**Author details**

*Legume Crops – Characterization and Breeding for Improved Food Security*

post-transplanting are being monitored (**Figures 8** and **9**).

rooters with synthetic auxins and different types of substrate [54].

**3. Conclusion**

**4. Final remarks**

ecosystem.

editions.

**Acknowledgements**

Project PNHFA-1106094.

At present, survival, annual shoot growth, and different phenological stages

In the adapted specimens, harvesting of the organs of interest will be carried out in the four seasons, coinciding with different crop phenological stages (vegetative, flowering, and fruiting), to measure fresh and dry weight with the aim of determining biomass yield (kg/ha). In the next growing season, the harvested specimens will be evaluated according to regrowth capacity to determine the new available biomass. Likewise, BFP might be reproduced asexually by the vegetative propagation technique, with plant cutting management. Agamic multiplication will be carried out in ligneous and semi-woody nursery cuttings, using different concentrations of

Next-generation domestication: in the age of big data, gene editing, and next generation sequencing (NGS), we have the opportunity to document the transition from wild plant to crop. NGS provides a powerful tool for discovery of domestication genes in crop plants and their wild relatives [55]. The accelerated domestication of new plant species as crops may be facilitated by this knowledge. Re-sequencing of domesticated genotypes can identify regions of low diversity associated with domestication. Novel allelic variation in close or distant relatives can be characterized by NGS; the results give support to the selection and adaptation as new crops and ensure that biomass with therapeutic efficacy will be obtained. The characterization and evaluation of these model species have a phase of construction and validation of genomic cDNA expression libraries with the objective to accelerate the domestication of forest trees in a changing world. This strategy has allowed us to propose plans and technological processes to improve the quality of life in rural territories and to support sustainable growth of wild plant populations and their

This work was supported by the National Institute of Agricultural Technology (INTA), the National Research Council (CONICET) and Faculty of Agronomy and Agri-Food Sciences, University of Moron. This evaluation was included in the

The author also thanks María Monsalvo for her adviser during the figure

**16**

Renée Hersilia Fortunato1 \*, Virginia Fuentes Baluzzi<sup>2</sup> , Fernando De Diego3 , Rodrigo T. Biagioni4 and Alejandro Daniel Esquivel5

1 Faculty of Agronomy and Agri-Food Sciences, University of Moron, Institute of Biological Resources (IRB), Natural Resources Research Center (CIRN), National Institute of Agricultural Technology (INTA), National Research Council (CONICET), Hurlingham, Buenos Aires, Argentina

2 National Research Council (CONICET) and National Institute of Agricultural Technology (INTA), Moreno, Buenos Aires, Argentina

3 Research Council (CONICET) and National Institute of Agricultural Technology (INTA), Institute of Biological Resources (IRB), Hurlingham, Buenos Aires, Argentina

4 Faculty of Agronomy and Agri-Food Sciences, University of Morón, Moron, Argentina

5 Institute of Biological Resources (IRB), Natural Resources Research Center (CIRN), National Institute of Agricultural Technology (INTA), Hurlingham, Buenos Aires, Argentina

\*Address all correspondence to: fortunato.renee@inta.gob.ar

© 2019 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/ by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
