5.2.4.6 Cystoisosporiasis

Cystoisospora (formerly called Isospora) belli infection causes intractable diarrhea in individuals who live in or have traveled to tropical poor sanitary areas. Infection in immunocompromised patients, particularly AIDS patients in Africa, is lifethreatening [61]. C. belli-induced acalculous cholecystitis is a noteworthy finding [62]. Duodenal biopsy specimen was taken from a Japanese male patient with adult T-cell leukemia in Kyushu Island. Chronic active inflammation with villous atrophy represented intractable diarrhea seen in this patient. Large-sized schizonts of C. belli were observed among the columnar cells in H&E preparations, and they were visualized by immunostaining using the patient's own serum (Figure 37) [21, 23].

morphology and size variation are characteristic [63]. We encountered a patient with diarrhea and high-titer serum antibody to B. hominis [64]. The target of immunostaining was formalin-fixed, paraffin-embedded cell block preparations of cultured B. hominis.

Cystoisosporiasis (left, H&E; right, reactivity with patient's own serum). Duodenal biopsy specimen was taken from a Japanese male patient with adult T-cell leukemia in Kyushu Island, complaining of severe diarrhea. Large-sized schizonts of Cystoisospora belli are scattered among the columnar cells (arrows). Immunostaining

Low-Specificity and High-Sensitivity Immunostaining for Demonstrating Pathogens…

Infestation of Gnathostoma happens after eating undercooked or raw freshwater fish, frogs, birds, and reptiles, and migratory swelling of the skin (creeping disease) follows [65]. Cut surface of G. hispidum was identified in targeted abdominal skin biopsy from creeping disease, seen in a Japanese male patient aged 60's. The larval body is histologically featured by spiculated cuticles, well-developed muscle cells

Blastocystosis (cultured Blastocystis hominis in cell block preparation: left, H&E; right, reactivity with the serum of a patient with serologically proven high-titer IgG). Cultured B. hominis reveals vacuolated morphology and size variation. The centrifuged pellet was fixed in formalin and embedded in paraffin to prepare cell blocks. The cell wall of the microbes is immunostained with the serum of a patient with diarrhea

Cell wall positivity was clearly observed, as shown in Figure 38 [21–23].

5.2.5 Helminthic infection

using patient's own serum decorates the microbe.

DOI: http://dx.doi.org/10.5772/intechopen.85055

5.2.5.1 Gnathostomiasis

Figure 37.

Figure 38.

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and high-titer serum antibody to B. hominis.
