2.1 Description of the flow cytometry test

A laser hits the red fluorescein-labeled cells that cross the cytometer in a continuous flow. The light emission produced by the interaction is detected by the forward scatter (FS) and converted into a voltage pulse (FS). A limit of 25,000 events is determined at a flow rate of 600 cells per second with a power of 1300 V. The concentration of Anti-D will be proportional to the size and intensity of fluorescence emitted by the antigen-antibody fluorescein complex of each red blood cell. The mean fluorescence intensity is plotted according to the logarithm of the Anti-D concentration.
