**Acknowledgements**

The authors would like to thank Leopoldo Américo Miguez de Mello, Petrobras Research and Development Center (CENPES) and National Council for Scientific and Technological Development (CNPQ-PIBIC)/Federal University of Rio de Janeiro (UFRJ), for the scholarship financial support and Toyobo of Brazil for the donation of *horseradish peroxidase* enzyme.
