**7.3 Biochemical analysis**

It has been reported that the Khari/Hill goats across the mid-hill region from east to west of the country has three distinct types with respect to genetic distance. The goats of the midwestern are bigger in body size followed by western region with eastern region having a smaller body size. Also a report on protein analysis indicated that hemoglobin was polymorphic in Khari/Hill goats with two genotypes, HbAA and HbAB, in the sampled population. The gene frequency of HbA was higher than HbB, which was more in the goats sampled from eastern Nepal. Also, it has been reported that the four genotypes of transferrin, TfAA, TfAB, TfBB, and TfAC, were found in the Khari/Hill goats with decreasing frequencies. The gene frequency of TfA was the highest followed by TfB and TfC. The gene frequencies of TfB and TfC were higher in the goats of eastern Nepal. Both polymorphisms of these two principal blood proteins including differences in gene frequencies between the populations of Khari/Hill goats found in different locations indicated the genetic variation in Khari/Hill goats [38].
