**3. Conclusion**

TLM is a powerful and versatile tool in modern biological research, with the immense potential for future clinical applications. One of the probably underexplored features of TLM is its promise to further characterize heterogeneity of cells within tissues [144], in particular, stem/progenitor cells and differentiating cells [299] as well as cancer cells [300]. Some of the above-mentioned methods are associated with unavoidable costs (expensive equipment, such as lenses, filters and sensors, and their damage due to high humidity within the incubator), non-natural impacts on living cells by the high excitation energy of lasers and bleaching/degradation of the fluorochromes over time, which influences quantification of long-running processes. However, the growing number of reports about new improvements and advances in TLM techniques and TLM-related applications that provide valuable information, which is not imageable by other techniques, makes it possible to conclude that the era of microcinematography in biomedical research has just begun.
