**Meet the editor**

Dr. Radwa Mehanna, PhD, is an assistant professor of medical physiology and a stem cell and cell culture laboratories coordinator in the Center of Excellence for Research in Regenerative Medicine and Applications, Faculty of Medicine, Alexandria University. Dr. Radwa has about 12 years of experience in the field of regenerative medicine. She is a member of the Tissue Engineer-

ing and Regenerative Medicine International Society, Tissue Engineering and Regenerative Medicine Egyptian Society, and Egyptian Association of Advancement of Medical Basic Sciences. Dr. Radwa has publications in international peer-reviewed journals in the fields of stem cells and cell culture and runs different projects in the same field.

Contents

**Preface VII**

**Section 1 Cell Culturing Techniques 1**

**Section 2 Cell Imaging and Viability 43**

Chapter 3 **Time-Lapse Microscopy 45**

Kofman

Teow

Chapter 1 **2D vs. 3D Cell Culture Models for In Vitro Topical (Dermatological) Medication Testing 3**

**Culturing in Drug Discovery 21**

Chapter 5 **In Vitro Toxicity Testing of Nanomaterials 97** Ansie Martin and Angshuman Sarkar

**Neuroimmune Applications 111**

Chapter 6 **Culturing Adult Stem Cells for Cell-Based Therapeutics:**

Victoria Moreno-Manzano and Elisa Oltra García

**Section 3 Stem Cell Culture and Applications 109**

Arezou Teimouri, Pollen Yeung and Remigius Agu

Jitcy Saji Joseph, Sibusiso Tebogo Malindisa and Monde Ntwasa

John L. Collins, Bart van Knippenberg, Kai Ding and Alexander V.

Siew-Wai Pang, Noel Jacques Awi, Hooi-Yeen Yap and Sin-Yeang

Chapter 4 **Cell-Based Assays for Evaluation of Autophagy in Cancers 75**

Chapter 2 **Two-Dimensional (2D) and Three-Dimensional (3D) Cell**
