Meet the editor

Dr. Charles F. Streckfus is Professor of Biomedical and Diagnostic Sciences at the University of Texas Dental Branch at Houston. Prior to this appointment, he was Assistant Dean of Research and Graduate Programs, University of Mississippi Medical Center. Dr. Streckfus has published over 100 peer-reviewed journal articles. His honors and awards include the prestigious President's Award for Scientific Excellence, presented by the International

Society for Preventive Oncology, 6th International Symposium Predictive Oncology Intervention Strategies, Pasteur Institute, Paris, France, February 12, 2002 for his discovery of salivary Her2/neu as a biomarker for breast cancer. He also received the NIH Award of Merit in 1992.

Contents

**Section 1**

*by Shuji Yamashita*

*by Edwin Roger Parra*

*by Yutaka Tsutsumi*

Extracellular Nucleotides

and Digital Analysis Approaches

**Section 2**

**Preface III**

Technical Aspects of Immunohistochemistry **1**

**Chapter 1 3**

**Chapter 2 29**

Use of Immunohistochemistry in Disease Detection **53**

**Chapter 3 55**

**Chapter 4 69**

**Chapter 5 115**

Immune Cell Profiling in Cancer Using Multiplex Immunofluorescence

Low-Specificity and High-Sensitivity Immunostaining for Demonstrating

Pathogens in Formalin-Fixed, Paraffin-Embedded Sections

In Situ Identification of Ectoenzymes Involved in the Hydrolysis of

*by Mireia Martín-Satué, Aitor Rodríguez-Martínez and Carla Trapero*

*by Sorour Shojaeian, Nasim Maslehat Lay and Amir-Hassan Zarnani*

Detection Systems in Immunohistochemistry

Antigen Retrieval for Light and Electron Microscopy
