of nocturnal enuresis has also been advanced as a sleep-related study show that patients with nocturnal enuresis have difficulties in waking, and are thus considered as 'deep sleepers' [41]. In addition, nocturnal enuresis is associated with SDB as a result of upper airway obstruction in children; surgical relief by tonsillectomy, adenoidectomy or both was reported to have reduced nocturnal enuresis in up to 76% of patients [42]. In a recent study, enuresis has not only been linked to SDB in children with SCA but the severity of SDB has been observed to have a strong correlation with frequency of nocturnal enuresis [6]. Notably, SDB is a common sleep disorder comprising a spectrum from snoring to obstructive sleep apnea syndrome (OSAS) which may worsen nocturnal enuresis through disrupted sleep and neurologic dysregulation [9]. While the finding of a study suggests that SDB is more prevalent in children with SCA than in the general population [43], overwhelming evidence also shows that a significant relationship exists between SDB and nocturnal enuresis among non-SCA children [44–46].

Furthermore, the role of low levels of some vitamins in the etiopathogenesis of nocturnal enuresis has been highlighted in recent studies. For instance, enuretic children were observed to have lower serum vitamin B12 and folate levels than their non-enuretic counterparts [47, 48]. The reduced vitamin levels are believed to be associated with slow cortical maturation which has been linked to enuresis. Interestingly, significantly lower or deficient vitamin B12 levels have equally been reported in children with SCA compared to non-SCA controls [49, 50]. In addition, increased risk of nocturnal enuresis has been observed in vitamin D-deficient children as this vitamin deficiency directly correlated with severity of enuresis [51]. In a recent systematic review, the prevalence of vitamin D deficiency was reported to vary from 56.4% to 96.4% in children with SCA [52]. This link between vitamin D deficiency and nocturnal enuresis can be explained partly by its influence on SDB and nocturnal polyuria. Reports indicate that low level of serum 25 (OH) D was associated with increased risk of developing OSAS [53], as well as primary snoring [54]. The association of low vitamin levels with OSAS is reportedly mediated through promotion of adenotonsillar hypertrophy, chronic rhinitis and/or myopathy of airway muscle [55]. Better still, low vitamin D levels may result in nocturnal enuresis through obstructive sleep apnea, sleep fragmentation and nocturnal polyuria, which

Another etiologic consideration for nocturnal enuresis seen in SCA is its association with some involuntary movements such as periodic limb movement syndrome and restless leg syndrome. The prevalence of periodic limb movement syndrome in SCA has been documented as 20.5–29% [56–58], which was significantly higher than the rates of 1.2–8% reported for non-SCA children [59, 60]. Similarly, a prevalence rate of 11.1% has been observed for children with restless leg syndrome [56]. Notably, both involuntary movements are associated with sleep disruption [60]. Given that enuretic children have higher incidence of periodic limb movement and sleep fragmentation [59] and the higher rate of periodic limb movement syndrome associated nocturnal enuresis and sleep disruption in SCA patients, it is therefore not surprising to observe a high prevalence rate of nocturnal enuresis in them. To underscore the nexus between these aforementioned etiologic factors (low serum vitamin D level and restless leg syndrome), it has been observed that Vitamin D supplementation also improved the severity of this involuntary movement [61].

In summary, the etiopathogenic mechanisms involved in nocturnal enuresis among SCA and non-SCA children are multifactorial and not mutually exclusive, and they include hyposthenuria-related nocturnal polyuria, decreased bladder capacity or nocturnal bladder over-activity, high sleep arousal thresholds and SDB [9] (**Figure 1**).

small bladder contraction and reduced urethral contraction [40].

**16**

*The proposed etiopathogenic mechanisms for nocturnal enuresis. SDB, sleep-disordered breathing; nocturnal polyuria, induced by hyposthenuria. Concept and design: by SNU (one of the authors).*

### **4. Conclusion**

Although nocturnal enuresis appears more prevalent in children with SCA than in their non-SCA counterparts, the exact etiopathogenesis of enuresis is not completely understood. In fact, the suggested mechanisms for nocturnal enuresis in SCA children are also applicable to their non-SCA counterparts. Moreover, the multiregional variations in prevalence rates may be due to differences in definition criteria and study methods. Male predominance in enuretic children has been somewhat established, but there is no unanimity yet on the influence of socioeconomic status on prevalence rates. Perhaps, adopting standardized definitions and study methods may in future minimize the disparities in the reported prevalence rates. More importantly, further research is still required to establish the precise etiopathogenesis of nocturnal enuresis in children with SCA.

#### **Acknowledgements**

The authors acknowledge the invaluable information obtained from the articles Uwaezuoke et al. [62] and Wolf et al. [9].

#### **Disclosure**

The authors declare that there are no conflicts of interest.

### **Author details**

Samuel N. Uwaezuoke1,2\*, Chizoma I. Eneh3 , Osita U. Ezenwosu1,2 and Ikenna K. Ndu3

1 College of Medicine, University of Nigeria, Enugu, Nigeria

2 University of Nigeria Teaching Hospital, Enugu, Nigeria

3 Enugu State University of Science and Technology Teaching Hospital, Enugu, Nigeria

\*Address all correspondence to: samuel.uwaezuoke@unn.edu.ng

© 2018 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/ by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

**19**

*Nocturnal Enuresis in Children with Sickle Cell Anemia DOI: http://dx.doi.org/10.5772/intechopen.81578*

> Task Force on DSM-IV. Diagnostic and Statistical Manual of Mental Disorders: DSM-IV-TR. Washington, DC, USA: American Psychiatric Association; 2000

Ogunmekan AO, Okoye JU. Enuresis in sickle cell disease: Prevalence studies. Journal of Tropical Pediatrics. 1989;**35**(1):24-26. DOI: 10.1093/

[12] Mabiala Babela JR, Loumingou R, Pemba-Loufoua A, Nzingoula S, Senga P. Enuresis in children with sickle cell disease. Archives de Pédiatrie.

[13] Barakat LP, Smith-Whitley K, Schulman S, et al. Nocturnal enuresis in pediatric sickle cell disease. Journal of Developmental and Behavioral Pediatrics. 2001;**22**(5):300-305

[14] Eneh CI, Okafor HU, Ikefuna AN, Uwaezuoke SN. Nocturnal enuresis:

[15] Ogunrinde GO, Zubair RO, Mado SM, Umar LW. Prevalence of nocturnal enuresis in children with homozygous sickle-cell disease in Zaria. Nigerian Journal of Paediatrics. 2007;**34**:31-35

[16] Hansakunachai T, Raungdaraganon N, Udomsubpayakul U, Sombunthan T, Kotchabhakdi N. Epidemiology of enuresis among school-aged children in Thailand. Journal of Developmental and Behavioral Pediatrics. 2005;**26**:365-360

[17] Chiozza ML, Bernadi L, Cainone P, et al. An Italian epidemiological multicenter study of nocturnal enuresis. British Journal of Urology.

Prevalence and risk factors among school-aged children with sickle-cell anaemia in a south-east Nigerian city. Italian Journal of Pediatrics. 2015;**41**:66. DOI: 10.1186/

s13052-015-0176-9

1988;**81**:86-89

[11] Akinyanju O, Agbato O,

tropej/35.1.24

2004;**11**(10):1168-1172

[1] Robert I, de Montalembert M. Sickle cell disease as a paradigm of immigration haematology: New challenges for hematologists in Europe. Haematologica. 2007;**92**:865-875

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Blood Reviews. 2005;**19**:99-110

Medicine. 1979;**90**:310-316

Medicine. 1990;**150**:501-504

10.1016/j.juro.2012.02.021

innovation in the management of sickle cell disease: The road to understanding the sickle cell disease clinical phenotype.

[3] Da Fonzo RA, Taufield PA, Black H, et al. Impaired renal tubular secretion in sickle cell disease. Annals of Internal

[4] Allon M. Renal abnormalities in sickle cell disease. Archives of Internal

[6] Lehmann GC, Bell TR, Kirkham FJ, et al. Enuresis associated with sleep disordered breathing in children with sickle cell anemia. The Journal of Urology. 2012;**188**:1572-1576. DOI:

[7] Readett DR, Morris JR, Serjeant GR. Nocturnal enuresis in sickle cell hemoglobinopathy. Archives of Disease

[8] Readett DR, Morris JS, Serjeant GR. Determinants of nocturnal enuresis in homozygous sickle cell disease. Archives of Disease in Childhood.

[9] Wolf RB, Kassim AA, Goodpaster RL, DeBaun MR. Nocturnal enuresis in sickle cell disease. Expert Review of Hematology. 2014;**7**:245-254. DOI: 10.1586/17474086.2014.892412

[10] American Psychiatric Association. American Psychiatric Association,

in Childhood. 1990;**65**:290-293

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[5] Noll JB, Newman AJ, Gross S. Enuresis and nocturia in sickle cell disease. The Journal of Pediatrics. 1967;**70**:965-967

*Nocturnal Enuresis in Children with Sickle Cell Anemia DOI: http://dx.doi.org/10.5772/intechopen.81578*

## **References**

*Contemporary Pediatric Hematology and Oncology*

**18**

**Author details**

Ikenna K. Ndu3

Nigeria

provided the original work is properly cited.

Samuel N. Uwaezuoke1,2\*, Chizoma I. Eneh3

1 College of Medicine, University of Nigeria, Enugu, Nigeria

\*Address all correspondence to: samuel.uwaezuoke@unn.edu.ng

2 University of Nigeria Teaching Hospital, Enugu, Nigeria

© 2018 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/ by/3.0), which permits unrestricted use, distribution, and reproduction in any medium,

3 Enugu State University of Science and Technology Teaching Hospital, Enugu,

, Osita U. Ezenwosu1,2 and

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[12] Mabiala Babela JR, Loumingou R, Pemba-Loufoua A, Nzingoula S, Senga P. Enuresis in children with sickle cell disease. Archives de Pédiatrie. 2004;**11**(10):1168-1172

[13] Barakat LP, Smith-Whitley K, Schulman S, et al. Nocturnal enuresis in pediatric sickle cell disease. Journal of Developmental and Behavioral Pediatrics. 2001;**22**(5):300-305

[14] Eneh CI, Okafor HU, Ikefuna AN, Uwaezuoke SN. Nocturnal enuresis: Prevalence and risk factors among school-aged children with sickle-cell anaemia in a south-east Nigerian city. Italian Journal of Pediatrics. 2015;**41**:66. DOI: 10.1186/ s13052-015-0176-9

[15] Ogunrinde GO, Zubair RO, Mado SM, Umar LW. Prevalence of nocturnal enuresis in children with homozygous sickle-cell disease in Zaria. Nigerian Journal of Paediatrics. 2007;**34**:31-35

[16] Hansakunachai T, Raungdaraganon N, Udomsubpayakul U, Sombunthan T, Kotchabhakdi N. Epidemiology of enuresis among school-aged children in Thailand. Journal of Developmental and Behavioral Pediatrics. 2005;**26**:365-360

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[22] Jordan SS, Hilker KA, Stoppelbein L, et al. Nocturnal enuresis and psychosocial problems in pediatric sickle cell disease and sibling controls. Journal of Developmental and Behavioral Pediatrics. 2005;**26**(6):404-411

[23] Figueroa TE, Benaim E, Griggs ST, Hvizdala EV. Enuresis in sickle cell disease. The Journal of Urology. 1995;**153**(6):1987-1989

[24] Field JJ, Austin PF, An P, Yan Y, DeBaun MR. Enuresis is a common and persistent problem among children and young adults with sickle cell anemia. Urology. 2008;**72**(1):81-84. DOI: 10.1016/j.urology.2008.02.006

[25] Ekinci O, Celik T, Ünal Ş, Oktay G, Toros F. Nocturnal enuresis in sickle cell disease and thalassemia major: Associated factors in a clinical sample. International Journal of Hematology. 2013;**98**(4):430-436. DOI: 10.1007/ s12185.013.1422.9

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*Contemporary Pediatric Hematology and Oncology*

[26] Unalacak M, Sögüt A, Aktunç E, Demircan N, Altin R. Enuresis nocturna: Prevalence and risk factors among school-aged children in

Northwest Turkey. European Journal of

[28] Chandra M. Nocturnal enuresis in children. Current Opinion in Pediatrics.

[29] Kawauchi A, Tanaka Y, Naito Y, Yamao Y, Ukimura O, Yoneda K, et al. Bladder capacity at the time of enuresis.

[30] Reid CD. Renals. In: Bertnam L, Reid CD, Charache C, Lubin B, editors. Management and Therapy of Sickle Cell Disease. 3rd ed. Maryland: NIH Publication; 1995. pp. 95-100

[31] Zadeii G, Lohr JW. Renal papillary necrosis in a patient with sickle cell trait. Journal of the American Society of

Nephrology. 1997;**8**:1034-1039

1995. pp. 1-5

pp. 311-326

2007;**2**:45-50

[32] Shayman JA, editor. Water. In: Lippincott's Pathophysiology Series: Renal Pathophysiology. 2nd ed. Philadelphia: J.B Lippincott Company;

[33] Embury SH, Hebbel RP, Steinberg MH, Mohandas N. Pathogenesis of vaso-occlusion. In: Embury SH, Hebbel RP, Steinberg MH, editors. Sickle Cell Disease. Basic Principles and Clinical Practice. 1st ed.

Philadelphia: Lippincott-raven; 1995.

abnormalities in children with sickle cell anemia. Port Harcourt Medical Journal.

[34] Ugwu RO, Eke FU. Urinary

Urology. 2003;**61**:1061-1068

General Medicine. 2004;**1**:21-25

[27] Cher TW, Lia GJ, Hsu KH. Prevalence of nocturnal enuresis and associated familial factors in primary school-aged children in Taiwan. The Journal of Urology. 2002;**168**:1142-1146

1998;**10**:167-173

[18] Golding J, Tassier G. Soiling and wetting. In: Butler NR, Golding J, editors. From Birth to Five. Oxford: Pergamon Press; 1986. pp. 64-79

[19] Garfinkel BO. The elimination disorders. In: Garfinkel BO, Carlson GA, Weller EB, editors. Psychiatric Disorders in Childhood and Adolescents. 2nd ed. Philadelphia: WB Saunders (Publ); 2000.

[20] Obi JO. Enuresis in Nigerian children as seen in Benin City. African Journal of Psychiatry. 1977;**1**:65-68

[21] Portocarrero ML, Portocarrero ML, Sobral MM, et al. Prevalence of enuresis and daytime urinary incontinence in children and adolescents with sickle cell disease. The Journal of Urology. 2012;**187**(3):1037-1040. DOI: 10.1016/j.

[22] Jordan SS, Hilker KA, Stoppelbein L, et al. Nocturnal enuresis and psychosocial problems in pediatric sickle cell disease and sibling controls. Journal of Developmental

and Behavioral Pediatrics. 2005;**26**(6):404-411

1995;**153**(6):1987-1989

[23] Figueroa TE, Benaim E, Griggs ST, Hvizdala EV. Enuresis in sickle cell disease. The Journal of Urology.

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Section 3

Inherited Bone Marrow

Failure Syndromes

Section 3
