Immunohistochemistry - The Ageless Biotechnology


immunoelectron microscopy employing a paraffin section disclosed labeled

Mite-bite eschar in Tsutsugamushi disease or scrub typhus (left, H&E; center, reactivity with patient's own serum; right, pre-embedding immunoelectron microscopy using paraffin section). Hemophagocytic macrophages are clustered at the base of eschar. The patient's serum gave positive granulated signals in the cytoplasm of the macrophages (arrows), and pre-embedding immunoelectron microscopy disclosed densely labeled granular

Low-Specificity and High-Sensitivity Immunostaining for Demonstrating Pathogens…

DOI: http://dx.doi.org/10.5772/intechopen.85055

diffuse dermal infiltration of neutrophils and macrophages. The patient's own serum detected coccoid bacteria phagocytized by the inflammatory cells

Skin biopsy specimen from Staphylococcus aureus-induced pyoderma reveals

Staphylococcus aureus-induced pyoderma (left, H&E; right, reactivity with patient's own serum). The dermis reveals diffuse infiltration of neutrophils and macrophages. The patient's own serum detects coccoid bacteria

granular material measuring 2 μm (Figure 24) [47].

5.2.1.2 Staphylococcal pyoderma

(Figure 25) [20, 21, 23].

Figure 25.

91

phagocytized by the inflammatory cells (arrows).

Figure 24.

material measuring 2 μm.

#### Table 4.

Patients' sera applicable to immunostaining in paraffin sections.

#### 5.2.1 Bacterial infection

#### 5.2.1.1 Tsutsugamushi disease (scrub typhus)

Mite-borne Tsutsugamushi disease or scrub typhus endemic in Japan is caused by Orientia tsutsugamushi belonging to the family Rickettsiaceae [46]. Biopsy was performed from the mite-bite eschar, and hemophagocytic macrophages were clustered at the base of the ulcer. The diluted patient's serum gave positive granulated signals in the cytoplasm of the macrophages [23], and pre-embedding

Low-Specificity and High-Sensitivity Immunostaining for Demonstrating Pathogens… DOI: http://dx.doi.org/10.5772/intechopen.85055

Figure 24.

Mite-bite eschar in Tsutsugamushi disease or scrub typhus (left, H&E; center, reactivity with patient's own serum; right, pre-embedding immunoelectron microscopy using paraffin section). Hemophagocytic macrophages are clustered at the base of eschar. The patient's serum gave positive granulated signals in the cytoplasm of the macrophages (arrows), and pre-embedding immunoelectron microscopy disclosed densely labeled granular material measuring 2 μm.

immunoelectron microscopy employing a paraffin section disclosed labeled granular material measuring 2 μm (Figure 24) [47].
