Contents



Victoria Moreno-Manzano and Elisa Oltra García



Preface

search and clinical applications of cultured cells.

dimensional environments are discussed.

students.

Cell culture is one of the major tools used in studies of cellular and molecular biology. It delivers an excellent model for studying the normal physiology and biochemistry of cells, and the effects of environment, stresses, drugs, and toxic compounds on the cells' behavior. This book covers some advanced aspects in cell culture methodologies, and medical, re‐

The book comprises four sections that include a total of 12 chapters. In the first section, "Cell Culturing Techniques," different types of cell culture models are discussed, including threedimensional cell culture techniques, their role in drug discovery, together with their advan‐

The second section, "Cell Imaging and Viability," includes autophagy as a cell rescue and cell death method by providing protocols for researchers to investigate the role of autopha‐ gy using an *in vitro* cell line as a model. *In vitro* toxicity tests of nanomaterials on cultured cells and evaluation protocols are accurate, cost-effective, and time-saving methods. Cell imaging is a powerful and versatile tool for monitoring cells using time-lapse microscopy, a real-time imaging of living cells, which points to the fact that modern biology and medicine

The third section, "Stem Cell Culture and Applications," includes stem cell culture for cellbased therapeutics, reviewing the current status of stem cell production and its derivatives, which are paving the way to different treatments in the clinic. Descriptions of two- and three-dimensional cultures and comparison of the behavior of stem cells in two- and three-

Some specific applications and methodologies are shown in the fourth section, which in‐ cludes a description of various approaches that have been used for identifying hybrid cell lines and the results that might be expected when using various technologies for this pur‐ pose. The air-pouch cancer model is a confined tumor microenvironment. It is a modified method for the production of skin substitutes that makes it possible to obtain substitutes that are distinguished by better reproducibility, thus serving as a new tool of choice for pharmacological analyses. The techniques for fabrication of monolayer models and methods

This book provides a comprehensive overview of some of the advanced cell culture method‐ ologies and applications. It serves a valuable source for scientists, researchers, clinicians and

tages and limitations in comparison with traditional two-dimensional culturing.

are entering a thrilling and promising age of molecular cinematography.

used for their studies with a focus on glycolipids are discussed.

