5.2.1.2 Staphylococcal pyoderma

Skin biopsy specimen from Staphylococcus aureus-induced pyoderma reveals diffuse dermal infiltration of neutrophils and macrophages. The patient's own serum detected coccoid bacteria phagocytized by the inflammatory cells (Figure 25) [20, 21, 23].

#### Figure 25.

Staphylococcus aureus-induced pyoderma (left, H&E; right, reactivity with patient's own serum). The dermis reveals diffuse infiltration of neutrophils and macrophages. The patient's own serum detects coccoid bacteria phagocytized by the inflammatory cells (arrows).

5.2.1 Bacterial infection

5.2.1.1 Tsutsugamushi disease (scrub typhus)

High-titer sera were obtained from other immunocompetent patients.

Patients' sera applicable to immunostaining in paraffin sections.

Blastocystosis (cell block of cultured

microbes)

\*

90

Table 4.

Infectious disease Serum

Immunohistochemistry - The Ageless Biotechnology

dilution

Staphylococcal pyoderma 1: 500 Staphylococcus aureus cultured Propionibacterium acnes folliculitis 1:500 P. acnes-specific antigen PAC3

Cutaneous sporotrichosis 1:500 Sporotrichin reaction positive Malassezia (Pityrosporum) folliculitis 1:500 Multiple skin papules on the chest Cutaneous alternariosis 1:500 Long-lasting skin lesion on the knee

Cutaneous cryptococcosis in leukemia 1:10 Chemotherapy with steroid

Hemorrhagic varicella 1:500\* Bone marrow transplanted acute

Cutaneous leishmaniasis (African type) 1:1000 High immunofluorescence titer Cutaneous leishmaniasis (Indian type) 1:1000 High immunofluorescence titer Visceral leishmaniasis (liver biopsy) 1:500 High immunofluorescence titer

Amebic dysentery 1:500\* High immunofluorescent titer Balamuthia encephalitis 1:500 Balamuthia DNA identified by PCR Cerebral toxoplasmosis 1:1000\* High immunofluorescence titer Cryptosporidiosis in AIDS 1:1000\* High immunofluorescence titer Duodenal cystoisosporiasis 1:500 Chronic intractable diarrhea

Acanthamebic encephalitis 1:500 Opportunistic infection in liver cirrhosis

Cutaneous gnathostomiasis 1:500 Creeping disease on the abdominal skin Extra-gastrointestinal (omental) anisakiasis 1:500 Positive with monoclonal antibody Liver ascariasis (surgical material) 1:500 Ouchterlony's diffusion-in-gel test

Japanese schistosomiasis (colon) 1:200 Calcified ova seen (healed remote case) Bilharziasis (colon biopsy) 1:500 Spine-forming ova seen in urine Multilocular echinococcosis 1:200 Hepatectomy specimen in Hokkaido

Tsutsugamushi disease (scrub typhus) 1:100 Immunoelectron microscopy performed

Comments

identified

administration

1:500\* High immunofluorescence titer

positive

Island

leukemia

Mite-borne Tsutsugamushi disease or scrub typhus endemic in Japan is caused by Orientia tsutsugamushi belonging to the family Rickettsiaceae [46]. Biopsy was performed from the mite-bite eschar, and hemophagocytic macrophages were clustered at the base of the ulcer. The diluted patient's serum gave positive granulated

signals in the cytoplasm of the macrophages [23], and pre-embedding

Neurocysticercosis 1:20 Multiple brain nodules
