2.2 Preparation of cell suspension and Anti-D IgG samples and sensitization and immunoglobulin binding to the cell surface

A Rho red cell suspension (Red Cell phenotypes R1R1/R1R2 and Rh-rr as control), were diluted to 0.2% in phosphate-buffered saline (PBS) pH 72 and plasma samples, Fraction II and finished product were diluted 1/5 to 1/100 with 1% PBS—pH 7.2.
