**Author details**

*The Universe of Escherichia coli*

aa amino acid(s)

β-gal β-galactosidase

Eng endoglucanse Exg exoglucanase *E. coli Escherichia coli*

kDa kilo dalton(s)

Met methionine

Pre- premature

Som somatostatin

Ssp *Synechocystis* species TP target protein

VMA vacuolar ATPase subunit

I Intein

CBD chitin-binding domain *C. fimi Cellulomonas fimi* CNBr cyanogen bromide CV critical value DnaB DnaB helicase

EGF epidermal growth factor

fMet N-formyl-methionine GST glutathione S-transferase

MBP maltose-binding protein

OmpA outer membrane protein A PhoA alkaline phosphatase

PTM post-translational modification

SecYEG trimeric complex of SecY, SecE, and SecG

PspA phage shock protein A

Sce *Saccharomyces cerevisiae* SDA self-driven approach

IPTG isopropyl β-D-1-thiogalactopyranoside

bFGF basic fibroblast growth factor

The authors declare that they have no conflict of interest.

**Conflict of interest**

**Abbreviations**

**40**

Wan-Keung Raymond Wong\*, Ka-Lun Ng, Thiyagarajan Sivakumar, Xiu-Hua Hu, Hao Wang and Lai-Cheuk Nelson Lai Division of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China

\*Address all correspondence to: bcwkrw@ust.hk

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