**5. Conclusion**

To be clinically successful, an anticancer drug must have effect over cancer cells in the tumor microenvironment context; however, **in vitro** models do not include all of its components. Therefore, the improvement of cancer models is considered a priority for the current drug development [55].

In the current chapter, we propose a modified air pouch model as an alternative or complement to **in vitro** studies. As previously described, the air pouch model has been extensively used to evaluate inflammation process, anti-inflammatory compounds, immune response, biomaterial compatibility, and of course cancer development and treatment.

The air pouch model allows the administration of higher volumes of chemotherapy alone or combined with other treatment modalities, including targeted therapy, immunotherapy, and biological compounds, to determine single or cumulative antitumor effects, simulating the true clinical condition and treatment.
