**Author details**

**Tumor growth rate:** Tumor size is defined by the Response Evaluation Criteria in Solid Tumors (RECIST) as the sum of the longest diameters of the tumor mass; to report the tumor growth rate, tumor size can be measured during relevant therapy time points, for example, before treatment, after the first treatment cycle, after the last cycle of treatment, and after discontinuation of the treatment [76]. Also, the Ki67 levels, a protein in all phases of the cell cycle (G1, S, G2, and mitosis), except for the resting phase (G0), often correlate with tumor growth and progression [77]. Tumor growth can be measured with a caliper (volume), or weighted after removal, or determination of Ki67 levels in the tumor tissue by flow cytometry, fluorescence

To be clinically successful, an anticancer drug must have effect over cancer cells in the tumor microenvironment context; however, **in vitro** models do not include all of its components. Therefore, the improvement of cancer models is considered a priority for the current drug

In the current chapter, we propose a modified air pouch model as an alternative or complement to **in vitro** studies. As previously described, the air pouch model has been extensively used to evaluate inflammation process, anti-inflammatory compounds, immune response,

The air pouch model allows the administration of higher volumes of chemotherapy alone or combined with other treatment modalities, including targeted therapy, immunotherapy, and biological compounds, to determine single or cumulative antitumor effects, simulating the

We are grateful for the support to the Laboratory of Immunology and Virology of the Faculty

poly(DTBzl carbonate) poly(desaminotyrosyl-tyrosine benzyl carbonate)

poly(DTE carbonate) poly(desaminotyrosyl-tyrosine ethyl carbonate)

biomaterial compatibility, and of course cancer development and treatment.

microscopy, Western blot, and immunohistochemistry.

**5. Conclusion**

186 Cell Culture

development [55].

true clinical condition and treatment.

**Notes/thanks/other declarations**

The authors declare that there is no conflict of interest.

of Biological Sciences of the Autonomous University of Nuevo León.

**Conflict of interest**

**List of abbreviations**

Moisés Armides Franco-Molina\*, Silvia Elena Santana-Krímskaya and Cristina Rodríguez-Padilla

\*Address all correspondence to: moyfranco@gmail.com

Laboratory of Immunology and Virology of the Faculty of Biological Sciences of the Autonomous University of Nuevo León, San Nicolás de los Garza, México
