**5. Conclusion**

Vaccine application and stringent biosecurity practices helped to suppress the viral load in the flock. As consequences, the morbidity and mortality rate is suppressed, the presentation of clinical signs is milder although the gross pathology features remained consistent. The introduction of H9N2 has initiated the new threat. Egg production drop is today mainly observed as an indication of infection regardless of the virus subtype, although as the latest published active surveillance data (2012–2013) continued blaming H5N1. The masking effect of partial low-level herd immunity may be responsible for the phenomenon.

Virus isolated from chicken with both specific and non-specific lesion between 2003 and 2006 showed high pathogenic avian influenza virus based on molecular marker analysis. Although vaccination has been applied, full viral characterisation continues, evaluation of antibody protective response after vaccination and differentiation between vaccinated and infected birds is needed. Cartography surveillance of avian virus is importantly required to understand cross-immunity of latest strains to use as vaccine seeds. Antigen panel is a must in order to predict future outbreak. Enforcement on regulation for live birds market (LBM) is a must, considering massive human death in China of novel reassortant virus. In addition, wild bird migration from Asia to high densities poultry farms population in Java could increase reassortment rate of circulating virus. Furthermore, the finding of A(H3N8) may trigger novel reassortant virus strain with zoonotic potential. Although, human cluster, Tangerang and Karo, is required for further research since the cases occurred only between people with genetic relation.

#### **Acknowledgements**

The authors would like to express the highest appreciation to Nugroho, DVM, MSc, (Rosa Farm, Blitar, East Java) for supplying the samples.

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**Author details**

Khrisdiana Putri1

Mada, Yogyakarta, Indonesia

Mada, Yogyakarta, Indonesia

provided the original work is properly cited.

\*, Sitarina Widyarini<sup>2</sup>

Universitas Gadjah Mada, Yogyakarta, Indonesia

*The Thrift of Avian Influenza in Indonesia DOI: http://dx.doi.org/10.5772/intechopen.85105*

have no conflict of interest to declare.

**Conflict of interest**

© 2019 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/ by/3.0), which permits unrestricted use, distribution, and reproduction in any medium,

, Sugiyono2

The authors Khrisdiana Putri, Sitarina Widyarini, Sugiyono and Widya Asmara

1 Department of Veterinary Public Health, Faculty of Veterinary Medicine,

2 Department of Pathology, Faculty of Veterinary Medicine, Universitas Gadjah

\*Address all correspondence to: khrisdiana@ugm.ac.id and wied\_as@ugm.ac.id

3 Department of Microbiology, Faculty of Veterinary Medicine, Universitas Gadjah

and Widya Asmara3

\*
