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## Meet the editors

Dr. Marko S. Sabovljević is a biologist with a master's degree in Biology from the University of Belgrade, Serbia (2003) and a doctoral degree from the University of Bonn, Germany (2006). His research is dedicated to various aspects of bryophyte biology, and he has published his research in nearly 500 peer-reviewed articles. He has also authored or coauthored 11 book chapters, 189 communications, and several university and

academic textbooks and monographs. He is a peer reviewer, coeditor and editorial board member for many international journals and is Editor-in-Chief of Botanica Serbica. Currently, Dr. Sabovljević is a fulltime professor of Ecology, Biogeography and Environmental Sciences in the Department of Plant Ecology and Phytogeography, Institute of Botany and Botanical Garden, Faculty of Biology, University of Belgrade, Serbia.

Dr. Aneta D. Sabovljević is a biologist with a master's degree in Biology from the University of Belgrade, Serbia (2003) and a doctoral degree from the University of Belgrade, Serbia (2007). She has a rich national and international research career, and is interested in studying bryophytes both in the lab and in the field. She has mostly experimental and analytical approaches to bryophyte biology. Dr. Sabovljević has published her research

results in more than 100 peer-reviewed articles. She is a peer reviewer for many national and international scientific journals. Currently, she is Associate Professor of Physiology and Molecular Biology of Plants in the Department of Plant Physiology, Institute of Botany and Botanical Garden, Faculty of Biology, University of Belgrade, Serbia.

### Contents


## Preface

Bryophytes are a group of plants with around 22,500 species worldwide. It is a diverse group that includes many subgroups that are phylogenetically rather different from one another, although they share a common and unique life cycle. There are marked differences between organisms in this group, including various life strategies, life forms, survival and dispersal possibilities, chemical constituents, biochemical patterns, physiological mechanisms and ecological responses. Indeed, there is such an immense diversity that we are still far from having satisfactory knowledge of bryophytes. Even if we study more classical disciplines such as biogeography, there is still much we don't know about bryophytes and their potential applications.

In this book, we offer new views, novel data and various approaches to bryophyte sciences. Chapters cover distribution changes of *Sphagna* moss, particularly *Cuspidata*, in the East European Plain and Eastern Fennoscandia, bryophyte diversity within the forests of the Southern Urals (Russia), the antioxidant potential of bryophytes, and the role of ohioensins, which are chemical constituents of some bryophyte species, in curing disease.

The editors acknowledge the contributing authors as well as the entire IntechOpen publishing team for all their attention and support, without which this book would not be possible.

#### **Dr. Marko S. Sabovljević**

Full Professor in Ecology, Biogeography and Environmental Sciences, Faculty of Biology, Department of Plant Ecology and Phytogeography, Institute of Botany and Botanical Garden, University of Belgrade, Serbia

#### **Dr. Aneta D. Sabovljević**

Associate Professor in Physiology and Molecular Biology of Plants, Faculty of Biology, Department of Plant Physiology, Institute of Botany and Botanical Garden, University of Belgrade, Serbia

#### **Chapter 1**

## Introductory Chapter: Bryophytes 2020

*Marko S. Sabovljević and Aneta D. Sabovljević*

#### **1. Bryophytes**

Bryophytes, amphibians within the plant kingdom, were among the first plants that lived in the water and settled in terrestrial environment. They faced such a harsh and bare environment they needed to cope with; but on the other hand, these facts gave opportunities to diversify so much. Nowadays, they count between 18,000 and 23,000 extant species [1]. However, one can estimate many undescribed species that appear with classical and molecular approaches, and every year many new species for science are reported, even from Europe [2] that is bryologically among the best investigated world areas. Classification of such a huge diversity as bryophytes is the matter of discussions leading to treating them as one division or alternatively three or more within the common subkingdom of plants named Bryobiotina. Irrelevant of classification level, the three widely accepted group can be considered within bryophytes: mosses (11,000–13,000 species), liverworts (7000–9000 species), and hornworts (200–250 species).

During the decades of accumulation on the knowledge on bryophytes, bryophyte science developed and changes our views on the three group relationships among themselves and with other plants and algae. One of the latest views is that mosses and liverworts can be considered as Setaphyta while hornworts (Anthocerotophyta) seems to be near vascular plants [3].

Nevertheless, these groups share common ancestor, as well as a number of biological and ecological traits. Even though some lignin-like compounds are discovered to be present in some species, they do not produce lignin which preclude them to develop into huge forms due to the absence of mechanical body support. They are all rather small plants, even some species can reach few decimeters in height. The life cycle of bryophytes is dominated by haploid gametophytes, while diploid sporophyte has even shorter appearance during the sexual reproduction.

The strong cuticles are absent unabling them to keep body water balance. This means they are water dependent from the water balance in the immediate environment, i.e., poikilohydric. Thus, many of bryophytes can go to anabiosis which means drying out during dry period and once the wet period is back they can quickly resume their metabolism with no damage. This is why many species belong also to the group of unrelated vegetation representatives called resurrection plants.

The lack of cuticles, whole body collecting water from the atmosphere, and the absence of rootlike structure for soaking (rhizoids have mainly anchoring function) are some of the characteristics which makes them good bioindicator species quickly reacting to small changes in proximate environment. So, bryophytes possess a huge potential in specific sensitivity also due to their relations to specific microhabitats and proximate ecological conditions, and they greatly exceed the sensitivity of vascular plants to pollutants or in general environmental changes.

Bryophytes inhabit almost all ecosystems on the Earth. There are no representatives in the seas, but there are representatives in the brackish waters or moderate salt environment [4]. Though, over the times we learn that they have significant roles within the communities they live in. A range of significant ecological functions of bryophytes are huge and vary from biomes to biomes, but the general ones include water retention (acting like a huge sponge; they play a significant role in water balancing in the ecosystems), carbon sequestration (i.e., carbon locking by peatland dominated by peat mosses), or biotic interaction with other organisms (e.g., providing shelters, acting as a seed bed, or representing habitat *per se* for many other organisms).

The geographical ranges of bryophytes are wider than those of vascular plants [5]. This is due to the long-distance dispersal of small spores, huge survival rate or diaspores during transportation, and settling specific microhabitats over the huge areas. In general, we can say they are ubiquitous, since species can be found in dry desert to the underwater deep in freshwater lakes and from the sea level till the top of the highest mountains surviving even under the long laying ice. Their nutrient supply is over the whole body surface coming from precipitates. Drying out, i.e., suspending physiological activities, versus rewetting, i.e., establishing back normal life function, can occur on a daily basis (e.g., *Grimmia* and *Schistidium* that live on exposed rocks), or they can survive longer periods of inactivity upon dehydration (many members of Pottiaceae).

Most of the bryophyte species are rather less competitive to resources in the environment than vascular plants. Thus, they have a wide range of distinctive feature to survive including, beside the abovementioned, life forms and life strategies. Also dispersal and propagation can be through various vectors both biotic (e.g., birds, snails, mammals) and abiotic (e.g., wind, watercourses), and apart from spores that are produced sexually, diaspores can be produced on rhizoids (e.g., *Bryum*), stem tips (*Aulacomnium*), or on the leaves (*Pohlia*, *Orthotrichum*, etc*.*) for vegetative spread. Even parts of the whole bodies can serve for this purpose. Such an efficient possibility for wide dispersion and long viability of spores and diaspores enables them to rich long distances, and this is why the endemism is rather low compared to vascular plants. Some authors stated that 10% among European bryophytes express the endemic characters compared to 28% of tracheophytes. On the other hand, the discontinuous ranges and disjunctions are very high among bryophytes [5] as a consequence of very efficient spread and the microhabitat importance for new population establishment.

Among the interesting features of bryophytes, being an ecological indicator should not be passed by (e.g., [6–8]). Many species occur on specific pH of the substrate, or indicate by appearance air quality. Additionally, some species are so well adapted to substrate and nutrients coming from dissolved substrate by precipitation that they can indicate the presence of salt (e.g., *Entosthodon hungaricus*) [9] or minerals (Pb, *Ditrichum plumbicola*; Cu, *Scopelophila cataractae*) [10] and are strict to such a region.

Fast-spreading protonemal growth in a short suitable period can stabilize the soil surface, preventing erosion. Also, they are pioneer colonizers and stabilizers of bare surface, enriching the ecosystems and producing a suitable habitats for further settlers in successional phases of ecosystem changes/development. Spreading colonies on rocks, they initiate soil establishment and participate in protosoil production cohabiting with cyanobacteria, playing an important role in nitrogen fixation, i.e., enabling colonization for other plants and organisms.

Bryophytes have no huge commercial values, at present. These values come from a huge number of both biotic and abiotic interactions as well as their peculiarities (e.g., [6, 11, 12]). However, they have huge applicative potential, which is lately noticed but still neglected compared to vascular plants.

#### *Introductory Chapter: Bryophytes 2020 DOI: http://dx.doi.org/10.5772/intechopen.91420*

Chemical constituents of bryophytes attract lately very much attention since many new to science and rare or modified known compounds are discovered in different species [13–16]. Also, bioactivities of extracts or target compounds are promising for bio-industrial products such as biopharmaceutical, biopesticides, biorapelents, or cures. Since all these products are environmentally friendly, new biotechnological processes with bryophytes are needed to be established to get to the point when wide use can be done. The treat to some modern diseases like AIDS and different cancer types and even new antibiotics are possible to develop from bryophytes [17–19]. Huge potential of bryophytes are seen by cosmetic industry as well. The problems remain the small biomass in nature for such a project, hard identification, monoculturing, cohabitation, and interfusion with many other organisms. There are steps forward to establish bryo-reactors with selected species to overcome these problems, but still clean start material is needed to do so. Therefore, the axenic and in vitro establishment of target taxa is necessary. This is not an easy task, having in mind that many species are hardly available and not in a proper developmental stage, and also due to lack of cuticles, one-cell thalli layers that unable or hardening surface sterilization without killing target material as well. Additional problem can be endophytic cohabitants.

Though, there are many advantages in bryophytes. For example, easy gene targeting and high rate of homologous recombination are the main pathways for transforming DNA to incorporate in moss genome [5, 20]. This is surely true for the model moss whose genome is completely sequenced, namely, *Physcomitrella patens.* It is widely studied and exhibits high frequencies of gene targeting. DNA constructs with sequences homologous to genomic loci can transform moss rather easy. The outcome then is the organism with targeted gene replacement resulting from homologous recombination although untargeted integration at nonhomologous sites can also occur, but at a significantly lower frequency which can be easily eliminated.

Since, these organisms are rather microhabitat dependent and sensitive to environmental changes, large-scale harvesting and impulsive climate change can cause both diversity and biomass loss not only damaging bryophytes *per se* but the global ecosystem as well. Thus, protection and conservation for the bryophytes are urgently needed in a quick-changing world [21]. Many governments and conservationist have already done a lot in legislative, giving priority to highly threatened species, i.e., applying passive measures for the well-being of mosses, liverworts, and hornworts or habitats they live in. However, it seems these are not enough, and the decrease in populations, even species loss, is taking part. Therefore active conservation measures are needed: species propagation, species reintroduction, habitat management, and constant monitoring [22–27]. The emerging field of conservation biology, namely, conservation physiology, is therefore needed to learn in experimental both laboratory and field conditions, those what is essential on species biology prior to decision which measures will be applied for good species conservation and loss prevention apart from legal measure.

And again, in vitro establishment, studies, and propagation arise as problem solutions in maintaining ex situ collections and preparing material for release to the wild [25, 27]. The reviving of material stored in herbarium is sometimes possible [26], but in most cases good green material is needed which mostly is not the case.

Many problems in different fields of bryophyte sciences remain to be solved, and many phenomena remain to be uncovered, although over the past century many knowledge on bryophyte biology were accumulated. However, in 2020 we still need both to spread among known fact searching for overlooked and to go deeper beyond the point that has been reached up to date.

*Bryophytes*

#### **Author details**

Marko S. Sabovljević\* and Aneta D. Sabovljević Faculty of Biology, Institute of Botany and Botanical Garden, University of Belgrade, Belgrade, Serbia

\*Address all correspondence to: marko@bio.bg.ac.rs

© 2020 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/ by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

*Introductory Chapter: Bryophytes 2020 DOI: http://dx.doi.org/10.5772/intechopen.91420*

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#### **Chapter 2**

### Bryophyte Diversity in the Forests of the Southern Urals

*Elvira Baisheva, Pavel Shirokikh and Vasiliy Martynenko*

#### **Abstract**

Mountain forest monitoring is closely related to the survey of bryophyte species since it is there that these organisms are common and show very specialized ecological niches. This work is aimed to show how bryophyte richness and taxonomic and ecological categories differ in the various types of indigenous forests in the Southern Ural Mountains. The distribution of bryophytes in mountain forests of the Southern Urals was examined at about 1700 sample plots. Frequency and abundance patterns suggested that species richness, taxonomic distribution, and substrate group distribution are mostly determined by the forest type. According of bryological data, the forest associations characterized by high diversity and concentration of rare species were identified. This is mainly tall herb spruce-fir and mixed forests. The proportion of rare species in these forests is about 9%, including a significant number relicts both of European and Asian origins. The sites of these forests are most valuable for nature conservation and should be protected.

**Keywords:** bryophytes, biodiversity, forest vegetation, the Southern Urals, syntaxonomy, nature protection

#### **1. Introduction**

The Southern Urals is the southern part of Ural Mountains that is also called the Urals, or Ural, a mountain range in west-central Russia which borders with East European Plain to the west and West Siberia to the east. The vegetation in the Southern Urals is characterized by high diversity. It depends on a number of regional factors: unique geographic position between Europe and Asia; mountains being an important climatic boundary, causing significant differences in vegetation of the western and eastern slopes; and the absence of Pleistocene glaciation, which allowed to preserve the ancient elements of the vegetation [1].

Currently, the forests cover about 80% of the Southern Urals [2]. The modern altitudinal forest belt and floristic composition of current Urals forests were formed over the last 4500 years during the subboreal and sub-Atlantic periods of the Holocene. At the same time, the Ural ridge became a natural physical and geographical boundary for the ranges of many nemoral species due to the increasing of climate continentality from west to east [3]. The Ural ridge is a natural barrier to the path of moist and warm Atlantic air masses. For this reason, the climate on the western Ural's slope is humid and warm; it is more favorable for the deciduous forests. On the eastern slope, the climate is more continental, which led to the dominance of hemiboreal pine-birch and larch forests of West Siberian type and steppe communities. In the middle of the central elevated part of the Southern Urals, the

spruce-fir and mixed broad-leaved and dark coniferous forests are widespread. Thus, the Southern Urals is a contact area of three types of forest vegetation, that is, (1) East European broad-leaved deciduous forests, (2) mountain taiga, and (3) hemiboreal Siberian pine, larch, and birch forests. The presence of the species from three complexes, nemoral, boreal, and hemiboreal, significantly increases species richness of Ural forests [3–5].

In comparison with North European forests, where agriculture started to reduce in the forest area 3000–5000 years ago [6], the history of forest exploitation in the Southern Urals is relatively short: the large-scale felling has been carried out here for 300 years. Nevertheless, intensive cutting of the mountain forests in this area has brought about a highly mosaic structure of vegetation represented by various types of forests at different stages of regeneration and age succession [7]. Also, heavily exploitation of mountain forests causes a major part of their biodiversity value to be lost.

One of the important challenges of forest ecology is reconstruction of the picture of the original species composition and structure of the forests (in relation to overall biodiversity, space, age, thickness of trees, composition of understory vegetation, etc.). The preserved parts of indigenous forests represent an important base for knowledge about the original diversity and structure of forests of a particular region. The identification of the most vulnerable and valuable forest sites in terms of the conservation of forest biodiversity is an important conservation challenge which attracts the attention of specialists from different fields. There are several approaches to the selection of these areas, which differ by their significance, sizes, and level of conservation. In the vegetation science, there are many categories of "etalon" forest communities: forests of high conservation value, intact forest landscapes (IFA), biologically valuable forests (IVF), etc. [8]. Despite the differences in the interpretation of the terms "indigenous," "old-growth," or "intact" forests, most of the researches keep in mind forest communities that have developed over a long period of time (comparable to the maximum biological age of tree species or exceeding this age) without or with minimal human impact [9]. In our research, we identify these forests as "relatively indigenous," or "indigenous," keeping in mind that at least some of them were established in the sites that were more or less human-modified in the past. It should be noted that many types of indigenous forest communities of the Southern Urals have been preserved only by small fragments in areas difficult to access for logging or in specially protected natural areas [10, 11].

Bryophytes represent a significant component of overall forest diversity and play important roles in ecosystem processes and functions [12], but in European Russia the bryophyte diversity in particular types of forest is not well studied [13–15].

In the last three decades, we are specifically interested in the revealing of bryophyte diversity in the forests of the Southern Urals and the presence of rare species in the different forest types of the region. In particular, we intended to answer the following questions: (1) Do bryophyte diversity vary in relation to environmental variables linked to different forests types? (2) Are rare and endangered species mainly associated with particular forest types? (3) What differences exist between bryophyte diversity in indigenous and secondary forest communities established after felling?

#### **2. Study area and characteristic of investigated forest types**

The research was performed between 1991 and 2017 in the territory of the Republic of Bashkortostan and some adjacent areas of Chelyabinsk Region (Russian Federation). The climate of study area is moderately continental, with relatively

*Bryophyte Diversity in the Forests of the Southern Urals DOI: http://dx.doi.org/10.5772/intechopen.88301*

**Figure 1.**

*Map of study area and the districts of natural zoning of the Southern Urals according to the scheme of A. Muldashev [16].*

warm summer and long cold winter. The average annual temperature is +0.5 to +2.0; the mean temperature in January is −15.5 to −17°C and in July +16.5 to +17.5°C. The mean annual precipitation is 500–700 mm. The frost-free period is 50–90 days; the mean snow depth is 60–75 cm [2].

According to natural zonation of the Republic of Bashkortostan (**Figure 1**), the study area belongs to six districts [16]:


as pine broad-leaved forests, were widely distributed here. Currently, the indigenous vegetation is preserved in small areas, on steep slopes, and in the water-protected zone of the Inzer River. Most of the district's area is covered by secondary birch, aspen, and mixed deciduous forests. Steppe communities occur on steep rocky slopes in the canyons of mountains rivers. The area is sparsely populated.


The main forest trees of the Southern Urals are *Picea obovata* Ledeb., *Abies sibirica* Ledeb., *Pinus sylvestris* L., *Larix sibirica* Ledeb., *Tilia cordata* Mill., *Acer*  *platanoides* L., *Quercus robur* L., *Ulmus glabra* Huds., *Betula pendula* Roth., *Betula pubescens* Ehrh., *Populus tremula* L., and *Alnus incana* (L.) Moench and *Padus avium* Mill. The floodplain forests are formed by *Populus nigra* L., *Populus alba* L., and *Salix* spp.; also paludified floodplain forests with predominance of *Alnus glutinosa* (L.) Gaertn. are occasionally found.

Vegetation classification of the indigenous forests of the Southern Urals consistent with the Braun-Blanquet approach [17] allowed to separate 42 associations which belonged to 10 sub-alliances, 9 alliances, 4 orders, and 4 classes [10]. In total, bryophyte richness was studied in 11 types of forest communities (F1–F11) separated at the level of alliances and sub-alliances. The syntaxonomical position of investigated forest types is given below:

Class CARPINO-FAGETEA SYLVATICAE Jakucs ex Passarge 1968 (syn. Querco-Fagetea Br.-Bl. et Vlieger in Vlieger 1937)—mesic deciduous and mixed coniferousbroadleaved forests of temperate Europe, the Caucasus and Southern Siberia [18].

Order FAGETALIA SYLVATICAE Pawłowski 1928.

**F1**—Alliance Alnion incanae Pawłowski et al. 1928.

**F2**—Alliance Lathyro pisiformis-Quercion roboris Solomeshch et Grigoriev in Willner et al. 2015.

Alliance Aconito lycoctoni-Tilion cordatae Solomeshch et Grigoriev in Willner et al. 2016.

**F3**—Sub-alliance Aconito septentrionalis-Tilienion cordatae Martynenko 2009 prov.

**F4**—Sub-alliance Tilio cordatae-Pinenion sylvestris Martynenko et Shirokikh in Martynenko 2009 prov.

Class ASARO EUROPAEI-ABIETETEA SIBIRICAE Ermakov, Mucina et Zhitlukhina in Willner et al. 2016—cool-temperate coniferous and mixed montane forests with nemoral and hemiboreal floristic elements of the Southern Urals and Southern Siberia [18].

Order ABIETETALIA SIBIRICAE (Ermakov in Ermakov et al. 2000) Ermakov 2006.

**F5**—Alliance Aconito septentrionalis-Piceion obovatae Solomeshch, Grigoriev, Khaziakhmetov et Baisheva in Martynenko et al. 2008.

Class VACCINIO-PICEETEA Br.-Bl. in Br.-Bl. et al. 1939—holarctic coniferous forests on oligotrophic and leached soils in the boreal zone and at high-altitudes of mountains in the nemoral zone of Eurasia [18].

Order PICEETALIA EXCELSAE Pawłowski et al. 1928.

Alliance Piceion excelsae Pawłowski et al. 1928.

**F6**—Sub-alliance Atrageno sibiricae-Piceenion obovatae Zaugolnova et al. 2009.

**F7**—Sub-alliance Eu-Piceenion abietis K.-Lund 1981.

Order PINETALIA SYLVESTRIS Oberd. 1957.

**F8**—Alliance Dicrano-Pinion sylvestris (Libbert 1933) W. Matuszkiewicz 1962. Class BRACHYPODIO PINNATI-BETULETEA PENDULAE Ermakov et al.

1991 hemiboreal pine and birch-pine herb-rich open forests on fertile soils of the Southern Urals and Southern Siberia [18].

Order CHAMAECYTISO RUTHENICI-PINETALIA SYLVESTRIS Solomeshch et Ermakov in Ermakov et al. 2000.

**F9**—Alliance Caragano fruticis-Pinion sylvestris Solomeshch et al. 2002.

**F10**—Alliance Veronico teucrii-Pinion sylvestris Ermakov et Solomeshch in Ermakov et al. 2000.

**F11**—Alliance Trollio europaea-Pinion sylvestris Fedorov in Ermakov et al. 2000.

Short characteristic of the investigated forest types is given in **Table 1**.


**Table 1.**

*Characteristic of the forest types studied (F1–F11) in the Southern Ural Mountains, Russia.*

#### **3. Field sampling**

In about 400 localities, we have laid 1700 sample plots with area of 400 m<sup>2</sup> (usually, not <10 plots for each of forest association). In each plot, the phytosociological relevés, following the Braun-Blanquet method [17], were carried out. All bryophytes growing on various substrates (soil, tree trunks, rotten wood, rock outcrops, etc.) were described.

An estimate of the abundance of forest floor bryophyte species was carried out on the basis of the Braun-Blanquet scale: r, a single species is encountered (the cover is insignificant); +, the species is rare and has a small projective cover up to 1%; 1, the projective cover of species is 1–5%; 2, projective cover is 6–25%; 3, projective cover is 26–50%; 4, projective cover is 51–75%; and 5, projective cover is more than 75%. In synoptic **Table 4**, the following scale of constancy was used: r, the species was encountered in <5% of the relevés; +, in 6–10% of relevés; I, in 11–20% of relevés; II, 21–40% of relevés; III, 41–60% of relevés; IV, in 61–80% of relevés; V, in 81–100% of relevés. Classification of forest vegetation was conducted according to the Braun-Blanquet approach using the TURBOVEG database [19] and program JUICE [20]. Similarity of species diversity between different forest types was calculated using the Jaccard index with program IBIS [21].

Additionally, collections of bryophytes were made outside the sample plots of phytosociological relevés (on forest roads, banks of streams, on bare soil near the roots of fallen trees, etc.). These data were used for compiling of checklist of bryophytes revealed in the forests of the Southern Urals. The specimens are kept in the Herbarium of Ufa, Institute of Biology—Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences (UFA), and partly in Herbarium of the Main Botanical Garden of Russian Academy of Sciences (MHA).

#### **4. The species richness and substrate groups of bryophytes in different types of forest communities**

Forest areas of the Southern Urals have a rich bryophyte flora, presenting 286 species of Bryophyta distributed across 124 genera and 44 families and 58 species of Marchantiophyta from 33 genera and 23 families. This represents 75 and 62% of the moss and liverwort richness of the Republic of Bashkortostan, respectively. High proportions of mountain forest species in the total bryophyte flora of the Bashkortostan stress the great importance of forest protection for preserving biodiversity in the republic.

The predominant families, in terms of the number of species, are Brachytheciaceae (26 species), Dicranaceae (23), Grimmiaceae (20), Amblystegiaceae (20), Sphagnaceae (18), Bryaceae (17), Mniaceae (16), Pottiaceae (14), Polytrichaceae (11), and Pylaisiaceae (12), and the leading genera are *Sphagnum* (18 species), *Dicranum* (18), *Bryum* (15), *Brachythecium* (11), *Pohlia* (10), *Schistidium* (10), *Orthotrichum* (8), *Grimmia* (7), and *Plagiomnium* (7).

Some species richness indicators within studied forest types are given in **Table 2**. The mosses have the leading position in the bryophyte flora of all investigated forests where they held 78–93% of the total species richness within different forest types (**Table 2**). The participation of liverworts is less significant; their richness increases only in the forests with dominance of dark coniferous trees (F5–F7).

The highest species richness in the dark coniferous and mixed tall-herb forests (types F5 and F6) can be explained, mainly, by the relatively high humidity in their habitats, as well as high diversity of epixylic and ground species in these communities. The lowest bryophyte diversity was revealed in the pine, birch, and larch forests (F7, F10, and F11) that developed in the more continental climate conditions of eastern slope of the Southern Urals, and in the oak forests (F2), growing on the western slope but in the forest-steppe sites.

The use of the Jacquard index (**Table 3**) determining the proportion out of the total species list for two forest types which is common to both revealed low


#### **Table 2.**

*Some bryophyte richness indicators of the forest types studied (F1–F11) in the Southern Ural Mountains, Russia.*


#### **Table 3.**

*Jaccard index (similarity matrix) of bryophytes for different forest types in the Southern Ural Mountains, Russia.*

similarity of bryophytes of different forests: the values of the index varied from 0.25 to 0.64. Species compositions of spruce-fir and dark coniferous broad-leaved forests (F5 and F6) are more similar as well as of various pine forests (F8–F10).

These relatively low values of Jaccard index can be explained by the high proportion of species with low constancy (**Table 4**), because, in general, the bryophytes have the scattered distribution within landscapes and vegetation types [22]. In the investigated forests, about 25% of species were revealed from one to three times. The proportion of species that were found less in 10 sample plots was higher in the xero-mesophytic oak, pine, larch, and birch forests growing on the border of forest and steppe (F2, 66%; F10, 66%; F9, 60%). Species with high frequency are few and consist of 5–15% of the bryophyte flora of different forest types.

Bryophytes are well adapted to the forest habitats and can be classified according to the substrate they live on as ground or epigeic (growing on the soil), epiphytic (on the bark of living trees), epixylic (on deadwood), or epilithic (on rock surfaces). Many bryophyte species are able to live on different substrates [23, 24]. It was shown that tree bases are a transition zone between the species growing on the rotten wood (two-thirds of epixylic bryophytes were found there) and epiphytic species growing on upper part of trunks. Also, almost one-third of species of the forest floor were revealed on decaying wood [11].

In all studied forests, xero-mesophytic species *Hypnum pallescens* (Hedw.) P. Beauv., *Sanionia uncinata* (Hedw.) Loeske, *Dicranum montanum* Hedw., *Sciurohypnum reflexum* (Starke) Ignatov & Huttunen, *Ptilidium pulcherrimum* (Weber.) Vain., *Brachythecium salebrosum* (Hoffm. ex F. Weber & D. Mohr) Schimp., *Callicladium haldanianum* (Grev.) H. A. Crum, and some others were found mainly on tree bases and the logs and stumps of initial stages of decaying. These species often grow on the bark of birch and are able to survive in relatively xeric environmental conditions of forest-steppe. The group of epixylic bryophytes growing on rotten wood of the last stages of destruction is not large. These species (*Blepharostoma trichophyllum* (L.) Dumort., *Lepidozia reptans* (L.) Dumort., *Lophoziopsis longidens* (Lindb.) Konstant. et Vilnet, *Lejeunea cavifolia* (Ehrh.) Lindb., *Dicranum flagellare* Hedw., *Tetraphis pellucida* Hedw., etc.) are typical mainly for old-growth spruce-fir and mixed forests (F5 and F6), where the proportion of epixylic species is higher (as compared with other forest types).

#### *Bryophyte Diversity in the Forests of the Southern Urals DOI: http://dx.doi.org/10.5772/intechopen.88301*


#### *Bryophytes*


#### *Bryophyte Diversity in the Forests of the Southern Urals DOI: http://dx.doi.org/10.5772/intechopen.88301*


#### *Bryophytes*


#### **Table 4.**

*Shortened synoptic table of bryophytes revealed in forest communities in the Southern Ural Mountains, Russia.*

The "real" epiphytes, growing on the bark of the living trees at a height of 1 m and above, are *Pylaisia polyantha* (Hedw.) Schimp., *Pseudoleskeella nervosa* (Brid.) Nyholm, *Orthotrichum obtusifolium* Brid., and *O. speciosum* Nees. These species are xero-mesophytic and often may be found on the trees of settlements and in contour strip forests surrounded by agricultural lands. There is another group of mesophytic epiphytes growing mainly on the bark of old broad-leaved trees. These species (*Leucodon sciuroides* (Hedw.) Schwaegr., *Neckera pennata* Hedw., *Homalia trichomanoides* (Hedw.) Brid., *Anomodon longifolius* (Schleich. ex Brid.) Hartm., *Dicranum viride* (Sull. & Lesq.) Lindb., and some others) are quite sensitive to the fluctuations in temperature and humidity of habitats. An analysis of the distribution of these species using ecological scales showed that they have a narrow ecological amplitude, especially in relation to the factor of continentality [25, 26]. Many of these species are basophilous and can grow on limestone outcrops. Probably, the

*Bryophyte Diversity in the Forests of the Southern Urals DOI: http://dx.doi.org/10.5772/intechopen.88301*

#### **Figure 2.**

*Substrate groups of bryophytes in the different forest types of the Southern Ural Mountains, Russia.*

presence of limestones helps preserve the local subpopulations of these species in the felling areas. The proportion of epiphytic species in the bryophyte flora of different forest types is more significant in broad-leaved and mixed pine broad-leaved forests (F3 and F4) (**Figure 2**).

Projective cover of epigeic bryophytes varies greatly depending on the forest type, being insignificant in broad-leaved forests (<1%) and reaching the higher values in boreal spruce-fir and pine forests (up to 80%) (**Table 1**). The high species richness of epigeic bryophytes in tall-herb dark coniferous and mixed forests (F5 and F6), as well as in floodplain alder forests (F1), may be explained by high humidity of soil in these habitats. In relatively dry habitats of thermophytic oak forests (F2) with dense herb layer and in shaded mesic linden-maple-oak forests (F3), the diversity of epigeic species is quite low (**Figure 2**).

Boreal species *Pleurozium schreberi* (Willd. ex Brid.) Mitt., *Hylocomium splendens* (Hedw.) Schimp., *Dicranum polysetum* Sw. ex anon., *Dicranum scoparium* Hedw., and *Rhytidiadelphus triquetrus* (Hedw.) Warnst. are almost completely absent in the oak forests (F2), occasionally occur in floodplain forests (F1), and are quite common in other forest types. In "typical" boreal green-moss coniferous forests (F7 and F8), they grow mostly on the forest floor, but in other forest types, these species were found mainly on logs, bases of trees, and stones where they avoid the competition with vascular plants.

In many forests of the Southern Urals, there are numerous rock outcrops. The proportion of epilithic species is high in the hemiboreal xero-mesophytic pine and pine-larch forests (F9) growing on steep southern and southeastern slopes with lime outcrops, where these species consist of 33% of bryophyte flora, but total species richness of epilithic bryophytes is highest in the dark coniferous forests (**Figure 2**).

#### **5. The peculiarities of bryophyte composition in the secondary forests**

Epiphytic, epixylic, and epilithic bryophytes are particularly responsive to microclimatic as well as physical and chemical substrate properties, which directly depend on tree age and diameter, bark texture, or decay stages of deadwood. Many bryophytes are sensitive to forest management practices [27, 28], but in Russia, the influence of felling on these components of forest vegetation has not been well studied [8].

A multitude of restoration practices are currently being used in the Southern Ural forests, but in spite of the government recommendations to establish in the felling areas the forest plantations through human-induced direct planting or seeding, the large felled areas are overgrown by deciduous pioneer tree species, for example, birch and aspen due to natural regeneration. Understanding how secondary forests differ from the indigenous forests in terms of diversity, structure, and function provides the basis for forest restoration ecology. The availability of mountain boreal forests in different stages of succession provides an opportunity for comparing of plant diversity and the structural and functional elements in the Ural's indigenous forests as well as in the secondary forests established after clear-cutting. Such data are crucial for planning monitoring and restoration of unique mountain forests [29].

In the Southern Urals, the study of reforestation processes has been only recently begun, but some preliminary results concerning dynamic of bryophyte diversity during natural forest regeneration were obtained [11, 26, 30].

The study of secondary plant communities originated after clear-cutting in the pine and broad-leaved forests [26, 30] has shown that bryophytes of various substrate groups respond to felling in different ways. Xero-mesophytic epiphytic and epixylic species (*Pylaisia polyantha* (Hedw.) Schimp., *Pseudoleskeella nervosa* (Brid.) Nyholm, *Brachythecium salebrosum* (Hoffm. ex F. Weber & D. Mohr) Schimp., *Amblystegium serpens* (Hedw.) Schimp., *Sciuro-hypnum reflexum* (Starke) Ignatov & Huttunen, and *Hypnum pallescens* (Hedw.) P. Beauv., etc.) seem to be more tolerant to habitat changes after felling. Usually, these species sharply reduce their constancy after felling but begin to grow actively after 3–4 years on stumps and felling residues and on the bases of young tree trunks. In secondary forests they restore or even increase their constancy.

The light-demanding colonists (*Ceratodon purpureus* (Hedw.) Brid., *Bryum caespiticium* Hedw., *Funaria hygrometrica* Hedw., *Pogonatum urnigerum* (Hedw.) P. Beauv., *Dicranella heteromalla* (Hedw.) Schimp, *Leptobryum pyriforme* (Hedw.) Wilson, and some others) have a relatively high constancy during the first 7–20 years after felling, but later they belong to the category of sporadically occurring species, growing on the soil near the roots of fallen trees and forest roadsides.

The nemoral species *Orthotrichum speciosum* Nees., *Leucodon sciuroides* (Hedw.) Schwaegr., *Dicranum viride* (Sull. & Lesq.) Lindb., *Neckera pennata* Hedw., *Homalia trichomanoides* (Hedw.) Brid., *Anomodon longifolius* (Schleich. ex Brid.) Hartm., *Oxyrrhynchium hians* (Hedw.) Loeske, and *Fissidens taxifolius* Hedw. are vulnerable to tree felling. They either disappear or have little constancy in the early succession communities.

Boreal species *Pleurozium schreberi* (Willd. ex Brid.) Mitt. and *Hylocomium splendens* (Hedw.) Schimp. are more resistant against changes in ecological regimes of habitats. *Ptilium crista-castrensis* (Hedw.) De Not., *Dicranum polysetum* Sw. ex anon., and *Rhytidiadelphus triquetrus* (Hedw.) Warnst. are more vulnerable [26].

In general, changing of bryophyte diversity in the felled areas seems to be as follows:

During 1–4 years after felling, the species richness is sharply reduced, in some cases by 50–70%. The cover of herb layer increases due to disturbances of the forest floor and high illumination. These processes are particularly intense after summer clear-cutting connected with strong disturbances of the forest floor. Usually, species richness of bryophytes begins to rise only after 7 years or more, when young trees appear and shading becomes more or less significant.

Nearly 60–90 years after felling, the similarity of the bryophyte composition in the indigenous and mature secondary forests still remains quite low due to the differences in species composition. Considering the fact that a significant number of bryophytes have a low abundance and scattered distribution, it is often difficult to say whether the disappearance of particular species is due to the felling or for some other reason.

#### **6. Rare bryophytes in the forests of the Southern Urals**

As mentioned above, about 25% of bryophyte species revealed in the sample plots within indigenous forests of the Southern Urals were found one to three times and formally may be considered as "rare" species. This group comprises *Riccia rhenana* Lorb. ex Muell. Frib., *Racomitrium aciculare* (Hedw.) Brid., *R. aquaticum* (Brid. ex Schrad.) Brid., *Odontoschisma denudatum* (Mart.) Dumort., *Tetraplodon angustatus* (Hedw.) Bruch & Schimp., *Philonotis seriata* Mitt., *Myrinia pulvinata* (Wahlenb.) Schimp., *Jungermannia pumila* With., *Rhizomnium magnifolium* (Horik.) T. J. Kop., *Brachythecium laetum* (Brid.) Schimp., and many other species, some of whom are not typical for the forests. Also, it should be noted that the small number of their known localities may be explained with insufficient botanical knowledge of the region. In this connection, the selection of indicator species for identifying forest areas that need protection constitutes the important challenge.

There are two aspects—the identification of indicator species of old-growth and primeval forests. Often, these two concepts are confused, which is not quite justified due to the differences in the life strategies of bryophytes and duration of the undisturbed existence of forests. The identification of areas where ecological conditions of habitats (especially humidity and illumination) were not changed for a very long time is very important for the conservation of bryophytes. Such areas are called zones of ecological continuity [31]. Not all old-growth forests are suitable for this purpose. Usually the term "old-growth forest" is defined as the forest in which the age of tree stand is more than 120–260 years (depending on the dominant tree species and the region) [8]. At the same time, the survival of relic populations of bryophytes having a limited dispersal activity and high demands on the stability of ecological regime in the habitats is possible mostly in primeval or ancient forests.

In authors' opinion, under selection of such criteria, both reproduction and the reasons of rarity of species should be taken into account. Among the bryophytes considered as indicators of old-growth forests in North-West European Russia [8], there are the species with different reproduction characteristics and dispersal ability. For instance, according to the system of life strategy [32], such species as *Homalia trichomanoides* (Hedw.) Brid., *Orthotrichum affine* Schrad. ex Brid., and *Haplocladium microphyllum* (Hedw.) Broth. may be considered as colonists and *Leucodon sciuroides* (Hedw.) Schwaegr., *Frullania bolanderi* Austin, and *Lejeunea cavifolia* (Ehrh.) Lindb. as shuttles. There are some species that may be considered as perennial stayers because of their low sporophyte frequency or lacking of sporophyte in study area but have vegetative propagules (*Dicranum viride* (Sull. & Lesq.) Lindb., *Barbilophozia attenuata* (Mart.) Loeske, etc.).

Most of these species are epiphytic or epixylic and can spread to a new site from the nearby forest area, if the suitable substrates are available. These species may be considered as indicators of old-growth forests. On the other hand, stayer species that have no vegetative propagules and prefer to grow on the soil or stones could be seen as indicators of both old-growth and primeval forests. This group includes the species that are very rare in the Southern Urals (*Eurhynchium angustirete* (Broth.) T. J. Kop., *Dicranum drummondii* Muell. Hal., *Entodon schleicheri* (Schimp.) Demet., *Plagiomnium confertidens* (Lindb. & Arnell.) T. J. Kop., etc.). Some of them may be considered as relicts.


*Note: Reproduction features: 1, sporophytes are frequent, and vegetative propagules are absent; 2, sporophytes are frequent, and vegetative propagules are present; 3, sporophytes are rare or unknown in study area, and vegetative propagules are present; 4, sporophytes are rare or unknown in study area, and vegetative propagules are absent. Reasons of rarity: 1, rare species growing at the border of the range (or with a disjunctive area); 2, species preferring to grow on old broad-leaved trees; 3, species growing on decaying wood of last stages of destruction; 4, sciophytic and hygrophilous species disappearing during the felling and clearing of the forests.*

#### **Table 5.**

*Hemerobic bryophytes in the forests of the Southern Ural Mountains, Russia.*

In **Table 5**, some species that may be considered as hemerobic in the Southern Urals are shown. It should be noted that the indicator value of these taxa is regional and may be different in the other areas where climatic conditions are not such continental as in the Southern Urals.

The forest associations characterized by high diversity and concentration of rare bryophytes were identified. There are mainly tall-herb and mixed forests (F5 and F6) where regionally rare species (*Brachythecium geheebii* Milde, *Eurhynchium angustirete* (Broth.) T. J. Kop., *Plagiomnium confertidens* (Lindb. & Arnell.) T. J. Kop., *Haplocladium microphyllum* (Hedw.) Broth., *Polytrichastrum pallidisetum* (Funck) G. L. Sm., *Iwatsukiella leucotricha* (Mitt.) W. R. Buck & H. A. Crum, and some others) were found. The proportion of rare species in these forests is about of 9%. In contrary, the bryophyte flora of pine forests seems to be well adapted to regular disturbances, that is, fires, and contain a significant number of species with high

*Bryophyte Diversity in the Forests of the Southern Urals DOI: http://dx.doi.org/10.5772/intechopen.88301*

reproduction activity. Also, these forests are characterized by a low number of rare species that consists of about 1% of bryophyte flora.

In the Pleistocene, refugia of the nemoral flora of the western slope of Southern Urals contained both the nemoral and the black taiga floristic elements [33]. Our previous research had shown some similarities between bryophyte flora of tall-herb broad-leaved dark coniferous forests of the Southern Urals and the black taiga of Salair Ridge in Siberia [11], as well as the availability of a significant number of relicts both of European and Asian origins in these forests. The sites of these forests are most valuable for nature conservation and should be protected.

#### **Acknowledgements**

The authors are grateful to the Russian Foundation for Basic Research for supporting of this study (grants no. 18-04-00641 and 16-04-00985-a) and governmental contract #075–00326–19–00 theme AAAA–A118022190060–6.

#### **Appendices and nomenclature**

The species names are given in accordance with the checklists for the territory of Russia [34, 35] and some later sources [36].

#### **Author details**

Elvira Baisheva\*, Pavel Shirokikh and Vasiliy Martynenko Institute of Biology—Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences, Ufa, Russia

\*Address all correspondence to: elvbai@mail.ru

© 2019 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/ by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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[30] Baisheva EZ, Shirokikh PS, Martynenko VB. Effect of clear-cutting on bryophytes in the pine forests of the South Urals. Arctoa. 2015;**24**:547-555

[31] Norden B, Appelqvist T. Conceptual problems of ecological continuity and its bioindicators. Biodiversity and Conservation. 2001;**10**:779-791. DOI: 10.1023/A:101667510

[32] During HJ. Life strategies of bryophytes: A preliminary review. Lindbergia. 1979;**5**:2-18

[33] Kamelin RV, Ovesnov SA, Shilova SI. Nemoral'nye relikty vo florakh Urala I Sibiri [Nemoral Relicts in the Floras of Ural and Siberia]. Perm': Perm' University; 1999. p. 82

[34] Ignatov MS, Afonina OM, Ignatova EA, et al. Check-list of mosses of East Europe and North Asia. Arctoa. 2006;**15**:1-130. DOI: 10.15298/ arctoa.15.01

[35] Konstantinova NA, Bakalin VA, et al. Checklist of liverworts (Marchantiophyta) of Russia. Arctoa. 2009;**18**:1-64. DOI: 10.15298/ arctoa.18.01

[36] Ignatov MS, editor. Moss flora of Russia. In: Oedipodiales—Grimmiales. Vol. 2. Moscow: KMK Scientific Press Ltd; 2017. p. 560

#### **Chapter 3**

## Species Distribution Patterns in Subgenus Cuspidata (Genus Sphagnum L.) on the East European Plain and Eastern Fennoscandia

*Sergei Yu. Popov*

### **Abstract**

The geographic range of 13 species from the subgenus Cuspidata in the East European Plain and Eastern Fennoscandia has been studied. Model maps for each species occurrence were constructed using geostatistics techniques (kriging method). Continuous coverages of 23 climatic factors were used in analysis also. We used dataset that proposed by authors of program WORLDCLIM. To learn how corresponding values of climatic factors and species occurrence correlation and cluster analysis were conducted. It was found that 7 of 13 species are widespread on the East European Plain and Eastern Fennoscandia, and 6 species have the restricted ranges. Values of occurrence of all species (except *Sphagnum lenense*) have a strong correlation with moisture factors (relative air humidity and sum of precipitation) in summer-autumn period. Such preferences allow them to grow successfully in Subarctic and Baltic regions, where high climatic humidity is observed. Restricted species are concentrated around the Baltic Sea and zones of the highest occurrence of widespread species are located at the same region. All species can be divided into four clusters according to its climatic preferences. Distribution of such species as *S. obtusum* seems to be strongly associated with two tongues of the Last Glacier, and this species seems to be a glacial relic.

**Keywords:** Sphagnum, Cuspidata, biogeography, BIOCLIM, distributional range, GIS, geostatistics, kriging method

#### **1. Introduction**

Sphagnum mosses are widely distributed plants in wet habitats. They are edificators in boggy forests and bogs in all plant zones. The ecology of species of the genus Sphagnum is now well studied, and environmental factors that play a leading role in the division of ecological space among Sphagnum species are well known [1–11]. Until now, however, the question about the division of geographical space by species remains open, especially due to the influence of climatic factors. There are two principal works on the biogeography of the genus Sphagnum [12, 13], which consider the geographical variability of species diversity of the genus in Western

Europe by methods of zonal statistics, that is, within the administrative boundaries of administrative states. In both cited works, the authors find the center of species diversity of the genus Sphagnum in the Scandinavian Peninsula. There does not seem to be any work that considers the distribution of species within its natural boundaries. Therefore, the present article is intended to fill this gap for the territory of the East European plain and Eastern Fennoscandia. As more than 50 species of Sphagnum grow in Europe [14], it is not possible to consider all of them in a single article due to lack of space. Therefore, in this chapter, we consider the distribution of species of the subgenus Cuspidata only, growing on the territory of the East European plain and Eastern Fennoscandia (EEPEF). In Europe (from the Atlantic to Urals), there are 17 species of the subgenus Cuspidata [14]. Only 14 species occur in the EEPEF. These are as follows: *Sphagnum angustifolium*, *S. annulatum*, *S. balticum*, *S. cuspidatum*, *S. fallax*, *S. flexuosum*, *S. jensenii*, *S. lenense*, *S. lindbergii*, *S. majus*, *S. obtusum*, *S. pulchrum*, *S. riparium*, and *S. tenellum*. Although some species are difficult to identify, these errors are easy to identify and correct by comparing bulk materials from different geographic locations. Moreover, a mathematical method for modeling maps, which is used in this work—the kriging method [15–20] serves as error protection. This method is widely used to build maps of temperature distribution in climatology, compiling digital elevation models in geodesy, etc. The advantages of this modeling method, compared to other ones currently used, are discussed in detail in previously published paper [21]. In bryology and biogeography, we use the kriging method for the first time. In short, the kriging method allows us to create model maps of the species distribution, which can reflect not only the boundaries of the species range as a whole, but also the species activity within the range. In addition, taking into account the weights of input points, values allow to cut off the noise while maintaining the overall trend of the distribution of the species. In the case of the study of mosses distribution, random incorrect definitions of species in some geographic points just appear as noise on a mathematical surface. All of the above is true for such species for which we have data set from the entire study area. Among the 14 species of the subgenus Cuspidata which is found in European Russia and adjacent countries, only one species does not satisfy this condition. This is *Sphagnum annulatum*. Since the valid description of this species was made relatively recently [22], and actually in Russian local floras, it "appears" around the late of 1990s, the definitions of this species cannot cover the entire study area (the database of local floras includes works, which were conducted since 1960s till 2017). In this connection, in the present work, 13 species of Sphagnum of the subgenus Cuspidata (from the list above), excluding *S. annulatum*, are analyzed.

The purpose of the present work is to simulate the ranges of species and study their distribution patterns, in connection with spatial changes of climatic factors in the EEPEF. In other words, it is completely within the competence of biogeography. The traditional task of biogeography is to identify the boundaries of the species ranges and find distribution patterns of the species due to geographic, biotic, and climatic factors. The ecological aspect of the species distribution analyzing in biogeography is most often associated with the concept of ecological niche in the understanding of Grinnell [23], that is, the attitude of a species to changes in environmental parameters. Unlike Hutchinson's ecological niche [24], which is determined by the properties of a species in the hyperspace of environmental factors (i.e., the ecological preferences of the species, rather than the environment), Grinell's niche is determined by environmental parameters. Changes of these parameters lead to changes of species environmental preferences. Therefore, studying the joint change of climatic factors and the numerical characteristics of the species in space, one can identify the climatic optimum and pessimum of the species.

*Species Distribution Patterns in Subgenus Cuspidata (Genus Sphagnum L.) on the East… DOI: http://dx.doi.org/10.5772/intechopen.84648*

#### **2. Methods**

To study the Sphagnum distribution on the EEPEF, 13 species were chosen, and the literature data with annotated lists of specific bryofloras from different regions (European part of the Russian Federation, the Baltic States, Ukraine, Belarus, and Moldova) were analyzed (**Figure 1**). Some dots have been chosen outside the study area (e.g., Romania, Poland, Kazakhstan, Cauacasus, and eastern mountainside of Ural) to correct possible errors of extrapolation at the boundaries [17, 20]. Earlier,

#### **Figure 1.**

*Study area, showing localities involved in analysis and vegetation zones: I—Tundra; II—Forest Tundra; III—Northern Taiga; IV—Middle Taiga; V—Southern Taiga; VI—Mixed forests; VII—Broadleaved forests; VIII—Forest Steppe; IX—Steppe; X—Semidesert; XI—Desert (boundaries of vegetation zones are given by [30, 31]. Study sites: 1–18—[32]; 19—[33]; 20—[34]; 21–23—[35]; 24—[33]; 25—[36]; 26—[33]; 27–29—[35]; 30—[37]; 31–32—[38]; 33—[39]; 34—[40]; 35—[41]; 36–39—[42]; 40—[43]; 41—[44]; 42–43—[45]; 44— [46]; 45—[32]; 46—[32, 47]; 47—[48]; 48–51—[49]; 52—[50]; 53—[51]; 54—[52]; 55–57—[53]; 58–59—[54]; 60–61—[55]; 62–64—[56]; 65–69—[27]; 70–72—[57]; 73–74—[58]; 75–77—[59]; 78–80—[60]; 81—[61]; 82—[62]; 83—[63]; 84—[64]; 85—[65]; 86—[66]; 87—[67]; 88—[68]; 89–92—[61]; 93–96—[69]; 97— [70]; 98–105—[71]; 106–108—[70]; 109–115—[57]; 116—[72]; 117—[73]; 118—[74]; 119—[75]; 120—[76]; 121—[77]; 122—[78]; 123–124—[79]; 125—[80]; 126—[81]; 127—[82]; 128—[83]; 129–130—[84]; 131—[85, 86]; 132—[87]; 133—[88]; 134—[89]; 135—[90]; 136—[91]; 137–138—[92]; 139–140—[93]; 141—[94]; 142— [95]; 143—[96]; 144—[97]; 145—[91]; 146—[98]; 147—[99]; 148—[100]; 149–158—[101]; 159–162—[40]; 163—[102]; 164–166—[103, 104]; 167—[105]; 168—[106, 107]; 169–171—[107, 108]; 172—[109]; 173—[110]; 174 —[94]; 175—[111]; 176—[112]; 177—[113]; 178—[114]; 179—[115]; 180—[116]; 181–182—[117]; 183— [75]; 184–188—[118]; 189—[77]; 190—[55].*

the basic principles for creating model areas by geostatistics methods using the kriging method were printed, and the methodology for compiling model maps of species ranges was adapted to the goals of biogeography [20]. After literature data compilation, the occurrence of each species was estimated in ordinal six-point scale: 0—absent (**abs**), 1—very rare (1–2 records) (**vr**), 2—rare (3–7 records) (**r**), 3 sporadically (more than 7 records, but not everywhere) (**sp**), 4—frequent (usual species, but sometimes absent in suitable phytocoenosis) (**fr**), and 5—common

*Species Distribution Patterns in Subgenus Cuspidata (Genus Sphagnum L.) on the East… DOI: http://dx.doi.org/10.5772/intechopen.84648*

(usual and phytocenotically active species in the study area) (**com**). In the following text, these abbreviations will be used to denote areas of species occurrence. According to this scale, continuous coverages were constructed for each species using the kriging method [17] with a resolution of 10 km in 1 pixel. In total, a sample of 190 points (local floras) was used to create continuous coverages (**Figure 1**). Verification of continuous coverages was carried out by cross-validation method in the SAGA GIS software. The index of quality of cross-validation in geostatistics is

#### **Figure 2.**

*Model ranges of 13 species of the subgenus Cuspidata (the red lines indicate the boundaries of vegetation zones). Zones of occurrence: abs—species is absent; vr—very rare; r—rare; sp—sporadic; fr—frequent; and com common. For each species on the maps is shown R2 .*

the coefficient of determination (R2 ) [17]. The values of this indicator for continuous coverages of species under study are shown in **Figure 2**. Climatic optimum was determined for zones of frequent (**fr**) and common (**com**) occurrences.

Continuous coverages of climatic factors were used in analysis also. We used dataset that authors of WORLDCLIM program [25] propose. In total, 23 climatic variables were used. This is the following: annual mean of precipitation (**amt**), monthly temperature of April–October (**tm04–tm10**), annual precipitation (**pr\_a**), monthly precipitation (**pr04–pr10**), and relative humidity (**reh04–reh10**) of April–October. We have chosen only months of growing season from dataset. Each coverage was composed in Azimuthal Equidistant Projection (Central Meridian 45°E, chief of the parallel 55°N). The coverages of climatic factors were combined with coverages of species occurrence to a single spatial database. This spatial database was converted into relative table, which contains 36 variables (23 climatic factors and 13 species occurrence) and 49,557 cases (number of pixels). This database was used for calculation of descriptive statistics and performing correlation and cluster analysis in software Statistica 10.0. Operation with creating and verification coverages was performed in SAGA software. The operations by intersection of the vector layers and calculating of areas were performed in software ArcGis 10.0. In more detail, all techniques were described in previous article [21].

#### **3. Results**

Model maps for 13 species are shown in **Figure 2**. The following species distribution patterns were found.

*Sphagnum angustifolium.* This species is widely distributed in the study area (**Figure 2**). The maximum score on the scale of occurrence is 5 (**com**). It grows in boggy forests and bogs. Its range is associated with the forest zone and tundra,


*Species Distribution Patterns in Subgenus Cuspidata (Genus Sphagnum L.) on the East… DOI: http://dx.doi.org/10.5772/intechopen.84648*

**Table 1.**

*Areas (in 1000 km<sup>2</sup> ) covered by S. angustifolium by zones of its occurrence.*

where such habitats are widespread. To the south of the forest zone, *S. angustifolium* decreases its abundance and completely disappears in the steppe or even in forest steppe in some places. Its occurrence increases in the northern and middle taiga. It grows in all vegetative zones from tundra to steppe (**Figure 2** and **Table 1**). The zone of its greatest occurrence (**com**) occupies 35.9% of the total area of the EEPEF. The zone of total absence is 23.7% (**Table 1**). Thus, the range of this species covers 76.3% of the total area of the EEPEF, therefore *S. angustifolium* can be considered here as a common and widespread species.

The southern boundary of the range of *S. angustifolium* (the southern boundary of **vr** zone) passes in sublatitudinal direction and is approximately parallel to the boundaries of natural zones. The border of the zone of maximum occurrence (**com**) passes diagonally to the meridians. In terms of biogeography, this is the zone of its climatic optimum. In the best way, the border of the **com** zone correlates with the boundary of the maximum occurrence of wetlands [26] and with isotherm of July +17°C and with maximal average values of air humidity in July–September.

*Sphagnum fallax.* This species is distributed from tundra to forest steppe zone (**Figure 2**). The maximum score on the scale of occurrence is 5 (**com**). In the south of the steppe zone, this species is absent, with the exception of its tongue with lower occurrence along Dnieper river, where it occurs on rare bogs, located on the river terraces [27]. It has maximal abundance (**com**) in the forest zone and occurs with a small abundance (**vr**) in the forest tundra and forest steppe, but here it is rare (**Table 2** and **Figure 2**). The zone of maximal occurrence of the species takes about a half area of the EEPEF (44.7%) (**Table 2**). This species is absent in 13.9% of the area only, that is, its range covers 86.1% of the EEPEF area. Thus, *S. fallax* is the most common and widespread species.

As well as *S. angustifolium*, *S. fallax* has similar climatic preferences. The boundaries of all zones of *S. fallax* are generally parallel to the boundaries of natural zones (**Figure 2**). Unlike *S. angustifolium*, *S. fallax* comes further south—its range reaches the Black Sea along the Dnieper. However, in the steppe zone, it is an extremely rare species. In the north of the EEPEF, *S. fallax* does not completely disappear, but becomes much more rare, in contrast to *S. angustifolium*, which is a fairly frequent species in the tundra (**Figure 2** and **Table 2**). The boundaries of all zones best correspond to region with the

greatest average summer precipitation and air humidity, and the southern border of its range is generally well suited the isotherm of July of +21°C (southern boundary of the **vr** zone) and to +13°C in the north (southern boundary of the **r** zone) (**Figure 2**).

*Sphagnum flexuosum.* This species is distributed from tundra to the steppe zone (**Figure 2**). The maximum score on the scale of occurrence is 4 (**fr**). It reaches the highest occurrence (**fr**) to the west of the forest zone (**Figure 2**), but it occurs sporadically throughout almost the entire forest zone. Sporadic zone occupies most of the range of this species −41.2%—and extends from the northern taiga to the forest steppe (**Table 3**). In general, *S. flexuosum* covers 80.7% of the total area, and therefore this species, as well as two previous species, can be considered as widespread species in this area.

The boundaries of almost all zones of occurrence of this species run almost parallel to the boundaries of natural zones (**Figure 2**). The boundary of the zone **fr** passes in the submeridianal direction. This fact indicates that the optimum zone of *S. flexuosum* is limited by the factors of humidity and not by temperature. This zone is located in regions around the Baltic Sea, where relatively warm summers and the greatest amount of precipitation are observed [28]. In the south, the range of this species reaches to the northern steppes only and in the north—to the Arctic Ocean. True, in tundra, it is very rare. In the best way, the boundaries of all zones of occurrence (except for zone **fr**) correspond to the high average values of precipitation in July–September, and they have a weak correspondence with isotherms (**Table 3**).

*Sphagnum balticum.* The range of this species from north to south covers the area from the tundra zone to the zone of deciduous forests, and its occurrence does not exceed four on a six-point scale (**Figure 2**). In the southern Urals, it captures a small section of the forest steppe zone (**Table 4**). The maximum occurrence of *S. balticum* is observed in the tundra and in the north of the forest zone. Zone **fr** occupies about a quarter of the total area (25.6%) of the EEPEF (**Table 4**). The territory, where *S. balticum* is absent (**abs**), makes up 38.6% of the EEPEF, that is, the range of this species occupies 61.4% of total area. Therefore, *S. balticum* can also be called a relatively widespread species.


#### **Table 2.**

*Areas (in 1000 km2 ) covered by S. fallax by zones of its occurrence.* *Species Distribution Patterns in Subgenus Cuspidata (Genus Sphagnum L.) on the East… DOI: http://dx.doi.org/10.5772/intechopen.84648*


#### **Table 3.**

*Areas (in 1000 km<sup>2</sup> ) covered by S. flexuosum by zones of its occurrence.*


#### **Table 4.**

*Areas (in 1000 km<sup>2</sup> ) covered by S. balticum by zones of its occurrence.*

The boundaries of the range of *S. balticum* as a whole and the boundaries of zones of occurrence within the range are oblique with respect to the borders of natural zones and show a clear tendency toward concentration around the Baltic Sea (**Figure 2**). The boundary of the zone of maximal occurrence (**fr**) lies parallel and entirely within the zone of maximum distribution of the Valdai glaciation [29]. The boundary of the zone of sporadic occurrence (**sp**) generally coincides with the zone of maximal distribution of wetlands [26]. This is not surprising if we recall that *S. balticum* is predominantly a boggy (and not forest) species, especially in the north [9–11]. Thus, it can be assumed that the distribution of *S. balticum* in the northern parts of its range, where it occurs most often, in addition to climatic

factors, is influenced by the historical conditions and landscape features of the territory. The influence of climatic factors, however, also occurs, since the southern border of the **sp** zone roughly corresponds to the isotherm of July +17°C. In the best way, the boundaries of the zones of occurrence correspond to the monthly precipitation and relative humidity of air in August–September.

*Sphagnum riparium.* It is rather widely distributed in the EEPEF (**Figure 2**); however, in most of the area, it occurs sporadically. The **sp** zone occupies about half of the investigated area (45.8%) (**Table 5**). The maximal occurrence zone reaches in Finland and Sweden (**Figure 2**), which is connected, in my opinion, with the greater prevalence of suitable habitats in these countries, such as aapa-bogs. In general, the *S. riparium* range covers 74.7% of the EEPEF, so this species can be considered widespread in this area.

In the west, the boundary of the **sp** zone more or less coincides with the isotherm of July +17°C. In the east—in the Ural Mountains—any correspondence to climatic factors is not detected. The decrease of the occurrence of *S. riparium* in Urals seems to be due to the lack of suitable habitats.

*Sphagnum majus* and *S. cuspidatum*. Both species, as well as *S. riparium*, are widely distributed throughout the EEPEF, but with a small abundance. The peak of their coenotic activity is observed in western regions, where they grow jointly or separately in the flooded hollows of oligotrophic or mesotrophic bogs. Apparently, their lower occurrence in the east is related to the difference in the composition of the bog complexes of the Western European and East European bogs. Although the ranges of both species are largely similar, *S. majus* is more northern than *S. cuspidatum*. Area of *S. majus* covers 66.8% and *S. cuspidatum*—59.7% (**Tables 6** and **7**) from total area. The boundaries of the zones of occurrence of both species are weakly related to the isolines of any climatic factors, except zone **fr**. This zone (for both species) lies within the region with the highest humidity and precipitation in August–September.

*Sphagnum jensenii*. The boundary of the range of this species has a fancy pattern. In general, it occupies 56.1% of the total area (**Table 8**). It is most prevalent in Fennoscandia and in Russian North (**Figure 2**). Throughout its range, *S. jensenii*


#### **Table 5.**

*Areas (in 1000 km<sup>2</sup> ) covered by S. riparium by zones of its occurrence.*

#### *Species Distribution Patterns in Subgenus Cuspidata (Genus Sphagnum L.) on the East… DOI: http://dx.doi.org/10.5772/intechopen.84648*


#### **Table 6.**

*Areas (in 1000 km<sup>2</sup> ) covered by S. majus by zones of its occurrence.*


#### **Table 7.**

*Areas (in 1000 km<sup>2</sup> ) covered by S. cuspidatum by zones of its occurrence.*

practically does not change its environmental preferences—it grows everywhere in the wet hollows of oligotrophic bogs. However, on the territory of the Russian Plain, such bogs are not rare, but wet hollows are usually occupied mainly by *S. majus*. Therefore, it cannot be said that *S. jensenii* is extremely rare due to the lack of habitats in the central and eastern parts of the range. At the same time, it cannot be said that the boundaries of the zones of occurrence are associated with isolines of climatic factors. This type of range appears to be shrinking.

*Sphagnum obtusum.* The maximum score on the scale of occurrence for this species is 3 (sporadically). In other words, this species does not have an optimum in the study area. At the same time, the **sp** zone "goes" to EEPEF with two tongues—from

#### *Bryophytes*


#### **Table 8.**

*Areas (in 1000 km2 ) covered by S. jensenii by zones of its abundance.*


#### **Table 9.**

*Areas (in 1000 km<sup>2</sup> ) covered by S. obtusum by zones of its occurrence.*

Finland and Polar Urals. This is very similar to the tongues of the last glacier [29]. This is a suggestion that this species is a glacial relic. In general, the area of this species is 66.4% of the total area of the EEPEF (**Table 9**). This species does not change its ecology when geographic areas changing—everywhere it grows on quagmire along the shores of lakes or in hollows of transitional bogs and rich fens. Therefore, in our opinion, the range of this species can also be called shrinking.

*Sphagnum lindbergii.* This species is quite rare on the Russian Plain. Judging by the pattern of its range—it is rather Scandinavian. The area of its range is less than half of the total area (38.7%) (**Table 10**). Therefore, this species should be considered as a species with a restricted range for the EEPEF territory. The zone of *Species Distribution Patterns in Subgenus Cuspidata (Genus Sphagnum L.) on the East… DOI: http://dx.doi.org/10.5772/intechopen.84648*


#### **Table 10.**

*Areas (in 1000 km<sup>2</sup> ) covered by S. lindbergii by zones of its occurrence.*


#### **Table 11.**

*Areas (in 1000 km<sup>2</sup> ) covered by S. pulchrum by zones of its abundance.*

its maximal distribution occurs in subarctic regions, where an air humidity is high during the growing season (**Table 11**).

*Sphagnum pulchrum* and *S. tenellum*. These two species go to the EEPEF from Western Europe and Scandinavia. In the investigated area, they have a restricted range. Thus, the area occupied by *S. pulchrum* is only 26.3% and by *S. tenellum*— 24.7% of the total EEPEF area (**Tables 11** and **12**).

*Sphagnum lenense*. This species is widespread in Siberia and goes to the EEPEF from the Polar Urals. Also, this is a species with a restricted range. The area of its range is only 5.1% of the total area (**Table 13**). In the tundra and forest tundra, it grows on the hummocks of raised bogs. Under the conditions of the Russian plain, in forest zone, such habitats are usually occupied by *Sphagnum fuscum*. The

#### *Bryophytes*


#### **Table 12.**

*Areas (in 1000 km2 ) covered by S. tenellum by zones of its occurrence.*


#### **Table 13.**

*Areas (in 1000 km<sup>2</sup> ) covered by S. lenense by zones of its occurrence.*

southern boundary of the **sp** zone approximately corresponds to the isotherm of annual mean temperature of −4°C and an annual precipitation amount of 500 mm.

#### **4. Discussion**

If we consider the model maps of species, constructed according to the value of occurrence, as their geographical range in the territory of the EEPEF, then the areas of occurrence identified on it indicate areas where mosses have optimal and pessimal conditions. Results show that almost all species have an optimal area in the regions around the Baltic Sea or in the subarctic, where the wettest conditions are observed in the EEPEF. If we express the values of the moisture factors necessary for *Species Distribution Patterns in Subgenus Cuspidata (Genus Sphagnum L.) on the East… DOI: http://dx.doi.org/10.5772/intechopen.84648*

the successful distribution of species, in absolute values, they look as follows: annual precipitation is not less than 550 mm and relative humidity is not less than 60–70%.

A total of 7 species of 13 are widespread in the study area. These are *S. angustifolium*, *S. fallax*, *S. flexuosum*, *S. balticum*, *S. riparium*, *S. majus*, and *S. cuspidatum*. All of them play an important phytocenotic role in wetlands. Restricted species have western distribution. And only *S. lenense* comes to the north of the European part of Russia from the east. Some of the restricted species, such as *S. obtusum* and *S. tenellum*, do not have an optimum in the EEPEF. This suggests that they come here only at the edge of the range, and the center of their distribution is outside the EEPEF. Abovementioned seven species are characterized by the largest phytocenotic significance in wetland communities. If we compare their ranges (**Figure 2**), it is clear that they overlap significantly, but, nevertheless, each species is characterized by its own characteristics. The *S. flexuosum* area pattern is the most different from the others. This species is practically absent in the tundra and reduces its abundance to the north and south of the forest zone. At the same time, it cannot be called the most "southern" of all seven species, since the range of *S. fallax*, for example, goes even further south than *S. flexuosum* (**Figure 2**). At the same time, *S. fallax* is able to grow in the tundra, that is, far north than *S. flexuosum*. Although *S. flexuosum* grows throughout the entire EEPEF forest zone, it is obvious that its western regions are under heavy rainfall conditions. The range of *S. angustifolium* in the southern part is similar to the pattern of the ranges of *S. fallax* and *S. flexuosum*. In the north, *S. angustifolium* comes much farther into the tundra and can be found there quite often, unlike the last two (**Figure 2**). The most northern species, perhaps, can be called *S. balticum*. On the southern limit of its range, it is limited to the southern boundary of the forest zone, and in the north, it is widely represented in taiga and in tundra. The orientation of the boundaries of its range is parallel to the boundary of the last glaciation and the zone of maximal spread of wetlands (and not the boundaries of natural zones). Such orientation of boundaries indicates that its distribution in the EEPEF is caused not only by climate parameters but also by the landscape structures that formed on the plain as they recede the glacier. This equally applies to *S. riparium*.

As correlation analysis shows (**Table 14**), the occurrence in the local floras of all species of the subgenus Cuspidata, except for *S. lenense*, *S. pulchrum*, and *S. tenellum*, has a high positive relationship with the rainfall of August (**pr08**), September (**pr09**), and October (**pr10**) (**Table 2**). According to WorldClim data [25], the maximum humidity in the EEPEF is observed in the west of forest zone and tundra zone during the summer-autumn season and sharply decreases in values starting from the south of the forest steppe zone, which is associated with an increase in monthly and average annual temperatures. Therefore, in the south, species of the subgenus Cuspidata quickly reduce their abundance, completely disappearing in the south of the steppe zone, or even further north (**Figure 2**). This is associated with high negative correlation coefficients between the values of occurrence and monthly temperatures (**tm**) of the vegetation period (**Table 14**). In the north, in the tundra, the occurrence of many species decreases, but not as sharply as at the southern limit of distribution. Apparently, despite the cold summer, they still find enough moisture here to grow successfully.

The cluster analysis conducted for 13 species of the subgenus Cuspidata by the values of 23 climatic factors shows that the studied species are divided into four clusters according to their climatic preferences (**Figure 3**). **First cluster:** *S. lenense*; **second cluster:** *S. tenellum*, *S. pulchrum*, and *S. lindbergii*; **third cluster:** *S. jensenii*, *S. obtusum*, *S. majus*, *S. cuspidatum*, and *S. balticum*; and **fourth cluster:** *S. fallax*, *S. flexuosum*, *S. angustifolium*, and *S. riparium*. It is interesting to note that within these groups, there is a similarity in environmental preferences also. So the species


*Species Distribution Patterns in Subgenus Cuspidata (Genus Sphagnum L.) on the East… DOI: http://dx.doi.org/10.5772/intechopen.84648*


*Values of r >0.5 in absolute value are highlighted in bold. All values are statistically significant at p < 0.05. Note: Climatic factors: amt—annual amount of precipitation; pr01–pr12—monthly amount of precipitation in January–December; pr\_a—annual precipitation average; reh4-reh10—relative humidity in April–October; and tm04–tm10—monthly temperature average in April–October.*

#### **Table 14.**

*The Spearmen correlation coefficient between the values of climatic factors and species abundance.*

#### **Figure 3.**

*Tree diagram of 13 species by 23 climatic factors.*

belonging to the 4th cluster grow mainly in the carpets of mesotrophic or oligotrophic bogs and boggy forests; the species belonging to the third cluster are most often found in heavily flooded hollows of bogs and fens; the species belonging to the second cluster grow in less flooded hollows of bogs. The *S. lenense* stands apart, which is found in the studied territory on hummocks in the boggy tundra. It seems to us that the similarity in the species relation to the conditions of watering of the habitat and climate is not accidental. The fact is that the amount of precipitation determines the hydrological regime in peat, and the humidity of the air affects the safety of the growing point during the dry season in the middle of summer.

#### **5. Conclusion**

Comparing the distribution ranges of 13 species of the subgenus Cuspidata in the EEPEF shows that there are as well as widespread and restricted species. The widespread species are as follows: *S. angustifolium*, *S. fallax*, *S. flexuosum*, *S. balticum*, *S. riparium*, *S. majus*, and *S. cuspidatum*. The restricted ones are *S. pulchrum*,

#### *Bryophytes*

*S. obtusum*, *S. jensenii*, *S. tenellum*, *S. lindbergii*, and *S. lenense*. Widespread species are common in wetland communities through entire area of the EEPEF in forest zone and tundra (except *S. cuspidatum*, which is absent in tundra). Restricted species (except *S. lenense*) have western trend in its ranges. Maximum activity (optimum) of these species depends on moisture factors (humidity and precipitations), and southern boundaries are limited by temperature. The only *S. lenense* is eastern (Siberian) species. It mainly occurs in tundras and one can see a middle dependence of its distribution on the temperature factors (**Table 14**).

#### **Author details**

Sergei Yu. Popov Lomonosov Moscow State University, Moscow, Russia

\*Address all correspondence to: sergei.popov.2015@yandex.ru

© 2019 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/ by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

*Species Distribution Patterns in Subgenus Cuspidata (Genus Sphagnum L.) on the East… DOI: http://dx.doi.org/10.5772/intechopen.84648*

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[116] Volkova LA, Kuzmina EO, Boch MS, Luknickaya AF, Chaplygina OY, Belyakova RN, et al. Mosses, algens and lichens of Nizhnesvirsky State Reserve. In: Flora and Fauna of State Reserves. Moscow: Nauka; 1996. p. 34

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**Chapter 4**

## Bryophytes: A Potential Source of Antioxidants

*Dheeraj Gahtori and Preeti Chaturvedi*

#### **Abstract**

A variety of degenerative diseases are caused by free radicals. Oxidative stress, the major cause of the diseases, is due to the imbalance between the free radicals and the antioxidants. To overcome this imbalance, the body needs antioxidants whether endogenously present or supplied from exogenous sources. Hence, the search of effective natural antioxidants is greatly needed to fight the onset of degenerative diseases and aging. Indeed, vascular plants are well-known sources of good and efficient natural antioxidants. Non-tracheophytes are however relatively unexplored. Interestingly, these atracheophytes are endowed with the remarkable property of desiccation tolerance which makes them unique in the plant kingdom. The property is attributed to its specialized structure and rich reservoir of phytochemicals. Therefore, there is a need to bioprospect this rich resource for antioxidants.

**Keywords:** bryophytes, antimicrobial, antioxidant, phytochemicals

#### **1. Introduction**

The use of medicinal plants for treating human ailments is as old as the mankind. Man's keen observations of the mother nature led to disclosure of various curative properties of plants. These properties were acquired by the plants during evolution as adaptive strategies for protecting against various abiotic and biotic challenges faced by the plants. Changing climatic conditions of the earth also played an important role in designing plant's adaptive abilities. Human has utilized these abilities of the plants for ensuring his own survival. Needless to say, before the introduction of modern medicine, disease treatment was mainly managed by herbal remedies. Plants were found to be a rich source of therapeutic agents and hence contributed to the drug industry for a long time. Today also, many important medicinal compounds are derived from plant sources. There is huge potential to further harness this resource by exploring the vast diversity present in the plant world [1].

With increase in awareness and people becoming more health conscious, their attitude toward medicine and diet has undergone a dramatic transformation. Now, there is increased focus on plant-based diet and healthcare supplements. The natural supplements are relatively healthier and free from side effects of harmful chemicals. In human body, different natural mechanisms are responsible for production of free radicals and other reactive oxygen species (ROS). These species perform dual functions, viz*.*, lethal as well as favorable, depending upon their concentrations. The delicate equilibrium between these two contrary effects needs to be maintained for a healthy life. At low or optimum levels, reactive oxygen species exert positive effects

on cellular redox signaling and immune function, but at higher concentration, they produce oxidative stress, which may be responsible for onset of many degenerative diseases, apoptosis, aging, and food rancidity [2]. Therefore, wholesome antioxidant diet or natural antioxidant supplements should be used for a healthy life. Further, the novel and nonconventional sources of these antioxidants need to be documented regularly for relaxing the dependence on traditional sources on the one hand and for utilization of potential sources in the future as well.

Earlier, the food habits of man were ensuring sufficient intake of antioxidants in the form of fresh fruits, vegetables, and spices. In the fast-food age, change in food habits led to insufficient supply of these antioxidants. Now again, a need is being felt to use more and more of antioxidants in our day-to-day diet. As of today, both synthetic and natural antioxidants are very commonly used in food industry for increasing shelf life and improving quality of food. Another major industry using these antioxidants is medicine where they are mainly used for developing dietary supplements to promote health effect. Besides, cosmetic industry and herbal therapeutics are also using different types of natural as well as synthetic antioxidants. Needless to say, in today's scenario, the use of synthetic antioxidants is diminishing due to increasing public awareness related to their long-term carcinogenic effect which has brought about strict legislation on their use as food additives. Nowadays, natural antioxidants are increasingly being preferred over their synthetic counterparts. Presently, the importance of the plant-based antioxidant constituents in providing protection against deadly diseases like cancer and heart problems as well as promoting overall health is increasingly being realized all over the world [3].

Phytochemicals derived from plants are major source of antioxidants. These phytochemicals are redox-active molecules and are dynamic to maintain redox balance in the body. Undoubtedly, plant-derived natural antioxidants are supposed to have more progressive effect on the body than synthetic ones. This is because plant constituents are a part of physiological functions of living flora and thus well suited to the human body. In recent years, the rising importance of biologically active components of plant origin has gained increased significance as highly promising prophylactic and restorative measures to combat diseases caused by oxidative stress. Higher plants, in particular, angiosperms, are used and explored as antioxidant sources. Cryptogams, especially bryophytes, hold rich reservoir of unique phytochemicals imparting them a strong defense mechanism to survive under highly diverse habitats despite having a non-lignified structure. There is huge potential to utilize this untrapped resource in modern healthcare as eco-friendly antibiotics and antioxidants [4].

Bryophytes, including liverworts, hornworts, and mosses, are phylogenetically placed between algae and vascular plants and form a unique division in the plant kingdom. They are small, mostly terrestrial, photosynthetic, spore-bearing plants that generally require a humid environment but can be found all over the world. These are represented by ca 7266–9000 liverworts, ca 221–225 hornworts, and 12,700–13,373 mosses [5, 6]. This large diversity of bryophytes also act as a "remarkable reservoir" of natural products or secondary compounds such as terpenoids, flavonoids, alkaloids, glycosides, saponins, anthraquinons, sterols, and other aromatic compounds. Many of them show interesting biological activity and become a potential source of different medicines. They also possess anticancer and antimicrobial activity due to their unique chemical constituents [7].

#### **2. What are antioxidants?**

The chemical reaction that can produce free radicals and leads to chain reactions that may damage the cells of organisms is known as the oxidation, and the

#### *Bryophytes: A Potential Source of Antioxidants DOI: http://dx.doi.org/10.5772/intechopen.84587*

compounds that inhibit or retard the oxidation of compounds are known as antioxidants. Antioxidants are broadly classified into three groups [8].


The chemical compounds and reactions which are capable in generating potential toxic oxygen species/free radicals are referred to as "prooxidants." They attack macromolecules including proteins, DNA, and lipids and cause cellular or tissue damage. In a normal cell, due to the result of imbalance between reactive oxygen species (ROS) and antioxidant defenses, the oxidative stress is generated. It can result in serious cell damage if the stress is massive or prolonged. This leads to improper functioning which causes different pathogenic conditions like aging, carcinogenesis, cardiovascular dysfunction, neurodegenerative diseases, etc.

The reactive oxygen species (ROS) is generated during the different essential processes like photosynthesis, respiration, and stress responses. These ROS can lead to the disruption of the normal physiological and cellular functions and also the biomolecules of plasma membranes and cell walls [10, 11]. Interestingly, there are both ROS producing as well as ROS quencher systems operational in various organelles of cell. Low levels of ROS are beneficial sometimes acting as signaling molecules for stress tolerance by causing upregulation of the genes involved in the pathway of synthesis of stress enzymes/metabolites. High concentration of ROS is, however, deleterious and needs to be scavenged by either the intake of antioxidants or body's own endogenous antioxidants.

#### **3. Antioxidant property in bryophytes**

Bryophytes constitute a group of small plants which form essential components of terrestrial ecosystems. These are moisture-loving plants found mostly at the sites where water is readily available [12]. Although nowadays these plants are increasingly being focused for therapeutic research, the backbone of therapeutics, i.e., the chemistry of the group, is too limited covering less than 10% of the bryophytes [13]. Bryophytes possess good biological activities. The diverse activities of the bryophytes ranged from antimicrobial, cytotoxic, antitumor, cardiotonic, allergy causing, irritancy and tumor effecting, insect anti-feedant, molluscicidal, piscicidal, plant growth regulatory to superoxide anion radical release inhibition and 5-lipoxygenase, calmodulin, hyaluronidase, and cyclooxygenase inhibitions [14].

Among all the bryophytes, liverworts, being remarkable reservoir of natural products, are therapeutically used worldwide, especially in Indian and Chinese systems of medicine for the treatment of hepatitis and skin disorders [15–17]. Mosses, though more diverse than liverworts, are relatively lesser explored for medicinal utility. The secondary metabolites identified from mosses belong to terpenoids,

flavonoids, and bibenzyls. They are also rich in other compounds such as fatty acids, acetophenols, etc. Their antimicrobial activity is related to the specific chemical composition, structural configuration of compounds, functional groups, as well as potential synergistic or antagonistic interactions between compounds [14].

Bryophytes produce a number of secondary metabolites that strengthen these delicate plants with strong antioxidative machinery to cope up with biotic and abiotic stresses [18, 19]. To compensate for the absence of any special morphological and anatomical defense mechanism, these plants have developed active molecular and chemical defenses for their protection. The antioxidant defenses provide protection to the cell membranes and cell organelles against oxidative damage. Under unfavorable conditions, reactive oxygen species react with important cell constituents, viz., proteins and lipids, causing disruption of cell structure ultimately leading to cell damage. Antioxidant enzymes protect cells against the oxidative stress induced by both internal and external unfavorable conditions. High level of these antioxidants present in liverworts and mosses can serve as a future source for medicinally and cosmetically significant compounds [20].

Several bryophytes have been reported to show significant antioxidant activity. Some of these bryophytes possessed very efficient antioxidant enzyme systems, while others showed the presence of diverse kinds of phenolics and flavonoid compounds responsible for free radical scavenging. In one such study on the liverwort *Marchantia polymorpha*, antioxidant enzyme peroxidase was characterized which was found to be different from any known peroxidase of vascular plants [21]. Similarly, a search for antioxidant enzymes in a moss, *Brachythecium velutinum*, and a liverwort, *M. polymorpha*, showed the role of an enzyme, ascorbate peroxidase, in the removal of hydrogen peroxide [22]. In another study, the extract of *Plagiochasma appendiculatum* showed significant antioxidant activity by inhibiting lipid peroxidation and increasing superoxide dismutase and catalase activity [23]. Reverse-phase high-pressure liquid chromatography reported the presence of various phenolic compounds such as caffeic, gallic, vanillic, chlorogenic, p-coumaric, 3-4 hydrozybenzoic, and salicylic acid in the moss *Sphagnum magellanicum* [24]. Other studies determined the presence of phenols, flavonoids, saponins, tannins, and glycosides in *M. polymorpha*. These studies also indicated anticancerous role of flavonoids extracted from cell suspension cultures of *M. linearis* against colon cancer cell lines [25, 26]. The biological characteristics of the terpenoids and aromatic compounds isolated from bryophytes also showed antibacterial and antifungal activities [27, 28]. Like other plants, antioxidant activity of bryophytes is influenced by several factors, viz., altitude, tissue type, and seasons [29]. The biochemical compounds responsible for antioxidant activity are also subject to quantitative and qualitative change in response to changes in these factors.

Bryophytes are traditionally used in the Chinese, Indian, and American societies for various medicinal purposes. However, the ethnomedicinal use of bryophytes needs to be scientifically investigated and validated for active principles in order to bridge the gap between traditional knowledge and pharmacology. For this, the active principle responsible for the specific activity may be identified and purified. The study on the antioxidant activities of the extracts of *Oxytegus tenuirostris*, *Eurhynchium striatum*, and *Rhynchostegium murale* showed that the climate is the most important ecological factor that determines the antioxidant property of the moss. Depending on these factors, antioxidant amounts in the species vary both within themselves and between species [30]. The study on the total free radical scavenging activity of *Eurhynchium striatulum* and *Homalothecium sericeum* showed that these have very strong free radical scavenging activity [31].

The alpine moss, *Sanionia uncinata*, produces some secondary metabolites that help the plant against the environmental stresses such as UV, drought, and high

#### *Bryophytes: A Potential Source of Antioxidants DOI: http://dx.doi.org/10.5772/intechopen.84587*

temperatures. *S. uncinata* shows good antioxidant activity, free radical scavenging activity, reducing power, superoxide radical scavenging activity, and ABTS [2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)] cation scavenging activity [32]. A study on the extracts of *Polytrichastrum alpinum* revealed that isolated compounds have two to sevenfold increased antioxidant activity than their extracts [33]. The reducing power of plant extracts was reported to be directly correlated with their antioxidant activity [34] and is based on the presence of reductones, which exert antioxidant activity by breaking the free radical chain and donating a hydrogen atom [35].

The remarkable nature of polyphenolics in terms of antioxidant potential has been identified to cure many lifestyle diseases [36]. Polyphenolic molecules contain one or many aromatic rings with hydroxyl groups. Generally, the antioxidant capacity of the phenolics is directly related with the number of free hydroxyls and conjugation of side chains with the aromatic rings [37]. The phytochemical studies on *Thuidium tamariscellum* showed the presence of significant level of terpenoids in the moss. High antioxidant property shown by the plant is reported to be mainly due to the presence of considerable amount of terpenoids [38].

Studies also revealed that the total flavonoid contents of liverworts were generally higher than those of mosses. Acrocarpous mosses had generally higher values of these compounds than that of pleurocarpous mosses. The total flavonoid contents of bryophytes growing at lower light levels were higher than those growing in fullsun. Likewise, total flavonoid contents of epiphytic bryophytes were highest, while those of aquatic bryophytes were the lowest. Species growing at low-latitudes had higher flavonoid content than those at high latitudes [39]. Studies also revealed that the antioxidant values of liverworts were closer to those of vascular plants. Guaiacol peroxidase and catalase activity of *P. appendiculatum* was found higher than *Pellia endivaefolia*, while superoxide dismutase, ascorbic acid, proline, glutathione, and total phenols were found higher in *P. endivaefolia* than *P. appendiculatum* [40].

Antioxidant and free radical scavenging activities are in the focus of attention of both medical practitioners and dieticians. Free radicals are supposed to play a key role in the pathogenesis of many diseases [41]. Oxidation processes may also decrease the stability of drugs and foods. Reactive oxygen species (ROS) and reactive nitrogen species (RNS) have been recognized as fundamental components of stress signal cascades [42] under both abiotic and biotic stresses [43, 44]. Bryophytes occupy a special position among plants because the haploid gametophyte dominates their life cycle. Some species have been studied for their tolerances to drought and water stress (flooding) [45, 46] or high nitrogen concentrations [47]. Mosses are common in the vegetation of all continents, but they are still highly marginalized in traditional medicines. The plants which can respond and adapt to drought stress are certainly better equipped with complex and highly efficient antioxidative defense systems comprising of protective nonenzymatic as well as enzymatic mechanisms that efficiently scavenge ROS and prevent damaging effects of free radicals [48].

Some crude extracts of mosses contain hypnogenols, biflavonoids, dihydroflavonols, polycyclic aromatic hydrocarbons, and hydroxy flavonoids [49–51]. Flavonoids are synthesized by plants in response to the microbial infection. This action is probably due to their ability to complex with extracellular and soluble proteins and to complex with bacterial cell wall [49]. A large number of bryophytes are used as medicines in alternative medicine system. **Table 1** enlists certain medicinal bryophytes having significant antioxidant potential (selected on the basis of studied literature) available.

The screening for the antioxidant property by DPPH and ABTS assays revealed slightly higher antioxidant activity in ethyl acetate extract of *M. polymorpha* than ethanolic extract. Luteolin was an important antioxidant compound present in the


#### **Table 1.**

*List of some bryophytes and their reported compounds showing antioxidant activity.*

extract apart from other phenolics and bis(bibenzyls) [16]. Similarly, glutathione was observed as an important antioxidant compound in the terrestrial moss, *Pseudoscleropodium purum*, growing in industrial environments which can be used as a biomarker for pollution monitoring [69]. Besides the above listed plants, there are several other bryophytes which are having significant antioxidant potential [70–72]. All these bryophytes could be explored further for purification of the bioactive components for future applications.

### **4. Conclusion**

Natural antioxidants form a promising alternative for synthetic antioxidants in food, cosmetic, and therapeutic industries. Easy availability, low cost, and lack of any harmful effects on the human body make natural antioxidants much sought after source of nutraceuticals. These antioxidants which are naturally present in many plant products, viz., fruits, vegetables, and spices, are remarkable reservoirs of radical quenchers. Increasing incidences of diseases vis à vis soaring pollution on the earth necessitates the use of natural therapeutic antioxidants as regular dietary supplements for providing better and efficient healthcare. Earlier, the focus of the world scientific community was on angiosperms as popular source of antioxidants. Nowadays, there is seen a paradigm shift of scientific focus from conventional and traditionally overexploited plant sources to nontraditional and nonconventional herbs. One such group of plants holding great potential can be desiccation-tolerant bryophytes that are usually considered not so useful plants by the layman community. Interestingly, due to storage of rich biomolecules, these desiccation-tolerant plants can also serve as an efficient source of many such antioxidants which could be used for novel drug discovery.

### **Author details**

Dheeraj Gahtori1 and Preeti Chaturvedi2 \*

1 Department of Botany, Government Post Graduate College, Uttarakhand, India

2 Department of Biological Sciences, G.B. Pant University of Agriculture and Technology, Uttarakhand, India

\*Address all correspondence to: an\_priti@yahoo.co.in

© 2019 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/ by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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#### *Bryophytes*

of a *Rhodobryum roseum* extract and its active components in isoproterenolinduced myocardial injury in rats and cardiac myocytes against oxidative stress-triggered damage. Pharmazie. 2009;**64**(1):53-57

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#### Chapter 5

## Ohioensins: A Potential Therapeutic Drug for Curing Diseases

Satish Chandra, Dinesh Chandra and Arun Kumar Khajuria

#### Abstract

Benzonaphthoxanthenones are a class of flavanoids which are absent in the liverworts and hornworts and present only in the mosses. Ohioensins are benzonaphthoxanthenones which are isolated from various moss species. First compound of this series was isolated from the Polytrichum ohioense Renauld & Cardot. and hence named as Ohioensin A. Together with Ohioensin A, there are 10 other Ohioensins (B–H) and their derivatives have been extracted from different species of mosses. These compounds are pharmaceutically very important and various studies have shown their usefulness as antioxidant, in Atherosclerosis and cytotoxic activities against various human tumor cell lines. In this chapter, synthesis of Ohioensins, their structure and potential medicinal uses are discussed.

Keywords: atherosclerosis, cancer, cytotoxic activity, moss, Polytrichum

#### 1. Introduction

Bryophytes are tinny nonvascular plants on the earth. They are classified into three phyla Bryophyta (mosses), Marchantiophyta (liverworts) and Anthocerotophyta (hornworts) and represented by 14,000, 6000 and 300 species respectively. Various compounds have been isolated from different species of bryophytes which show antifungal, antiviral, antibacterial, allergic contact dermatitis, anti-HIV, plant growth regulatory, cytotoxic, insecticidal, nitric oxide (NO) production, superoxide anion radical release inhibitory, neurotrophic, muscle relaxing, antiobesity, piscicidal, and nematocidal activities [1].

Among the bryophytes liverworts species possess cellular oil bodies. These are single unit membrane-bound cell organelles that contain ethereal terpenoids and aromatic oils suspended in proteinaceous matrix. Oil bodies are useful in taxonomy and chemosystematics of the liverworts. However, their origin, development and function in the plant is poorly understood. More than 1000 secondary metabolites have been reported from oil bodies and which are considered to be useful in medicine and various other activities. However, these organelles have not been reported in the species of mosses and hornworts [1, 2]. Due to absence of the oil bodies mosses possess comparatively less secondary metabolites. Though, various compounds: monoterpenoids, diterpenoids, triterpenoids, steroids, carotenoids, aromatic compounds (cinnamic acid, benzoic acid, flavones, isoflavones, biflavones, aurones, anthocyanins, benzonaphthoxanthenones) and their

derivatives, alkanes and related compounds, fatty acids (propionic, butylic, valeric, caproic, isovaleric, phenylacetic, cyclohexanecarboxylic acid, palmitic acid, eicosatetraenoic acid and octadecadienoic acid etc.), plant hormones (isopentenyl) adenine (2iP), indole acetic acid) pheophytins and phytochelatins have been isolated from different species of the mosses [3].

Flavonoids are common aromatic compounds present in the mosses and nearly 73 flavonoids and their glycosides have been isolated from different moss species [1]. Benzonaphthoxanthenones are a class of flavanoids only present in the mosses and have been isolated from various moss species [4]. Among Ohioensins, Ohioensin A was first compound isolated from the Polytrichum ohioense Renauld & Cardot. and hence named as Ohioensin A [5].

#### 2. Ohioensins

Ohioensins compounds are isolated from various moss species. Chemical formula and source of the compounds are summarized in Table 1 and their structures are mentioned in Figure 1.


Table 1.

Source and formula of Ohioensins and their derivatives.

#### 3. Synthesis of Ohioensins

Zheng et al. [6] proposed synthesis pathway of Ohioensins. They suggested that these compounds are synthesized from the condensation of o-hydroxycinnamate and hydroxylated bibenzyls. Their synthetic pathway is summarized in Figure 2.

Ohioensins: A Potential Therapeutic Drug for Curing Diseases DOI: http://dx.doi.org/10.5772/intechopen.81583

Figure 1. Structure of Ohioensins and their derivatives.

Figure 2. Synthesis of Ohioensins.

### 4. Pharmaceutical properties

#### 4.1 Cytotoxic activity

Compounds extracted from different species of bryophytes have shown cytotoxic activity against various cancer cell lines as: P-388 murine leukemia tumor, squamous carcinoma (KB), lung carcinoma (A549), breast ductal carcinoma (MDA-MB-435), liver hepatoblastoma (HEP-G2), and colon adenocarcinoma (LOVO) cell lines, glioma A172 cells, U87 glioma, T98G, osteosarcoma U2OS, leukemia HL-60, K562, MDR K562/A02 and MCF-7 breast cancer etc. These compounds induce apoptosis and necrosis through activation of caspases (a family of cysteine aspartic proteases), DNA fragmentation, activation of p38 (mitogenactivated protein kinase), nuclear condensation, proteolysis of poly (ADP-ribose) polymerase (PARP) and inhibition of antiapoptotic nuclear transcriptional factorkappa B, etc. These mechanisms play important role in the maintenance of the cell population size and apoptosis of cell in vivo [10]. Ohioensins also show cytotoxic activities against various cancer lines.


#### 4.2 Protein inhibitory activity

Ohioensin A (IC50 4.3 0.3 μM), Ohioensin C (IC50 7.6 0.7 l μM), Ohioensin F (IC50 3.5 0.2 μM), Ohioensin G (IC50 5.6 0.7 μM) compounds inhibits the activity of Protein tyrosine phosphatase 1B (PTP1B) in a dose-dependent manner [7].

#### 4.3 In the treatment of atherosclerosis

Atherosclerosis disease is characterized by the disrupted balance and abnormal accumulation of lipids, inflammatory cells, matrix deposits and smooth muscle cell

#### Ohioensins: A Potential Therapeutic Drug for Curing Diseases DOI: http://dx.doi.org/10.5772/intechopen.81583

proliferation in the wall of medium- and large caliber arteries [12]. Arteries are composed of an outer layer (also known as adventitia), a tunica media (made up of layers of smooth muscle cells) and interior layer (also called tunica intima) lined with endothelium. During normal conditions balance between the concentrations of nitrogen oxide (NO, act as vasodilator), and Endothelin-1 (ET-1, act as vasoconstrictor) in the arteries is maintained and the endothelium is shielded from inflammation, injury and thrombosis. Moreover, in such conditions leukocytes could not bind to the endothelium, smooth muscle cells (SMCs) not proliferate and platelet aggregation is minimized. However, during atherosclerosis NO production is inhibited and protection conferred on the endothelial cells is removed [13]. Subsequently endothelium is exposed to leukocytes and SMCs begins to proliferate. Excessive fat in the diet or genetic disorders cause increase in cholesterol and saturated fat in the blood. The low density lipoprotein (LDL) assembles on the proteoglycan of the endothelium and bind together to form aggregates. LDL become highly susceptible for chemical modification and oxidation after aggregation. Oxidation is usually brought by lipoxygenases of infiltrating leukocytes (monocytes and T-lymphocytes), NADH/NADPH oxidases of vascular cells. Procoagulant properties are increased and anticoagulant properties are inhibited when LDL oxidized. Oxidized LDL also increase adhesiveness of leukocyte to the endothelium. When atherosclerosis set in endothelium expresses intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and P-selectins as leukocyte adhesion molecules. Furthermore, tumor necrosis factor α (TNF-α) a cytokine, induces expression of ICAM-1 and VCAM-1 on vascular cells [14]. Hence for curing the atherosclerosis, expression of ICAM-1 and VCAM-1 needs to be down regulate or block for inhibiting interaction between leukocytes and vascular cells [15].

Ohioensin F, prevents TNF-α-stimulated expression of VCAM-1 and ICAM-1 and subsequently reduces monocyte adhesion to Vascular smooth muscle cells (VSMCs). Effects of Ohioensin F also suppress ROS production, MAPK pathways, Nuclear factor-κB (NF-κB) and Protein kinase B (Akt) activation. Thus, Ohioensin F inhibits expression of adhesion molecules which may provide a new therapeutic strategy for the treatment of atherosclerosis [16]. This property of the compound already has been patented [17].

#### 4.4 Antioxidant activity

Ohioensin F and Ohioensin G isolated from the methanolic extract of Polytrichum alpinum showed potent antiradical activities against 2,2<sup>0</sup> -azino-bis(3 ethylbenzothiazoline-6-sulphonic acid (ABTS• + ) and 2,2-Diphenyl-1-(2,4,6 trinitrophenyl)hydrazyl (DPPH) free radicals. The compounds (ohioensin F and ohioensin G) and the crude methanolic extract converted DPPH into DPPH-H by donating a hydrogen atom and inhibited the production of the chromogen cation of ABTS. Ohioensin F, ohioensin G and methanolic extract showed Fe3+ to Fe2+ reducing capacity and also show moderate activity against free radical nitric oxide (NO) in dose-dependent manner [18].

#### 5. Conclusions

Nearly all Ohioensins show cytotoxic activities against various cell lines, thus these can be used in the treatment of the various Cancer. Though, more research is needed in this aspect of the Ohioensins. Ohioensin F play important role in the

treatment of atherosclerosis and act as strong antioxidant. However, further investigation for therapeutic potential of these compounds is warranted.

### Conflict of interest

Authors show no conflict of interest.

#### Author details

Satish Chandra<sup>1</sup> \*, Dinesh Chandra<sup>2</sup> and Arun Kumar Khajuria<sup>3</sup>

1 Department of Botany, Government Degree College Tiuni, Dehradun, Uttarakhand, India

2 Uttarakhand Biodiversity Board, Dehradun, Uttarakhand, India

3 Department of Botany, H.N.B. Garhwal University Pauri Campus, Uttarakhand, India

\*Address all correspondence to: satishchandrasemwal07@gmail.com

© 2019 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/ by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Ohioensins: A Potential Therapeutic Drug for Curing Diseases DOI: http://dx.doi.org/10.5772/intechopen.81583

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novel compound for preventing or treating arteriosclerosis. Available from: https://patents.google.com/patent/ WO2013058632A3/en

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*Edited by Marko S. Sabovljević and Aneta D. Sabovljević*

Bryophytes, a group of plants present in all terrestrial biomes of the Earth, play a significant role in ecosystems and have potential use in many life domains. They can be used in the cosmetic, pharmaceutical, and healthcare domains and can help to improve air quality, create bio-repellents and bio-pesticides, and help cure both human and animal diseases. This book discusses novel aspects of fundamental and applicative bryophyte biology.

Published in London, UK © 2020 IntechOpen © HannaGottschalk / iStock

Bryophytes

Bryophytes

*Edited by Marko S. Sabovljević* 

*and Aneta D. Sabovljević*