**3. Results**

A total of 20 periodontally healthy children accepted to participate in this study; 8 were males and 12 were females. The average age was 10.52 ± 2.78 years (range, 6–16 years). The patients' general characteristics are as shown in **Table 2**.

#### **3.1 Correlation between salivary LPS-Pg and Ig-E levels**

Analysis of correlation between salivary LPS-Pg and Ig-E levels showed a strong correlation (r2 = 0.695; *p* < 0.001, n = 20), and there was a positive correlation as shown in **Figure 1**.

### **3.2 Correlation between salivary LPS-Pg and Ig-G4 levels**

Analysis of correlation between salivary LPS-Pg and Ig-G4 levels showed a very strong correlation (r2 = 0.796; *p* < 0.001, n = 20), and there was a positive correlation as shown in **Figure 2**.

#### **3.3 Results of univariate logistic regression analysis**

As shown in **Table 3**, univariate logistic regression analysis uses gender, age, height, weight, BMI, family size, insurance status, number of colony-forming unit (CFU) of Pg, level of salivary LPS-Pg, level of circulatory Ig-G4 as the independent variables, and circulatory Ig-E as the dependent variable. Data revealed that number of colony-forming units (CFU) of Pg, level of salivary LPS-Pg, and level of circulatory Ig-G4 were associated with circulatory Ig-E (*p* < 0.05).

#### *Gingival Disease - A Professional Approach for Treatment and Prevention*


*BMI, body mass index; CFU-Pg, number of colony-forming units of Porphyromonas gingivalis; LPS-Pg, level of salivary lipopolysaccharide of Porphyromonas gingivalis; Ig-G4, level of circulatory Ig-G4.*

#### **Table 2.**

*Demographic information of the study participants.*

#### **Figure 1.**

*Correlation between salivary lipopolysaccharide of Porphyromonas gingivalis concentration and circulatory Immunoglobulin-E level. Strong correlation between salivary lipopolysaccharide and circulatory Ig-E had been observed (r2 = 0.695; p < 0.001, n = 20).*

**37**

**Table 3.**

**Figure 2.**

*observed (r2*

**3.4 Results of multivariable regression analysis**

*salivary lipopolysaccharide of Porphyromonas gingivalis; Ig-G4, level of circulatory Ig-G4.*

*The results of univariate linear regression analysis for Ig-E as dependent variable.*

risk factor for circulatory Ig-E (**Table 4**).

Multivariable regression analysis was performed using indexes which were demonstrated to be related to circulatory Ig-E in univariate analysis. The results showed that only level of salivary LPS-Pg and level of circulatory Ig-G4 remained a

*Correlation between Salivary Lipopolysaccharide of Porphyromonas gingivalis with Circulatory…*

*Correlation between salivary lipopolysaccharide of Porphyromonas gingivalis concentration and circulatory Immunoglobulin-G4 level. Very strong correlation between salivary lipopolysaccharide and Ig-G4 had been* 

**Variable β S.E** *p* **value Min. Max.** Gender −0.130 7.953 0.418 −24.746 11.234 Age 0.360 2.904 0.273 −3.179 9.958 Height −2.120 2.450 0.165 −9.248 1.838 Weight 1.814 4.858 0.250 −5.019 16.962 BMI −0.950 9.665 0.212 −34.843 8.886 Family size −0.181 2.501 0.202 −9.100 2.216 Insurance 0.155 6.252 0.247 −6.403 21.884 CFU-Pg −0.082 0.466 0.595 −1.311 0.798 LPS-Pg −0.017 2.271 0.951 −5.280 4.993 Ig-G4 0.999 0.758 0.004 1.163 4.595 *BMI, body mass index; CFU-Pg, number of colony-forming units of Porphyromonas gingivalis; LPS-Pg, level of* 

*DOI: http://dx.doi.org/10.5772/intechopen.81113*

 *= 0.796; p < 0.001, n = 20).*

*Correlation between Salivary Lipopolysaccharide of Porphyromonas gingivalis with Circulatory… DOI: http://dx.doi.org/10.5772/intechopen.81113*

#### **Figure 2.**

*Gingival Disease - A Professional Approach for Treatment and Prevention*

Male 8 Female 12

Public 17 Private 2 None 1

*Demographic information of the study participants.*

Gender (n)

BMI (kg/m2

CFU-Pg (×105

**Table 2.**

Insurance status (n)

**Variable Mean (SD) Minimum Maximum**

) 18.24 (1.92) 15.27 21.82

CFU/ml) 9.93 (8.34) 1.08 29.45

Age (years) 10.52 (2.78) 6 16 Height (cm) 140.28 (14.99) 108.50 162.00 Weight (kg) 36.12 (7.96) 23.00 51.00

Family size (n) 4.70 (1.38) 3 8

LPS-Pg (μg/ml) 7.21 (3.06) 1.96 10.76 Ig-E (pg/ml) 114.44 (26.20) 77.69 141.41 Ig-G4 (ng/ml) 31.02 (9.09) 15.96 40.96 *BMI, body mass index; CFU-Pg, number of colony-forming units of Porphyromonas gingivalis; LPS-Pg, level of* 

*salivary lipopolysaccharide of Porphyromonas gingivalis; Ig-G4, level of circulatory Ig-G4.*

**36**

**Figure 1.**

*observed (r2*

 *= 0.695; p < 0.001, n = 20).*

*Correlation between salivary lipopolysaccharide of Porphyromonas gingivalis concentration and circulatory Immunoglobulin-E level. Strong correlation between salivary lipopolysaccharide and circulatory Ig-E had been* 

*Correlation between salivary lipopolysaccharide of Porphyromonas gingivalis concentration and circulatory Immunoglobulin-G4 level. Very strong correlation between salivary lipopolysaccharide and Ig-G4 had been observed (r2 = 0.796; p < 0.001, n = 20).*


*BMI, body mass index; CFU-Pg, number of colony-forming units of Porphyromonas gingivalis; LPS-Pg, level of salivary lipopolysaccharide of Porphyromonas gingivalis; Ig-G4, level of circulatory Ig-G4.*

#### **Table 3.**

*The results of univariate linear regression analysis for Ig-E as dependent variable.*

#### **3.4 Results of multivariable regression analysis**

Multivariable regression analysis was performed using indexes which were demonstrated to be related to circulatory Ig-E in univariate analysis. The results showed that only level of salivary LPS-Pg and level of circulatory Ig-G4 remained a risk factor for circulatory Ig-E (**Table 4**).


*CFU-Pg, number of colony-forming units of Porphyromonas gingivalis; LPS-Pg, level of salivary lipopolysaccharide of Porphyromonas gingivalis; Ig-G4, level of circulatory Ig-G4.*

**Table 4.**

*The results of multivariable logistic regression analysis for Ig-E as dependent variable.*
