**7. Mango**

The result of [46] study reported an optimized protocol for transient and stable transformation of mango "Carabao" and "Kensington Pride" by biolistic method. They successfully optimized different bombardment parameters (**Table 1**), whereas more than thousand foci were observed per each nucellar proembryonic masses bombarded with a μg plasmid DNA. Afterwards [47], genetically transformed somatic embryos of the three mango varieties Haden, Madame Francis, and Kent with pCAMBIA 3201 construct harboring *gus* and *bar* genes by particle bombardment. After 3 months, only 4% embryos of Kent variety survived, while the other varieties did not survive. They confirmed integration of *gus* and *bar* genes by means of *gus* assay and PCR.
