**3.4 Preparation of the filtrates**

The samples were treated as described by Donkor [25], in which 5 mL of each was taken and mixed with 10 mL of 0.75% trichloroacetic acid (TCA), passing the mixture through filter paper (Whatman No. 1 of 150 mm), obtaining the filtered beverages (FB), which were frozen (−20°C) until analysis. The filtrates were carried out at 0, 7, 14, 21, and 28 days to determine the proteolytic activity, the total peptide concentration, and the antioxidant activity.
