**6. Acknowledgment**

R.P. is recipient of funding from the National Council of Science and Technology, CONACYT (grant CB-2010-CO1-152695) and from the Mexican Institute for Social Security, IMSS (grant FIS/IMSS/852). EDA and EV are scholarship holders from CONACYT.

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**14** 

*Tunisia* 

**Distribution of SDF1-3'A, GNB3 C825T and** 

**from Peripheral Blood of Patients with** 

*1Cellular Immunology and Cytometry and Cellular Therapy Laboratory,* 

**Hematological Malignancies** 

*2Immunology Unit research, Faculty of Pharmacy, Monastir* 

Ben Nasr Moufida2 and Jenhani Faouzi1,2

*National Blood Transfusion Center,* 

**MMP-9 C-1562T Polymorphisms in HSC CD34+** 

Mobilized peripheral blood stem cells (MPBSC) have nearly replaced bone marrow (BM). So, they become the primary source of hematopoietic grafts especially for patients with hematological malignancies undergoing aggressive myelosuppressive or myeloablative chemotherapy. It allows faster engraftment and equivalent disease-free survival compared with bone marrow cells [Siena S et al, 2000; To LB et al, 1997; Roberto M. Lemoli and

Some reports suggested that hematopoietic stem cell mobilization involves a complex interplay between adhesion molecules, cytokines, proteolytic enzymes such as MMP-9 and MMP-2, stromal cells and chemokines among them (e.g,; SDF-1/CXCR4) play a central role [Roberto M. Lemoli and Alessandra D'Addio, 2008; Tsevee Lapidot and Isabelle Petit, 2002]. It has been reported that increased secretion of SDF-1 downmodulates CXCR4 on CD34+ cells, thus preventing the homing of hematopoietic progenitors to the bone marrow [Signoret N et al, 1997]. Moreover, Dlubek D et al, have observed a negative correlation between mobilization capacity and a reduced expression of CXCR4 on mobilized HPC

These data suggested a central role for CXCR4 and SDF-1 on mobilization of hematopoietic

The reason for poor mobilization of hematopoietic stem cells that occur in many donors or patients is fully recognized and patients' characteristics (age, BMI, mobilization regimen,

Benboubker and his colleagues identified an association of a polymorphism in the SDF-1 gene, designated as SDF1-3'A, with the rate of mobilization of HPCs CD34+ into peripheral blood [Benboubker L et al, 2001]. Hence, we hypothesized that individual genetic factors might explain, at least in part, this variability and that polymorphism analysis can be used

stem cell as well as their homing to the bone marrow [Dlubek D et al, 2006].

diagnosis and clinical status or ulterior therapy) did not explain the whole thing.

**1. Introduction** 

Alessandra D'Addio, 2008].

CD34+ in the leukapheresis product [Dlubek D et al, 2006].

to anticipate CD34+ cells mobilization.

