4.12 Application to rhinoscleroma

Corynebacterium kroppenstedtii, and the lesion is microscopically featured by lipid droplet-centered abscess or epithelioid granuloma. Antisera against BCG, B. cereus, and T. pallidum demonstrated bacterial cross-reactive antigens mainly in the lipid droplet surrounded by abscess and/or granuloma (Figure 20) [8, 16, 19]. The antigens were occasionally seen in the cytoplasm of macrophages clustered in the inflammatory lesion. E. coli antigens were infrequently seen in the lesion.

Immunohistochemistry - The Ageless Biotechnology

Splenic bartonellosis (left upper, H&E; right upper, reactivity with Bartonella henselae monoclonal antibody H2A10; left lower, BCG antigens; right lower, T. pallidum antigens). Suppurative granulomas are formed in the spleen. Macrophages in the abscess cavity focally reveal dot-like positive signals not only by the monoclonal antibody but also by antisera against BCG and T. pallidum. Of note is that more signals are seen with the two

Granulomatous mastitis caused by Corynebacterium kroppenstedtii (left upper, H&E; right upper, Gram stain; left lower, BCG antigens; right lower, T. pallidum antigens). This lactation-related infection is microscopically featured by lipid droplet-centered abscess or epithelioid granuloma. Gram stain and immunostaining for BCG, B. cereus, and T. pallidum demonstrate bacterial colonies in the lipid droplet

Figure 19.

Figure 20.

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surrounded by inflammatory reactions.

antisera than with the monoclonal antibody.

Rhinoscleroma is localized and indolent infective nodule in the nasal cavity, endemic in Egypt, South America and eastern Europe. The main causative microbe has been reported to be Klebsiella rhinoscleromatis. Microscopically, foamy macrophages phagocytizing gram-negative rods, so-called Mickulicz cells, are observed in chronic inflammatory infiltrates [44]. A Japanese male aged 70's complained of a single elevated nodule in the nasal vestibule. Biopsy revealed localized infiltration of foamy macrophages in the background of chronic inflammation. Round vacuoles with short rod-like material were occasionally seen in the macrophages or Mickulicz cells [45]. Immunostaining disclosed positive findings with monoclonal antibody 70-2 against Klebsiella spp., and the antiserum against B. cereus gave similar positivity. Fewer cells were also reactive to BCG antiserum. Antisera against T. pallidum and E. coli failed to detect the intracellular microbes (Figure 21). Negativity to E. coli antiserum was an unexpected finding, because of the close relationship between the two enterobacteria.
